Rabbit Polyclonal PRDM4/PFM1 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PRDM4 aa 550-800.
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Publications
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- Oncogene 40:3318-33302021PRDM4 inhibits cell proliferation and tumorigenesis by inactivating the PI3K/AKT signaling pathway through targeting of PTEN in cervical carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWen-Ting Yang,Mei Chen,Rui Xu,Peng-Sheng ZhengPubMed 33846573
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human PRDM4 aa 550-800. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UKN5
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/500 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/100 - 1/500 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
May function as a transcription factor involved in cell differentiation.
Alternative names
PFM1, PRDM4, PR domain zinc finger protein 4, PR domain-containing protein 4
Recommended products
Rabbit Polyclonal PRDM4/PFM1 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PRDM4 aa 550-800.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human PRDM4 aa 550-800. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UKN5
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PRDM4 also known as PFM1 or PFM-1 is a member of the PR domain-containing protein family. It possesses an approximate molecular weight of 75 kDa. This protein functions primarily as a transcription factor with roles in the regulation of gene expression. PRDM4 localizes mainly in the nucleus where it influences transcriptional control. Expression of PRDM4/PFM1 has been observed in various tissues but shows significant levels in the brain and reproductive organs indicating its involvement in important biological processes in these regions.
- Biological function summary
PRDM4/PFM1 acts in cell differentiation and development. It is part of the KRAB-ZnF silencing complex which connects to other components to modify chromatin structure and regulate gene expression. This activity is important to maintaining cellular functions and contributes to cellular differentiation states. Understanding PRDM4/PFM1’s role within this complex can provide insights into its broader biological actions and its influence on cellular dynamics.
- Pathways
PRDM4/PFM1 plays a significant role in the TGF-beta and Hedgehog signaling pathways. These pathways are major determinants of cellular growth differentiation and apoptosis. Within these pathways PRDM4/PFM1 can associate with other proteins like SMAD family members and GLI transcription factors which are important in mediating cellular responses to external signals.
- Associated diseases and disorders
PRDM4/PFM1 links to neurodegenerative conditions and certain cancers. Its dysregulation has been noted in glioblastoma where an altered expression pattern suggests its involvement in tumor progression. PRDM4/PFM1 interacts with proteins involved in these disorders such as PTEN—a known tumor suppressor gene—highlighting its potential role in the development and progression of these diseases. Understanding these connections is key for developing therapeutic strategies targeting PRDM4/PFM1-related pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM4/PFM1 antibody (ab126939)
ab126939, at 1/500 dilution, staining PRDM4/PFM1 in Paraffin-embedded Human HBL435 xenograft tissue by Immunohistochemistry.
Western blot - Anti-PRDM4/PFM1 antibody (ab126939)
7.5% SDS PAGE
All lanes: Western blot - Anti-PRDM4/PFM1 antibody (ab126939) at 1/5000 dilution
Lane 1: PC3 whole cell lysate at 30 µg
Lane 2: U-87 MG whole cell lysate at 30 µg
Predicted band size: 88 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM4/PFM1 antibody (ab126939)
ab126939, at 1/500 dilution, staining PRDM4/PFM1 in Paraffin-embedded Mouse Muscle tissue by Immunohistochemistry.
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