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Knockout Tested Rabbit Recombinant Monoclonal PRDM16 antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 1 publication.

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Images

Western blot - Anti-PRDM16 antibody [EPR24315-59] (AB303534), expandable thumbnail
  • Western blot - Anti-PRDM16 antibody [EPR24315-59] (AB303534), expandable thumbnail
  • Western blot - Anti-PRDM16 antibody [EPR24315-59] (AB303534), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PIP
Human
Tested
Not recommended
Not recommended
Mouse
Tested
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Target data

Function

Binds DNA and functions as a transcriptional regulator (PubMed:18483224). Displays histone methyltransferase activity and monomethylates 'Lys-9' of histone H3 (H3K9me1) in vitro (PubMed:22939622). Probably catalyzes the monomethylation of free histone H3 in the cytoplasm which is then transported to the nucleus and incorporated into nucleosomes where SUV39H methyltransferases use it as a substrate to catalyze histone H3 'Lys-9' trimethylation (PubMed:22939622). Likely to be one of the primary histone methyltransferases along with MECOM/PRDM3 that direct cytoplasmic H3K9me1 methylation (PubMed:22939622). Functions in the differentiation of brown adipose tissue (BAT) which is specialized in dissipating chemical energy in the form of heat in response to cold or excess feeding while white adipose tissue (WAT) is specialized in the storage of excess energy and the control of systemic metabolism (PubMed:17618855, PubMed:18483224). Together with CEBPB, regulates the differentiation of myoblastic precursors into brown adipose cells (PubMed:18719582, PubMed:19641492). Functions as a repressor of TGF-beta signaling.

Alternative names

Kiaa1675, Mel1, Prdm16, Histone-lysine N-methyltransferase PRDM16, PR domain zinc finger protein 16, PR domain-containing protein 16, Transcription factor MEL1, MDS1/EVI1-like gene 1

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Knockout Tested Rabbit Recombinant Monoclonal PRDM16 antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR24315-59
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PRDM16 also known as the PR-domain containing 16 is a transcription cofactor involved in the regulation of genes. It has a mass of approximately 140 kDa. This protein is found in multiple tissues with high expression levels observed in brown adipose tissue and skeletal muscle. PRDM16 regulates gene expression by interacting with other transcription factors and modifying chromatin structure.

Biological function summary

PRDM16 is essential in the determination of brown adipose tissue and skeletal muscle differentiation. It belongs to a complex that includes C/EBPβ and PPARγ which are pivotal for the conversion of myoblasts into thermogenic brown fat cells. This activity contributes to the generation of cells specialized for heat production which aids in maintaining body temperature.

Pathways

PRDM16 functions are linked to cellular metabolism and thermogenesis. It plays a substantial role in the Wnt/β-catenin signaling pathway where it influences energy balance. In this pathway PRDM16 interacts with Wnt signaling regulators and coactivators that control adipocyte differentiation and energy expenditure.

Associated diseases and disorders

PRDM16 plays a role in myoblastic leukemia and obesity-related conditions. Changes in the function or expression of PRDM16 can contribute to pathological transformation leading to leukemia with proteins C/EBPβ and PPARγ also involved in related mechanisms. In obesity altered PRDM16 activity affects the formation and function of brown adipose tissue potentially disrupting normal energy metabolism and storage.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-PRDM16 antibody [EPR24315-59] (ab303534), expandable thumbnail

    Western blot - Anti-PRDM16 antibody [EPR24315-59] (ab303534)

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-PRDM16 antibody [EPR24315-59] (ab303534) at 1/1000 dilution

    Lane 1: HEK-293T cells transfected with an empty vector containing a myc-his tag, whole cell lysate at 20 µg

    Lane 2: HEK-293T cells transfected with a mouse PRDM16 expression vector containing a myc-his tag, whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 140 kDa

    Observed band size: 150 kDa, 170 kDa

    Exposure time: 1s

  • Western blot - Anti-PRDM16 antibody [EPR24315-59] (ab303534), expandable thumbnail

    Western blot - Anti-PRDM16 antibody [EPR24315-59] (ab303534)

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Low expression: kidney, skeletal muscle and liver (PMID: 17618855).

    The molecular weight observed is consistent with what has been described in the literature (PMID: 25662275).

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    Lysates were freshly made and used immediately to minimize protein degradation.

    All lanes: Western blot - Anti-PRDM16 antibody [EPR24315-59] (ab303534) at 1/1000 dilution

    Lane 1: Mouse brown fat tissue lysate at 20 µg

    Lane 2: Mouse kidney tissue lysate at 20 µg

    Lane 3: Mouse skeletal muscle tissue lysate at 20 µg

    Lane 4: Mouse liver tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 140 kDa

    Observed band size: 150 kDa, 170 kDa

    Exposure time: 180s

  • Western blot - Anti-PRDM16 antibody [EPR24315-59] (ab303534), expandable thumbnail

    Western blot - Anti-PRDM16 antibody [EPR24315-59] (ab303534)

    Western blot: Anti-PRDM16 antibody [EPR24315-59] (ab303534) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (Anti-Calnexin antibody [CANX/1543] ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab303534 was shown to bind specifically to PRDM16. A band was observed at 150 kDa in wild-type HCT 116 cell lysates with no signal observed at this size in PRDM16 knockout cell line. To generate this image, wild-type and PRDM16 knockout HCT 116 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-PRDM16 antibody [EPR24315-59] (ab303534) at 1/1000 dilution

    Lane 1: Wild-type HCT 116 cell lysate at 20 µg

    Lane 2: PRDM16 knockout HCT 116 cell lysate at 20 µg

    Lane 3: A549 cell lysate at 20 µg

    Lane 4: LnCap cell lysate at 20 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 150 kDa

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Product protocols

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