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Rabbit Multiclonal PRAS40 phospho T246 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human AKT1S1 phospho T246.

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Images

Western blot - Anti-PRAS40 (phospho T246) antibody [RP23040013] (AB308042), expandable thumbnail
  • Western blot - Anti-PRAS40 (phospho T246) antibody [RP23040013] (AB308042), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PRAS40 (phospho T246) antibody [RP23040013] (AB308042), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS

Form
Liquid
Clonality
Multiclonal

Immunogen

  • Synthetic Peptide within Human AKT1S1 phospho T246. Database link Q96B36

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Expected
Tested
Mouse
Tested
Expected

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/400
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Negative regulator of the mechanistic target of rapamycin complex 1 (mTORC1), an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth (PubMed:17277771, PubMed:17386266, PubMed:17510057, PubMed:29236692). In absence of insulin and nutrients, AKT1S1 associates with the mTORC1 complex and directly inhibits mTORC1 activity by blocking the MTOR substrate-recruitment site (PubMed:29236692). In response to insulin and nutrients, AKT1S1 dissociates from mTORC1 (PubMed:17386266, PubMed:18372248). Its activity is dependent on its phosphorylation state and binding to 14-3-3 (PubMed:16174443, PubMed:18372248). May also play a role in nerve growth factor-mediated neuroprotection (By similarity).

Alternative names

PRAS40, AKT1S1, Proline-rich AKT1 substrate 1, 40 kDa proline-rich AKT substrate

Recommended products

Rabbit Multiclonal PRAS40 phospho T246 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human AKT1S1 phospho T246.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Multiclonal
Immunogen
  • Synthetic Peptide within Human AKT1S1 phospho T246. Database link Q96B36
Clone number
RP23040013
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.

Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PRAS40 also known as Proline-Rich AKT1 Substrate 40 kDa is a protein that serves a mechanical function as a binding partner within the mTOR complex. This protein with a molecular mass of approximately 40 kDa works within cellular environments to regulate various kinases. PRAS40 expresses ubiquitously but shows significant activity within tissues that have high metabolic roles such as muscle and fat. Its interaction with the mammalian target of rapamycin (mTOR) complex makes it an important component in cellular processes.

Biological function summary

PRAS40 acts as an inhibitory regulator in cellular growth and metabolism. PRAS40 is a part of the mTORC1 complex where it connects with other proteins to modulate the actions of mTOR a central regulator of cellular metabolism. By interacting with the mTORC1 complex PRAS40 can control protein synthesis and cell cycle progression. Furthermore PRAS40 responds to various cellular signals actively contributing to balancing growth factors and nutritional signals in cells.

Pathways

PRAS40 plays a significant role in the mTOR signaling pathway a critical pathway for cell growth and metabolism. PRAS40 regulation within this pathway involves the Akt protein which phosphorylates and modulates PRAS40 activity. Additionally PRAS40 has a connection to insulin signaling pathways where it impacts glucose uptake and energy homeostasis. Through interactions in these pathways PRAS40 balances cellular responses to growth factors and metabolic conditions.

Associated diseases and disorders

PRAS40 shows significant implications in cancer and diabetes. Abnormal regulation of PRAS40 has links to cancer progression due to its role in controlling cell growth via mTOR pathways. Dysregulation in PRAS40 function can enhance cell proliferation and contribute to oncogenesis. Likewise PRAS40 is relevant in diabetes especially through its role in insulin signaling. Imbalances here can disrupt glucose metabolism and exacerbate diabetic conditions. Interactions with proteins such as Akt highlight its potential role in managing these diseases.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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3 product images

  • Western blot - Anti-PRAS40 (phospho T246) antibody [RP23040013] (ab308042), expandable thumbnail

    Western blot - Anti-PRAS40 (phospho T246) antibody [RP23040013] (ab308042)

    Western blot analysis of PRAS40 (phospho T246) in whole cell extracts of NIH-3T3 cells treated with 100 ng/mL PDGF for 15 min using ab308042 at a dilution of 2.5 µg/mL. To confirm specificity, competition was performed by preincubation with the phosphopeptide to inhibit antibody binding (lane 2). Results show a band at ~40 kDa.

    All lanes: Western blot - Anti-PRAS40 (phospho T246) antibody [RP23040013] (ab308042) at 100 ng/mL

    Lane 1: PDGF treated NIH-3T3 whole cell extract at 2.5 µg/mL

    Lane 2: Phosphopeptide and PDGF treated NIH-3T3 whole cell extract at 2.5 µg/mL

    Predicted band size: 27 kDa

    Observed band size: 40 kDa

  • Western blot - Anti-PRAS40 (phospho T246) antibody [RP23040013] (ab308042), expandable thumbnail

    Western blot - Anti-PRAS40 (phospho T246) antibody [RP23040013] (ab308042)

    Western blot analysis of PRAS40 (phospho T246) was performed by loading 30 µg of untreated NIH/3T3 (lane 1) and NIH/3T3 treated with PDGF (100 ng/mL for 15 minutes) lysates (lane 2) using a 4-12% Bis-Tris gel, electrophoresis system and pre-stained protein standard. Proteins were transferred to a nitrocellulose membrane and blocked with 5% skim milk for 1 hour at room temperature. PRAS40 (phospho T246) was detected at ~40 kDa using ab308042 at a 1:1000 dilution in 2.5% skim milk at 4°C overnight on a rocking platform. To confirm specificity, competition was performed with the phosphopeptide (10 µg/mL) (lane 3). Detection was performed using an HRP-conjugated Goat anti-Rabbit secondary antibody at a 1:5000 dilution and chemiluminescent detection was performed (ECL).

    All lanes: Western blot - Anti-PRAS40 (phospho T246) antibody [RP23040013] (ab308042) at 1/1000 dilution

    Lane 1: untreated NIH/3T3 lysate at 30 µg

    Lane 2: PDGF treated NIH/3T3 lysate at 30 µg

    Lane 3: Phosphopeptide and PDGF treated NIH/3T3 lysate at 30 µg

    Secondary

    All lanes: HRP-conjugated Goat anti-Rabbit secondary antibody at 1/5000 dilution

    Developed using the ECL technique.

    Predicted band size: 27 kDa

    Observed band size: 40 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PRAS40 (phospho T246) antibody [RP23040013] (ab308042), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PRAS40 (phospho T246) antibody [RP23040013] (ab308042)

    For immunofluorescence analysis, serum starved HeLa cells treated with 20 ng/mL PDGF for 15 minutes were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature for detection of PRAS40 (phospho T246) using ab308042 at 1/400 in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor® 488 Goat anti-Rabbit IgG Secondary Antibody at a dilution of 1/400 for 30 minutes at room temperature (Panel a: green).

    Nuclei (Panel b: blue) were stained with DAPI

    F-actin (Panel c: red) was stained with Alexa Fluor® 594 Phalloidin

    Panel d is a merged image showing cytoplasmic localization

    Panel e shows competition with the PRAS40 (phospho T246) peptide

    The images were captured at 20X magnification

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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