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Rabbit Recombinant Monoclonal PRD antibody. Carrier free. Suitable for WB and reacts with Human samples.

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Images

Western blot - Anti-PRD antibody [EPR16959] - BSA and Azide free (AB251246), expandable thumbnail
  • Western blot - Anti-PRD antibody [EPR16959] - BSA and Azide free (AB251246), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Target data

Function

Dipeptidase that catalyzes the hydrolysis of dipeptides with a prolyl (Xaa-Pro) or hydroxyprolyl residue in the C-terminal position (PubMed:17081196, PubMed:35165443). The preferred dipeptide substrate is Gly-Pro, but other Xaa-Pro dipeptides, such as Ala-Pro, Met-Pro, Phe-Pro, Val-Pro and Leu-Pro, can be cleaved (PubMed:17081196). Plays an important role in collagen metabolism because the high level of iminoacids in collagen (PubMed:2925654).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal PRD antibody. Carrier free. Suitable for WB and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR16959

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab251246 is the carrier-free version of Anti-PRD antibody [EPR16959] ab197890.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

PRD also known as the podocyte retinoid-dependent domain is a protein of interest in several research areas. This protein has a known mass of approximately 62 kDa. PRD expresses in kidney podocytes specialized cells responsible for filtration in the renal glomerulus. The protein plays an important role mechanically in maintaining podocyte structure and function contributing to the integrity of the glomerular filtration barrier.

Biological function summary

PRD interacts with cellular mechanisms critical for podocyte stability. PRD associates with lipid rafts specialized membrane structures suggesting a role in signal transduction. It functions as part of a larger protein complex that includes nephrin and podocin essential components of the slit diaphragm in podocytes. PRD is involved in cytoskeletal dynamics regulating actin filament organization important for maintaining cell shape and adhesion.

Pathways

Several cellular activities incorporate PRD into their processes. It is closely tied to the PI3K/AKT signaling pathway a critical pathway regulating cell survival and growth. In the slit diaphragm context PRD works alongside nephrin and p130Cas forming a signaling cascade that ensures effective communication between podocytes. These pathways help maintain the health of the glomerular filtration system by responding to mechanical and biochemical stimuli.

Associated diseases and disorders

The disruption of PRD function links to renal diseases such as nephrotic syndrome and focal segmental glomerulosclerosis (FSGS). Mutations or alterations in PRD expression or interaction can impair the slit diaphragm leading to proteinuria and progressive kidney damage. The protein interacts with nephrin and podocin whose mutations are also implicated in these disorders highlighting its importance in preserving kidney function.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-PRD antibody [EPR16959] - BSA and Azide free (ab251246), expandable thumbnail

    Western blot - Anti-PRD antibody [EPR16959] - BSA and Azide free (ab251246)

    This data was developed using Anti-PRD antibody [EPR16959] ab197890, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-PRD antibody [EPR16959] - BSA and Azide free (ab251246) at 1/10000 dilution

    Lane 1: HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg

    Lane 2: SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysate at 20 µg

    Lane 3: 293 (Human epithelial cells from embryonic kidney) whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 55 kDa

  • Western blot - Anti-PRD antibody [EPR16959] - BSA and Azide free (ab251246), expandable thumbnail

    Western blot - Anti-PRD antibody [EPR16959] - BSA and Azide free (ab251246)

    This data was developed using Anti-PRD antibody [EPR16959] ab197890, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-PRD antibody [EPR16959] (Anti-PRD antibody [EPR16959] ab197890) at 1/1000 dilution

    Lane 1: Human fetal kidney lysate at 10 µg

    Lane 2: Human fetal placenta lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 55 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com