Anti-PRDM1/Blimp1 antibody [6D3] - BSA and Azide free

• WB

Lab

Western blot - Anti-PRDM1/Blimp1 antibody [6D3] - BSA and Azide free (AB252242)

This blot was developed using a higher sensitivity ECL substrate.

Blocking/Dilution buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243146).

All lanes:

Western blot - Anti-PRDM1/Blimp1 antibody [6D3] (<a href='/en-us/products/primary-antibodies/prdm1-blimp1-antibody-6d3-ab243146'>ab243146</a>) at 0.89 µg/mL

Lane 1:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

Lane 2:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/10000 dilution

Predicted band size: 32 kDa,91 kDa

Observed band size: 95 kDa

false

Exposure time: 3min

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [6D3] - BSA and Azide free (AB252242)

Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue labeling PRDM1/Blimp1 with ab243146 at 17.8μg/ml followed by ready to use Goat Anti-Rat IgG H&L (HRP). Nuclear staining on the lymph node and surface epithelial cells in the mouse small intestine (PMID : 21878906). Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use Goat Anti-Rat IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243146).