AB111851

Anti-PRD antibody

Name: Anti-PRD antibody (ab111851) | Abcam
Brand: Abcam Limited
SKU: AB111851

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(2 Publications)

Rabbit Polyclonal PRD antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PEPD aa 1-300.

View Alternative Names

PRD, PEPD, Xaa-Pro dipeptidase, X-Pro dipeptidase, Imidodipeptidase, Peptidase D, Proline dipeptidase, Prolidase

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRD antibody (AB111851)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRD antibody (AB111851)

ab111851, at a 1/100 dilution, staining PRD in Formalin-fixed, Paraffin-embedded Human fetal liver tissue by Immunohistochemistry.

Unknown

Western blot - Anti-PRD antibody (AB111851)

All lanes:

Western blot - Anti-PRD antibody (ab111851) at 1/500 dilution

Lane 1:

HeLa cell lysate

Lane 2:

HepG2 cell lysate

Predicted band size: 55 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PEPD aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

P12955

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Lyophilized

Reconstitution

Reconstitute in 200 µL of water

Purification technique

Affinity purification Immunogen

Purification notes

ab111851 is purified by a peptide affinity column.

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PRD also known as the podocyte retinoid-dependent domain is a protein of interest in several research areas. This protein has a known mass of approximately 62 kDa. PRD expresses in kidney podocytes specialized cells responsible for filtration in the renal glomerulus. The protein plays an important role mechanically in maintaining podocyte structure and function contributing to the integrity of the glomerular filtration barrier.

Biological function summary

PRD interacts with cellular mechanisms critical for podocyte stability. PRD associates with lipid rafts specialized membrane structures suggesting a role in signal transduction. It functions as part of a larger protein complex that includes nephrin and podocin essential components of the slit diaphragm in podocytes. PRD is involved in cytoskeletal dynamics regulating actin filament organization important for maintaining cell shape and adhesion.

Pathways

Several cellular activities incorporate PRD into their processes. It is closely tied to the PI3K/AKT signaling pathway a critical pathway regulating cell survival and growth. In the slit diaphragm context PRD works alongside nephrin and p130Cas forming a signaling cascade that ensures effective communication between podocytes. These pathways help maintain the health of the glomerular filtration system by responding to mechanical and biochemical stimuli.

The disruption of PRD function links to renal diseases such as nephrotic syndrome and focal segmental glomerulosclerosis (FSGS). Mutations or alterations in PRD expression or interaction can impair the slit diaphragm leading to proteinuria and progressive kidney damage. The protein interacts with nephrin and podocin whose mutations are also implicated in these disorders highlighting its importance in preserving kidney function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Dipeptidase that catalyzes the hydrolysis of dipeptides with a prolyl (Xaa-Pro) or hydroxyprolyl residue in the C-terminal position (PubMed : 17081196, PubMed : 35165443). The preferred dipeptide substrate is Gly-Pro, but other Xaa-Pro dipeptides, such as Ala-Pro, Met-Pro, Phe-Pro, Val-Pro and Leu-Pro, can be cleaved (PubMed : 17081196). Plays an important role in collagen metabolism because the high level of iminoacids in collagen (PubMed : 2925654).

See full target information PEPD

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Medicine 100:e27059 PubMed34477138

2021

Prolidase expression in knee osteoarthritis and healthy controls: Observational study.

Applications

Unspecified application

Species

Unspecified reactive species

Karina Silva-Luna,Lucio Ventura-Ríos,Ambar López-Macay

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 43:313-20 PubMed7868861

1995

Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

J L Whiteland,S M Nicholls,C Shimeld,D L Easty,N A Williams,T J Hill

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-PRD antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRD antibody (AB111851)

Western blot - Anti-PRD antibody (AB111851)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Reconstitution

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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