Rat Recombinant Monoclonal PRDM1/Blimp1 antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 3 publications.
Images
![Western blot - Anti-PRDM1/Blimp1 antibody [6D3] (AB243146), expandable thumbnail](https://content.abcam.com/products/images/prdm1-blimp1-antibody-6d3-ab243146--western-blot-img92607.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PRDM1/Blimp1 antibody [6D3] (AB243146)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [6D3] (AB243146), expandable thumbnail](https://content.abcam.com/products/images/prdm1-blimp1-antibody-6d3-ab243146--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img56104.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [6D3] (AB243146)")
Publications
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- Immunology 168:580-5962023Regulatory B cells protect against chronic hypoxia-induced pulmonary hypertension by modulating the Tfh/Tfr immune balance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCheng Li,Pingping Liu,Huiling Yao,Hao Zhu,Shaoze Zhang,Fang Meng,San Li,Guang Li,Yanping Peng,Jing Gu,Liming Zhu,Yongliang Jiang,Aiguo DaiPubMed 36221236
- Nature communications 13:76772022PRDM1/BLIMP1 induces cancer immune evasion by modulating the USP22-SPI1-PD-L1 axis in hepatocellular carcinoma cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQing Li,Liren Zhang,Wenhua You,Jiali Xu,Jingjing Dai,Dongxu Hua,Ruizhi Zhang,Feifan Yao,Suiqing Zhou,Wei Huang,Yongjiu Dai,Yu Zhang,Tasiken Baheti,Xiaofeng Qian,Liyong Pu,Jing Xu,Yongxiang Xia,Chuanyong Zhang,Jinhai Tang,Xuehao WangPubMed 36509766
- The Journal of experimental medicine 200:967-772004Plasma cell ontogeny defined by quantitative changes in blimp-1 expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAxel Kallies,Jhagvaral Hasbold,David M Tarlinton,Wendy Dietrich,Lynn M Corcoran,Philip D Hodgkin,Stephen L NuttPubMed 15492122
Key facts
- Isotype
- IgG2a
- Host species
- Rat
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Not recommended | Not recommended |
| Mouse | Tested | Tested |
| Rat | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.89 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 17.8 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Function
Transcription factor that mediates a transcriptional program in various innate and adaptive immune tissue-resident lymphocyte T cell types such as tissue-resident memory T (Trm), natural killer (trNK) and natural killer T (NKT) cells and negatively regulates gene expression of proteins that promote the egress of tissue-resident T-cell populations from non-lymphoid organs (PubMed:27102484). Plays a role in the development, retention and long-term establishment of adaptive and innate tissue-resident lymphocyte T cell types in non-lymphoid organs, such as the skin and gut, but also in other nonbarrier tissues like liver and kidney, and therefore may provide immediate immunological protection against reactivating infections or viral reinfection (PubMed:27102484). Binds specifically to the PRDI element in the promoter of the beta-interferon gene (By similarity). Drives the maturation of B-lymphocytes into Ig secreting cells (By similarity). Associates with the transcriptional repressor ZNF683 to chromatin at gene promoter regions (PubMed:27102484). Binds to the promoter and acts as a transcriptional repressor of IRF8, thereby promotes transcription of osteoclast differentiation factors such as NFATC1 and EEIG1 (PubMed:32741026).
Alternative names
Blimp1, Prdm1, PR domain zinc finger protein 1, B lymphocyte-induced maturation protein 1, Beta-interferon gene positive regulatory domain I-binding factor, PR domain-containing protein 1, Blimp-1
Recommended products
Rat Recombinant Monoclonal PRDM1/Blimp1 antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 3 publications.
Key facts
- Isotype
- IgG2a
- Host species
- Rat
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- 6D3
- Purification technique
- Ion exchange chromatography
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PRDM1 also referred to as Blimp-1 is a transcription factor involved in the regulation of gene expression. It has a molecular mass of approximately 106 kDa. This protein is expressed in a variety of cell types including B lymphocytes T cells and in certain tissues such as the intestine. It plays an important role in controlling the transcriptional programs necessary for cellular differentiation and development. PRDM1 functions mainly by binding to specific DNA sequences to repress the transcription of target genes acting as a master regulator in the immune system.
- Biological function summary
PRDM1/Blimp-1 influences important processes like plasma cell differentiation particularly in the immune response. It is not part of a larger protein complex but functions by recruiting chromatin-modifying enzymes to silence target gene expression. In B cells it promotes the transition to plasma cell formation by repressing genes that would otherwise inhibit this differentiation. Blimp1 also plays a role in maintaining the differentiation state by regulating genes involved in cell survival and function.
- Pathways
PRDM1/Blimp1 is primarily associated with pathways such as the B cell maturation and immunoglobulin secretion pathways. It acts downstream of signaling cascades initiated by factors like CD40 and IL-21. PRDM1 works closely with other transcription factors like XBP-1 in the pathway to drive effective plasma cell differentiation. By repressing factors such as Pax5 and Bcl6 PRDM1 ensures the proper execution of the differentiation program within these pathways.
- Associated diseases and disorders
Malfunctions in PRDM1/Blimp1 are linked to autoimmune conditions like systemic lupus erythematosus and some types of lymphomas. Altered function or expression can disrupt immune homeostasis leading to the production of autoantibodies. Disorders may arise when PRDM1 fails to repress genes like those regulated by Bcl6 contributing to unregulated cell growth and abnormal immune activity. Additionally its dysfunction can be observed in certain gastrointestinal disorders where it is pivotal for maintaining gut immune homeostasis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-PRDM1/Blimp1 antibody [6D3] (ab243146), expandable thumbnail](https://content.abcam.com/products/images/prdm1-blimp1-antibody-6d3-ab243146--western-blot-img92607.jpg "Western blot - Anti-PRDM1/Blimp1 antibody [6D3] (ab243146)")
Western blot - Anti-PRDM1/Blimp1 antibody [6D3] (ab243146)
This blot was developed using a higher sensitivity ECL substrate.
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-PRDM1/Blimp1 antibody [6D3] (ab243146) at 0.89 µg/mL
Lane 1: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg
Lane 2: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) at 1/10000 dilution
Predicted band size: 32 kDa, 91 kDa
Observed band size: 95 kDa
Exposure time: 3min
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [6D3] (ab243146), expandable thumbnail](https://content.abcam.com/products/images/prdm1-blimp1-antibody-6d3-ab243146--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img56104.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [6D3] (ab243146)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [6D3] (ab243146)
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue labeling PRDM1/Blimp1 with ab243146 at 17.8μg/ml followed by ready to use Goat Anti-Rat IgG H&L (HRP). Nuclear staining on the lymph node and surface epithelial cells in the mouse small intestine (PMID: 21878906). Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is ready to use Goat Anti-Rat IgG H&L (HRP).
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
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