Anti-PRDM16 antibody

• WB

Supplier Data

Western blot - Anti-PRDM16 antibody (AB106410)

1 h incubation at RT in 5% NFDM/TBST.

All lanes:

Western blot - Anti-PRDM16 antibody (ab106410) at 1 µg/mL

All lanes:

MCF7 cell lysates at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution

Observed band size: 130 kDa

false

• ICC

AbReview39070****

Immunocytochemistry - Anti-PRDM16 antibody (AB106410)

ab106410 staining RAP1 in Human Brown adipocytes from supraclavicular fat tissue by ICC (Immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 10% serum, 1% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (2 ug/ml in 10% serum, 1% BSA, 0.3% Triton X100, PBS) for 16 hours at 4°C. A Cy3®-conjugated Donkey F(ab')2 anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

Image is courtesy of an Abreview submitted by Lena Appelsved

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM16 antibody (AB106410)

Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-PRDM16 antibody (ab106410) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM16 antibody (AB106410)

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PRDM16 antibody (ab106410) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM16 antibody (AB106410)

Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-PRDM16 antibody (ab106410) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM16 antibody (AB106410)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PRDM16 antibody (ab106410) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

• WB

Supplier Data

Western blot - Anti-PRDM16 antibody (AB106410)

1 h incubation at RT in 5% NFDM/TBST.

All lanes:

Western blot - Anti-PRDM16 antibody (ab106410) at 2 µg/mL

All lanes:

Rat spleen tissue lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution

Observed band size: 130 kDa

false

• WB

Supplier Data

Western blot - Anti-PRDM16 antibody (AB106410)

1 h incubation at RT in 5% NFDM/TBST.

All lanes:

Western blot - Anti-PRDM16 antibody (ab106410) at 2 µg/mL

All lanes:

Mouse lung tissue lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution

Observed band size: 130 kDa

false