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AB13700

Anti-PRDM1/Blimp1 antibody

4

(5 Reviews)

|

(10 Publications )

Goat Polyclonal PRDM1/Blimp1 antibody. Suitable for ICC, ChIP, Flow Cyt and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human PRDM1 aa 800-850.

View Alternative Names

BLIMP1, PRDM1, PR domain zinc finger protein 1, BLIMP-1, Beta-interferon gene positive regulatory domain I-binding factor, PR domain-containing protein 1, Positive regulatory domain I-binding factor 1, PRDI-BF1, PRDI-binding factor 1

1 Images
Immunocytochemistry - Anti-PRDM1/Blimp1 antibody (AB13700)
  • ICC

Unknown

Immunocytochemistry - Anti-PRDM1/Blimp1 antibody (AB13700)

Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC, Flow Cyt, ChIP

applications

Immunogen

Synthetic Peptide within Human PRDM1 aa 800-850. The exact immunogen used to generate this antibody is proprietary information.

O75626

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "10 µg/mL", "ICC-species-notes": "<p></p>", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/goat-igg-polyclonal-isotype-control-ab37373'>ab37373</a> - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Rat": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Cow": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Dog": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Pig": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PRDM1 also referred to as Blimp-1 is a transcription factor involved in the regulation of gene expression. It has a molecular mass of approximately 106 kDa. This protein is expressed in a variety of cell types including B lymphocytes T cells and in certain tissues such as the intestine. It plays an important role in controlling the transcriptional programs necessary for cellular differentiation and development. PRDM1 functions mainly by binding to specific DNA sequences to repress the transcription of target genes acting as a master regulator in the immune system.
Biological function summary

PRDM1/Blimp-1 influences important processes like plasma cell differentiation particularly in the immune response. It is not part of a larger protein complex but functions by recruiting chromatin-modifying enzymes to silence target gene expression. In B cells it promotes the transition to plasma cell formation by repressing genes that would otherwise inhibit this differentiation. Blimp1 also plays a role in maintaining the differentiation state by regulating genes involved in cell survival and function.

Pathways

PRDM1/Blimp1 is primarily associated with pathways such as the B cell maturation and immunoglobulin secretion pathways. It acts downstream of signaling cascades initiated by factors like CD40 and IL-21. PRDM1 works closely with other transcription factors like XBP-1 in the pathway to drive effective plasma cell differentiation. By repressing factors such as Pax5 and Bcl6 PRDM1 ensures the proper execution of the differentiation program within these pathways.

Malfunctions in PRDM1/Blimp1 are linked to autoimmune conditions like systemic lupus erythematosus and some types of lymphomas. Altered function or expression can disrupt immune homeostasis leading to the production of autoantibodies. Disorders may arise when PRDM1 fails to repress genes like those regulated by Bcl6 contributing to unregulated cell growth and abnormal immune activity. Additionally its dysfunction can be observed in certain gastrointestinal disorders where it is pivotal for maintaining gut immune homeostasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that mediates a transcriptional program in various innate and adaptive immune tissue-resident lymphocyte T cell types such as tissue-resident memory T (Trm), natural killer (trNK) and natural killer T (NKT) cells and negatively regulates gene expression of proteins that promote the egress of tissue-resident T-cell populations from non-lymphoid organs. Plays a role in the development, retention and long-term establishment of adaptive and innate tissue-resident lymphocyte T cell types in non-lymphoid organs, such as the skin and gut, but also in other nonbarrier tissues like liver and kidney, and therefore may provide immediate immunological protection against reactivating infections or viral reinfection (By similarity). Binds specifically to the PRDI element in the promoter of the beta-interferon gene (PubMed : 1851123). Drives the maturation of B-lymphocytes into Ig secreting cells (PubMed : 12626569). Associates with the transcriptional repressor ZNF683 to chromatin at gene promoter regions (By similarity). Binds to the promoter and acts as a transcriptional repressor of IRF8, thereby promotes transcription of osteoclast differentiation factors such as NFATC1 and EEIG1 (By similarity).
See full target information PRDM1
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:7677 PubMed36509766

2022

PRDM1/BLIMP1 induces cancer immune evasion by modulating the USP22-SPI1-PD-L1 axis in hepatocellular carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Li,Liren Zhang,Wenhua You,Jiali Xu,Jingjing Dai,Dongxu Hua,Ruizhi Zhang,Feifan Yao,Suiqing Zhou,Wei Huang,Yongjiu Dai,Yu Zhang,Tasiken Baheti,Xiaofeng Qian,Liyong Pu,Jing Xu,Yongxiang Xia,Chuanyong Zhang,Jinhai Tang,Xuehao Wang

Chemotherapy 67:12-23 PubMed34844236

2021

Dysregulated MDR1 by PRDM1/Blimp1 Is Involved in the Doxorubicin Resistance of Non-Germinal Center B-Cell-Like Diffuse Large B-Cell Lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Qing,Zhen Jin,Zizhen Xu,Wenfang Wang,Xiaoyang Li,Yunxiang Zhang,Lining Wang,Hongming Zhu,Rufang Xiang,Shishuang Wu,Ran Li,Ge Jiang,Kai Xue,Junmin Li

Development (Cambridge, England) 146: PubMed31806661

2019

PRDM1 controls the sequential activation of neural, neural crest and sensory progenitor determinants.

Applications

Unspecified application

Species

Unspecified reactive species

Ravindra S Prajapati,Mark Hintze,Andrea Streit

Scientific reports 9:12552 PubMed31467373

2019

TGF-β1 increases viral burden and promotes HIV-1 latency in primary differentiated human bronchial epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

S Chinnapaiyan,R K Dutta,M Nair,H S Chand,I Rahman,H J Unwalla

Developmental biology 455:32-41 PubMed31271752

2019

PRDM14 and BLIMP1 control the development of chicken primordial germ cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yuya Okuzaki,Hidenori Kaneoka,Takayuki Suzuki,Yota Hagihara,Yuki Nakayama,Seitaro Murakami,Yusuke Murase,Atsushi Kuroiwa,Shinji Iijima,Ken-Ichi Nishijima

Scientific reports 5:7913 PubMed25604641

2015

GPR39 marks specific cells within the sebaceous gland and contributes to skin wound healing.

Applications

IF

Species

Unspecified reactive species

Huashan Zhao,Jingqiao Qiao,Shoubing Zhang,Huishan Zhang,Xiaohua Lei,Xinyue Wang,Zhili Deng,Lina Ning,Yujing Cao,Yong Guo,Shuang Liu,Enkui Duan

PloS one 5:e9951 PubMed20376350

2010

Prdm1 (Blimp-1) and the expression of fast and slow myosin heavy chain isoforms during avian myogenesis in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Mary Lou Beermann,Magdalena Ardelt,Mahasweta Girgenrath,Jeffrey Boone Miller

Molecular and cellular biology 29:3832-44 PubMed19433448

2009

RelB NF-kappaB represses estrogen receptor alpha expression via induction of the zinc finger protein Blimp1.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaobo Wang,Karine Belguise,Christine F O'Neill,Nuria Sánchez-Morgan,Mathilde Romagnoli,Sean F Eddy,Nora D Mineva,Ziyang Yu,Chengyin Min,Vickery Trinkaus-Randall,Dany Chalbos,Gail E Sonenshein

Blood 110:2067-74 PubMed17545502

2007

BCL6 programs lymphoma cells for survival and differentiation through distinct biochemical mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Samir Parekh,Jose M Polo,Rita Shaknovich,Przemyslaw Juszczynski,Paola Lev,Stella M Ranuncolo,Yingnan Yin,Ulf Klein,Giorgio Cattoretti,Riccardo Dalla Favera,Margaret A Shipp,Ari Melnick

Proceedings of the National Academy of Sciences of 104:1841-6 PubMed17264218

2007

BLIMP1 regulates cell growth through repression of p53 transcription.

Applications

ChIP

Species

Human

Junli Yan,Jianming Jiang,Ching Aeng Lim,Qiang Wu,Huck-Hui Ng,Keh-Chuang Chin
View all publications
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