Rabbit Polyclonal PRDM16 antibody. Suitable for ICC, WB, IHC-P and reacts with Human, Rat, Mouse samples. Cited in 79 publications. Immunogen corresponding to Synthetic Peptide within Human PRDM16.
Images
Publications
Filter by
- Foods (Basel, Switzerland) 12:2023Effect of 2'-Fucosyllactose on Beige Adipocyte Formation in 3T3-L1 Adipocytes and C3H10T1/2 Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSiru Chen,Yankun Fu,Tianlin Wang,Zhenglin Chen,Peijun Zhao,Xianqing Huang,Mingwu Qiao,Tiange Li,Lianjun SongPubMed 38002194
- Foods (Basel, Switzerland) 12:2023from Fu Brick Tea Promotes Adipose Thermogenesis by Boosting Colonic in High-Fat-Fed Obese Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Wang,Ting Li,Chengcheng Yang,Yingmei Wu,Yueyue Liu,Xingbin YangPubMed 37893609
- Nature communications 14:59712023A common East-Asian ALDH2 mutation causes metabolic disorders and the therapeutic effect of ALDH2 activators.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi-Cheng Chang,Hsiao-Lin Lee,Wenjin Yang,Meng-Lun Hsieh,Cai-Cin Liu,Tung-Yuan Lee,Jing-Yong Huang,Jiun-Yi Nong,Fu-An Li,Hsiao-Li Chuang,Zhi-Zhong Ding,Wei-Lun Su,Li-Yun Chueh,Yi-Ting Tsai,Che-Hong Chen,Daria Mochly-Rosen,Lee-Ming ChuangPubMed 37749090
- International journal of molecular medicine 52:2023root decreases lipid accumulation through the induction of lipolysis and thermogenesis via AMPK activation in 3T3‑L1 cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJeong Won Choi,Hyeok Jin Choi,Gwang Hyeon Ryu,Jae Won Lee,Jueng Kyu Beak,Eun Jeong Koh,Jin Boo JeongPubMed 37326061
- JCI insight 8:2023PRDM16 deficiency in vascular smooth muscle cells aggravates abdominal aortic aneurysm.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenguo Wang,Xiangjie Zhao,Guizhen Zhao,Yanhong Guo,Haocheng Lu,Wenjuan Mu,Juan Zhong,Minerva Garcia-Barrio,Jifeng Zhang,Y Eugene Chen,Lin ChangPubMed 37079380
- Nutrients 15:2023β-Hydroxy-β-methyl Butyrate Regulates the Lipid Metabolism, Mitochondrial Function, and Fat Browning of Adipocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGeyan Duan,Changbing Zheng,Jiayi Yu,Peiwen Zhang,Mengliao Wan,Jie Zheng,Yehui DuanPubMed 37299513
- Experimental and therapeutic medicine 26:3332023Inhibition of lipid droplet accumulation by by suppressing adipogenesis and inducing lipolysis, thermogenesis and autophagy in 3T3‑L1 cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJeong Won Choi,Hyeok Jin Choi,Gwang Hyeon Ryu,Jae Won Lee,Jin Boo JeongPubMed 37346399
- Experimental and therapeutic medicine 25:2192023Immunostimulatory and anti‑obesity activities of leaves in RAW264.7 and 3T3‑L1 cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyeok Jin Choi,Jeong Won Choi,Seung Woo Im,Jin Boo JeongPubMed 37123212
- The Journal of cell biology 222:2023Antagonism between Prdm16 and Smad4 specifies the trajectory and progression of pancreatic cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEric Hurwitz,Parash Parajuli,Seval Ozkan,Celine Prunier,Thien Ly Nguyen,Deanna Campbell,Creighton Friend,Allyn Austin Bryan,Ting-Xuan Lu,Steven Christopher Smith,Mohammed Shawkat Razzaque,Keli Xu,Azeddine AtfiPubMed 36828547
- International journal of molecular sciences 23:2022Cucurbitacin B-, E-, and I-Induced Browning of White Adipocytes Is Promoted by the Inhibition of Phospholipase D2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSun Young Park,Hye Mi Kang,Jin-Woo Oh,Young-Whan Choi,Geuntae ParkPubMed 36499689
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human PRDM16. The exact immunogen used to generate this antibody is proprietary information. Database link Q9HAZ2
Reactivity data
Select an application| ICC | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Tested |
| Rat | Expected | Tested | Tested |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-4 µg/mL | Notes - |
Species Rat | Dilution info 1-4 µg/mL | Notes - |
Species Mouse | Dilution info 1-4 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes - |
Species Rat | Dilution info 1-2 µg/mL | Notes - |
Species Mouse | Dilution info 1-2 µg/mL | Notes - |
Target data
Function
Binds DNA and functions as a transcriptional regulator (PubMed:12816872). Displays histone methyltransferase activity and monomethylates 'Lys-9' of histone H3 (H3K9me1) in vitro (By similarity). Probably catalyzes the monomethylation of free histone H3 in the cytoplasm which is then transported to the nucleus and incorporated into nucleosomes where SUV39H methyltransferases use it as a substrate to catalyze histone H3 'Lys-9' trimethylation (By similarity). Likely to be one of the primary histone methyltransferases along with MECOM/PRDM3 that direct cytoplasmic H3K9me1 methylation (By similarity). Functions in the differentiation of brown adipose tissue (BAT) which is specialized in dissipating chemical energy in the form of heat in response to cold or excess feeding while white adipose tissue (WAT) is specialized in the storage of excess energy and the control of systemic metabolism (By similarity). Together with CEBPB, regulates the differentiation of myoblastic precursors into brown adipose cells (By similarity). Functions as a repressor of TGF-beta signaling (PubMed:19049980). Isoform 4. Binds DNA and functions as a transcriptional regulator (PubMed:12816872). Functions as a repressor of TGF-beta signaling (PubMed:14656887). May regulate granulocyte differentiation (PubMed:12816872).
Alternative names
KIAA1675, MEL1, PFM13, PRDM16, Histone-lysine N-methyltransferase PRDM16, PR domain zinc finger protein 16, PR domain-containing protein 16, Transcription factor MEL1, MDS1/EVI1-like gene 1
Recommended products
Rabbit Polyclonal PRDM16 antibody. Suitable for ICC, WB, IHC-P and reacts with Human, Rat, Mouse samples. Cited in 79 publications. Immunogen corresponding to Synthetic Peptide within Human PRDM16.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human PRDM16. The exact immunogen used to generate this antibody is proprietary information. Database link Q9HAZ2
- Purification technique
- Affinity purification Immunogen
- Specificity
At least four isoforms of PRDM16 are known to exist; ab106410 will recognize all four.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PRDM16 also known as the PR-domain containing 16 is a transcription cofactor involved in the regulation of genes. It has a mass of approximately 140 kDa. This protein is found in multiple tissues with high expression levels observed in brown adipose tissue and skeletal muscle. PRDM16 regulates gene expression by interacting with other transcription factors and modifying chromatin structure.
- Biological function summary
PRDM16 is essential in the determination of brown adipose tissue and skeletal muscle differentiation. It belongs to a complex that includes C/EBPβ and PPARγ which are pivotal for the conversion of myoblasts into thermogenic brown fat cells. This activity contributes to the generation of cells specialized for heat production which aids in maintaining body temperature.
- Pathways
PRDM16 functions are linked to cellular metabolism and thermogenesis. It plays a substantial role in the Wnt/β-catenin signaling pathway where it influences energy balance. In this pathway PRDM16 interacts with Wnt signaling regulators and coactivators that control adipocyte differentiation and energy expenditure.
- Associated diseases and disorders
PRDM16 plays a role in myoblastic leukemia and obesity-related conditions. Changes in the function or expression of PRDM16 can contribute to pathological transformation leading to leukemia with proteins C/EBPβ and PPARγ also involved in related mechanisms. In obesity altered PRDM16 activity affects the formation and function of brown adipose tissue potentially disrupting normal energy metabolism and storage.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
Image is courtesy of a customer review submitted by Lena AppelsvedImmunocytochemistry - Anti-PRDM16 antibody (ab106410)
ab106410 staining RAP1 in Human Brown adipocytes from supraclavicular fat tissue by ICC (Immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 10% serum, 1% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (2 ug/ml in 10% serum, 1% BSA, 0.3% Triton X100, PBS) for 16 hours at 4°C. A Cy3®-conjugated Donkey F(ab')2 anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
Western blot - Anti-PRDM16 antibody (ab106410)
1 h incubation at RT in 5% NFDM/TBST.
All lanes: Western blot - Anti-PRDM16 antibody (ab106410) at 1 µg/mL
All lanes: MCF7 cell lysates at 15 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution
Observed band size: 130 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM16 antibody (ab106410)
Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-PRDM16 antibody (ab106410) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM16 antibody (ab106410)
Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-PRDM16 antibody (ab106410) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Western blot - Anti-PRDM16 antibody (ab106410)
1 h incubation at RT in 5% NFDM/TBST.
All lanes: Western blot - Anti-PRDM16 antibody (ab106410) at 2 µg/mL
All lanes: Rat spleen tissue lysate at 15 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution
Observed band size: 130 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM16 antibody (ab106410)
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PRDM16 antibody (ab106410) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM16 antibody (ab106410)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PRDM16 antibody (ab106410) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Western blot - Anti-PRDM16 antibody (ab106410)
1 h incubation at RT in 5% NFDM/TBST.
All lanes: Western blot - Anti-PRDM16 antibody (ab106410) at 2 µg/mL
All lanes: Mouse lung tissue lysate at 15 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution
Observed band size: 130 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com