AB243146

Anti-PRDM1/Blimp1 antibody [6D3]

Recombinant
What is this?

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(4 Publications)

Rat Recombinant Monoclonal PRDM1/Blimp1 antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 4 publications.

View Alternative Names

Blimp1, Prdm1, PR domain zinc finger protein 1, B lymphocyte-induced maturation protein 1, Beta-interferon gene positive regulatory domain I-binding factor, PR domain-containing protein 1, Blimp-1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [6D3] (AB243146)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [6D3] (AB243146)

Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue labeling PRDM1/Blimp1 with ab243146 at 17.8μg/ml followed by ready to use Goat Anti-Rat IgG H&L (HRP). Nuclear staining on the lymph node and surface epithelial cells in the mouse small intestine (PMID : 21878906). Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use Goat Anti-Rat IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Lab

Western blot - Anti-PRDM1/Blimp1 antibody [6D3] (AB243146)

This blot was developed using a higher sensitivity ECL substrate.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PRDM1/Blimp1 antibody [6D3] (ab243146) at 0.89 µg/mL

Lane 1:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

Lane 2:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/10000 dilution

Predicted band size: 32 kDa,91 kDa

Observed band size: 95 kDa

false

Exposure time: 3min

Carrier free

Anti-PRDM1/Blimp1 antibody [6D3] - BSA and Azide free

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

6D3

Isotype

IgG2a

Light chain type

kappa

Carrier free

Reacts with

Mouse

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "0.89 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "17.8 µg/mL", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.89 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "17.8 µg/mL", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "0.89 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "17.8 µg/mL", "IHCP-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Ion exchange chromatography

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PRDM1 also referred to as Blimp-1 is a transcription factor involved in the regulation of gene expression. It has a molecular mass of approximately 106 kDa. This protein is expressed in a variety of cell types including B lymphocytes T cells and in certain tissues such as the intestine. It plays an important role in controlling the transcriptional programs necessary for cellular differentiation and development. PRDM1 functions mainly by binding to specific DNA sequences to repress the transcription of target genes acting as a master regulator in the immune system.

Biological function summary

PRDM1/Blimp-1 influences important processes like plasma cell differentiation particularly in the immune response. It is not part of a larger protein complex but functions by recruiting chromatin-modifying enzymes to silence target gene expression. In B cells it promotes the transition to plasma cell formation by repressing genes that would otherwise inhibit this differentiation. Blimp1 also plays a role in maintaining the differentiation state by regulating genes involved in cell survival and function.

Pathways

PRDM1/Blimp1 is primarily associated with pathways such as the B cell maturation and immunoglobulin secretion pathways. It acts downstream of signaling cascades initiated by factors like CD40 and IL-21. PRDM1 works closely with other transcription factors like XBP-1 in the pathway to drive effective plasma cell differentiation. By repressing factors such as Pax5 and Bcl6 PRDM1 ensures the proper execution of the differentiation program within these pathways.

Malfunctions in PRDM1/Blimp1 are linked to autoimmune conditions like systemic lupus erythematosus and some types of lymphomas. Altered function or expression can disrupt immune homeostasis leading to the production of autoantibodies. Disorders may arise when PRDM1 fails to repress genes like those regulated by Bcl6 contributing to unregulated cell growth and abnormal immune activity. Additionally its dysfunction can be observed in certain gastrointestinal disorders where it is pivotal for maintaining gut immune homeostasis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that mediates a transcriptional program in various innate and adaptive immune tissue-resident lymphocyte T cell types such as tissue-resident memory T (Trm), natural killer (trNK) and natural killer T (NKT) cells and negatively regulates gene expression of proteins that promote the egress of tissue-resident T-cell populations from non-lymphoid organs (PubMed : 27102484). Plays a role in the development, retention and long-term establishment of adaptive and innate tissue-resident lymphocyte T cell types in non-lymphoid organs, such as the skin and gut, but also in other nonbarrier tissues like liver and kidney, and therefore may provide immediate immunological protection against reactivating infections or viral reinfection (PubMed : 27102484). Binds specifically to the PRDI element in the promoter of the beta-interferon gene (By similarity). Drives the maturation of B-lymphocytes into Ig secreting cells (By similarity). Associates with the transcriptional repressor ZNF683 to chromatin at gene promoter regions (PubMed : 27102484). Binds to the promoter and acts as a transcriptional repressor of IRF8, thereby promotes transcription of osteoclast differentiation factors such as NFATC1 and EEIG1 (PubMed : 32741026).

See full target information Prdm1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:159 PubMed39901028

2025

Arginine methyltransferase PRMT1 promotes ferroptosis through EGR1/GLS2 axis in sepsis-related acute lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Min Li,Longhui Hu,Qiao Ke,Zhao Li,Chujun Ruan,Hanjing Lu,Xiaoran Liu

Immunology 168:580-596 PubMed36221236

2023

Regulatory B cells protect against chronic hypoxia-induced pulmonary hypertension by modulating the Tfh/Tfr immune balance.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Li,Pingping Liu,Huiling Yao,Hao Zhu,Shaoze Zhang,Fang Meng,San Li,Guang Li,Yanping Peng,Jing Gu,Liming Zhu,Yongliang Jiang,Aiguo Dai

Nature communications 13:7677 PubMed36509766

2022

PRDM1/BLIMP1 induces cancer immune evasion by modulating the USP22-SPI1-PD-L1 axis in hepatocellular carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Li,Liren Zhang,Wenhua You,Jiali Xu,Jingjing Dai,Dongxu Hua,Ruizhi Zhang,Feifan Yao,Suiqing Zhou,Wei Huang,Yongjiu Dai,Yu Zhang,Tasiken Baheti,Xiaofeng Qian,Liyong Pu,Jing Xu,Yongxiang Xia,Chuanyong Zhang,Jinhai Tang,Xuehao Wang

The Journal of experimental medicine 200:967-77 PubMed15492122

2004

Plasma cell ontogeny defined by quantitative changes in blimp-1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Axel Kallies,Jhagvaral Hasbold,David M Tarlinton,Wendy Dietrich,Lynn M Corcoran,Philip D Hodgkin,Stephen L Nutt

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PRDM1/Blimp1 antibody [6D3]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [6D3] (AB243146)

Western blot - Anti-PRDM1/Blimp1 antibody [6D3] (AB243146)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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Primary Antibodies

Primary Antibodies

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