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AB39069

Anti-PRC1 antibody [6G2]

4

(1 Review)

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(1 Publication)

Mouse Monoclonal PRC1 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Protein regulator of cytokinesis 1 aa 1-150.

View Alternative Names

Protein regulator of cytokinesis 1, PRC1

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

6G2

Isotype

IgG2b

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human Protein regulator of cytokinesis 1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

O43663

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Alternative Products

Primary Antibodies

AB51248

Anti-PRC1 antibody [EP1513Y]

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Flow Cytometry (Intracellular) - Anti-PRC1 antibody [EP1513Y] (AB51248)

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Primary Antibodies

AB62366

Anti-PRC1 (phospho T481) antibody [EP1514Y]

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Western blot - Anti-PRC1 (phospho T481) antibody [EP1514Y] (AB62366)

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Primary Antibodies

AB238427

Anti-PRC1 antibody [EP1513Y] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-PRC1 antibody [EP1513Y] - BSA and Azide free (AB238427)

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Primary Antibodies

AB119338

Anti-PRC1 antibody [16F2]

Immunocytochemistry/ Immunofluorescence - Anti-PRC1 antibody [16F2] (AB119338)

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Complementary Products

Proteins & Peptides

AB106903

Recombinant Human CIRP protein (His tag N-Terminus)

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Assay Kits

AB65622

Phosphate Assay Kit (Colorimetric)

Functional Studies - Phosphate Assay Kit (Colorimetric) (AB65622)

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Reagents, Controls & Accessories

AB131366

VeriBlot for IP Detection Reagent (HRP)

VeriBlot

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

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Primary Antibodies

AB62366

Anti-PRC1 (phospho T481) antibody [EP1514Y]

RabMAb|Recombinant

Western blot - Anti-PRC1 (phospho T481) antibody [EP1514Y] (AB62366)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
ab39069 is an affinity purified IgG.
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PRC1 also known as Protein Regulator of Cytokinesis 1 affiliates with cellular division mechanics. This protein weighs approximately 72 kDa. PRC1 localizes in the nucleus during the interphase and redistributes to the spindle midzone during mitosis. It plays a role in the stabilization of microtubules facilitating proper cytokinesis. Expression of PRC1 occurs in several human tissues notably in dividing cells.
Biological function summary

PRC1 functions in accurately regulating actin and microtubule interaction during cell division. As a part of the centralspindlin complex it ensures correct spindle midzone formation and serves as a marker for the cleavage furrow. Cellular events coordinated by PRC1 involve chromosomal segregation and rapidly proliferating cells express it to maintain integrity during mitotic processes.

Pathways

PRC1 contributes significantly to the mitotic spindle checkpoint. It participates in the Aurora B kinase pathway interacting with proteins like KIF4A and PLK1. This protein ensures the transition from metaphase to anaphase is precise preventing aneuploidy. As a pathway node PRC1 manages signal transduction facilitating cell cycle regulation and fidelity.

Dysregulation of PRC1 expression associates with cancer notably prostate and breast cancer. Tumor cells often display altered PRC1 activity linked to abnormal cell proliferation. The protein interacts with the oncogenic proteins such as p53 indicating a role in tumorigenesis and progression. Exploring therapeutic strategies targeting PRC1 could provide insights into cancer treatment.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Key regulator of cytokinesis that cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody. Required to recruit PLK1 to the spindle. Stimulates PLK1 phosphorylation of RACGAP1 to allow recruitment of ECT2 to the central spindle. Acts as an oncogene for promoting bladder cancer cells proliferation, apoptosis inhibition and carcinogenic progression (PubMed : 17409436).
See full target information Protein regulator of cytokinesis 1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:7799 PubMed40921755

2025

The DNA replication machinery transmits dual signals to prevent unscheduled licensing and execution of centrosome duplication.

Applications

Unspecified application

Species

Unspecified reactive species

Kyohei Matsuhashi,Kei K Ito,Kaho Nagai,Akira Sanada,Koki Watanabe,Kasuga Takumi,Atsushi Toyoda,Masamitsu Fukuyama,Shohei Yamamoto,Takumi Chinen,Grant S Stewart,Shoji Hata,Daiju Kitagawa
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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