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AB230867

Anti-PRDM13 antibody - N-terminal

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(1 Publication)

Rabbit Polyclonal PRDM13 antibody. N-terminal. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PRDM13 aa 1-50 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

PFM10, PRDM13, PR domain zinc finger protein 13, PR domain-containing protein 13

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM13 antibody - N-terminal (AB230867)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM13 antibody - N-terminal (AB230867)

Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for PRDM13 using ab230867 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM13 antibody - N-terminal (AB230867)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM13 antibody - N-terminal (AB230867)

Formalin-fixed, paraffin-embedded human kidney tissue stained for PRDM13 using ab230867 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-PRDM13 antibody - N-terminal (AB230867)
  • WB

Supplier Data

Western blot - Anti-PRDM13 antibody - N-terminal (AB230867)

All lanes:

Western blot - Anti-PRDM13 antibody - N-terminal (ab230867) at 1/1000 dilution

All lanes:

Human ovary tissue lysate at 35 µg

Secondary

All lanes:

Goat anti-rabbit IgG H&L (HRP) at 1/10000 dilution

Predicted band size: 75 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human PRDM13 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9H4Q3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PRDM13 often referred to as PR domain containing 13 plays an important role in the regulation of gene expression through its action as a transcriptional repressor. It acts by binding to specific regions of DNA and modifying chromatin structure to suppress or silence gene activity. PRDM13 with its molecular mass of approximately 45 kDa is predominantly expressed in the developing neural tissue particularly during early embryogenesis. Its expression is also detected in certain adult tissues but to a lesser extent.
Biological function summary

This protein contributes to the establishment of neuronal identity and differentiation. PRDM13 often operates as a part of larger protein complexes that are involved in transcriptional repression. By interacting with other proteins it fine-tunes gene expression to ensure proper development of the central nervous system. Its precise regulation during critical windows of embryonic development suggests it molds the fate of progenitor cells into specific neuronal lineages highlighting its significance in neural patterning.

Pathways

PRDM13 plays an essential role in the Notch signaling pathway which is instrumental in maintaining the balance between proliferation and differentiation in neural progenitor cells. It strategically regulates this path in cooperation with other related proteins like Hes1 and Sox2. PRDM13 indirectly influences the activity of involved genes ensuring that the cellular responses are appropriate for developmental stages. This modulation can ensure the correct morphological and functional development of the nervous system.

Aberrant expression or mutation of PRDM13 associates with neurodevelopmental disorders such as intellectual disability and related cognitive impairments. PRDM13's connection to diseases stems from its interactions with proteins like NOTCH1 as disruptions in these pathways may lead to improper neuronal differentiation and development. Ongoing research aims to decipher its exact role and how its dysfunction contributes to these conditions paving the way for potential therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be involved in transcriptional regulation. Is required for the differentiation of KISS1-expressing neurons in the arcuate (Arc) nucleus of the hypothalamus. Is a critical regulator of GABAergic cell fate in the cerebellum, required for normal postnatal cerebellar development (By similarity).
See full target information PRDM13
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 134: PubMed38885336

2024

The IFITM5 mutation in osteogenesis imperfecta type V is associated with an ERK/SOX9-dependent osteoprogenitor differentiation defect.

Applications

Unspecified application

Species

Unspecified reactive species

Ronit Marom,I-Wen Song,Emily C Busse,Megan E Washington,Ava S Berrier,Vittoria C Rossi,Laura Ortinau,Youngjae Jeong,Ming-Ming Jiang,Brian C Dawson,Mary Adeyeye,Carolina Leynes,Caressa D Lietman,Bridget M Stroup,Dominyka Batkovskyte,Mahim Jain,Yuqing Chen,Racel Cela,Alexis Castellon,Alyssa A Tran,Isabel Lorenzo,D Nicole Meyers,Shixia Huang,Alicia Turner,Vinitha Shenava,Maegen Wallace,Eric Orwoll,Dongsu Park,Catherine G Ambrose,Sandesh Cs Nagamani,Jason D Heaney,Brendan H Lee
View all publications
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