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AB119401

Anti-PRDM1/Blimp1 antibody

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(3 Publications)

Rabbit Polyclonal PRDM1/Blimp1 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PRDM1 aa 100-150.

View Alternative Names

BLIMP1, PRDM1, PR domain zinc finger protein 1, BLIMP-1, Beta-interferon gene positive regulatory domain I-binding factor, PR domain-containing protein 1, Positive regulatory domain I-binding factor 1, PRDI-BF1, PRDI-binding factor 1

1 Images
Western blot - Anti-PRDM1/Blimp1 antibody (AB119401)
  • WB

Unknown

Western blot - Anti-PRDM1/Blimp1 antibody (AB119401)

All lanes:

Western blot - Anti-PRDM1/Blimp1 antibody (ab119401) at 1/500 dilution

Lane 1:

Jurkat cell extract

Lane 2:

Raji cell extract

Predicted band size: 91 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human PRDM1 aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

O75626

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PRDM1 also referred to as Blimp-1 is a transcription factor involved in the regulation of gene expression. It has a molecular mass of approximately 106 kDa. This protein is expressed in a variety of cell types including B lymphocytes T cells and in certain tissues such as the intestine. It plays an important role in controlling the transcriptional programs necessary for cellular differentiation and development. PRDM1 functions mainly by binding to specific DNA sequences to repress the transcription of target genes acting as a master regulator in the immune system.
Biological function summary

PRDM1/Blimp-1 influences important processes like plasma cell differentiation particularly in the immune response. It is not part of a larger protein complex but functions by recruiting chromatin-modifying enzymes to silence target gene expression. In B cells it promotes the transition to plasma cell formation by repressing genes that would otherwise inhibit this differentiation. Blimp1 also plays a role in maintaining the differentiation state by regulating genes involved in cell survival and function.

Pathways

PRDM1/Blimp1 is primarily associated with pathways such as the B cell maturation and immunoglobulin secretion pathways. It acts downstream of signaling cascades initiated by factors like CD40 and IL-21. PRDM1 works closely with other transcription factors like XBP-1 in the pathway to drive effective plasma cell differentiation. By repressing factors such as Pax5 and Bcl6 PRDM1 ensures the proper execution of the differentiation program within these pathways.

Malfunctions in PRDM1/Blimp1 are linked to autoimmune conditions like systemic lupus erythematosus and some types of lymphomas. Altered function or expression can disrupt immune homeostasis leading to the production of autoantibodies. Disorders may arise when PRDM1 fails to repress genes like those regulated by Bcl6 contributing to unregulated cell growth and abnormal immune activity. Additionally its dysfunction can be observed in certain gastrointestinal disorders where it is pivotal for maintaining gut immune homeostasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that mediates a transcriptional program in various innate and adaptive immune tissue-resident lymphocyte T cell types such as tissue-resident memory T (Trm), natural killer (trNK) and natural killer T (NKT) cells and negatively regulates gene expression of proteins that promote the egress of tissue-resident T-cell populations from non-lymphoid organs. Plays a role in the development, retention and long-term establishment of adaptive and innate tissue-resident lymphocyte T cell types in non-lymphoid organs, such as the skin and gut, but also in other nonbarrier tissues like liver and kidney, and therefore may provide immediate immunological protection against reactivating infections or viral reinfection (By similarity). Binds specifically to the PRDI element in the promoter of the beta-interferon gene (PubMed : 1851123). Drives the maturation of B-lymphocytes into Ig secreting cells (PubMed : 12626569). Associates with the transcriptional repressor ZNF683 to chromatin at gene promoter regions (By similarity). Binds to the promoter and acts as a transcriptional repressor of IRF8, thereby promotes transcription of osteoclast differentiation factors such as NFATC1 and EEIG1 (By similarity).
See full target information PRDM1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 19:112 PubMed33731128

2021

KPNA2 interaction with CBX8 contributes to the development and progression of bladder cancer by mediating the PRDM1/c-FOS pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Fanchang Zeng,Liumei Luo,Daoyuan Li,Juncheng Guo,Min Guo

FASEB journal : official publication of the Federa 33:12780-12799 PubMed31480861

2019

T-cell-derived extracellular vesicles regulate B-cell IgG production pyruvate kinase muscle isozyme 2.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Yang,Guohui Dang,Silin Lü,Huiying Liu,Xiaolong Ma,Lulu Han,Jiacheng Deng,Yutong Miao,Xiaopeng Li,Fangyu Shao,Changtao Jiang,Qingbo Xu,Xian Wang,Juan Feng

International journal of molecular medicine 37:1601-10 PubMed27081781

2016

Suppression of SIK1 by miR-141 in human ovarian cancer cell lines and tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Jin-Long Chen,Fang Chen,Ting-Ting Zhang,Nai-Fu Liu
View all publications
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