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AB126939

Anti-PRDM4/PFM1 antibody

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(2 Publications)

Rabbit Polyclonal PRDM4/PFM1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PRDM4 aa 550-800.

View Alternative Names

PFM1, PRDM4, PR domain zinc finger protein 4, PR domain-containing protein 4

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-PRDM4/PFM1 antibody (AB126939)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PRDM4/PFM1 antibody (AB126939)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling PRDM4 protein at nucleus using ab126939 at a 1/1000 dilution (Green).
alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at a 1/1000 dilution (Red). Hoechst 33342 was used for nuclear staining (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM4/PFM1 antibody (AB126939)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM4/PFM1 antibody (AB126939)

ab126939, at 1/500 dilution, staining PRDM4/PFM1 in Paraffin-embedded Mouse Muscle tissue by Immunohistochemistry.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM4/PFM1 antibody (AB126939)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM4/PFM1 antibody (AB126939)

ab126939, at 1/500 dilution, staining PRDM4/PFM1 in Paraffin-embedded Human HBL435 xenograft tissue by Immunohistochemistry.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human PRDM4 aa 550-800. The exact immunogen used to generate this antibody is proprietary information.

Q9UKN5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PRDM4 also known as PFM1 or PFM-1 is a member of the PR domain-containing protein family. It possesses an approximate molecular weight of 75 kDa. This protein functions primarily as a transcription factor with roles in the regulation of gene expression. PRDM4 localizes mainly in the nucleus where it influences transcriptional control. Expression of PRDM4/PFM1 has been observed in various tissues but shows significant levels in the brain and reproductive organs indicating its involvement in important biological processes in these regions.
Biological function summary

PRDM4/PFM1 acts in cell differentiation and development. It is part of the KRAB-ZnF silencing complex which connects to other components to modify chromatin structure and regulate gene expression. This activity is important to maintaining cellular functions and contributes to cellular differentiation states. Understanding PRDM4/PFM1’s role within this complex can provide insights into its broader biological actions and its influence on cellular dynamics.

Pathways

PRDM4/PFM1 plays a significant role in the TGF-beta and Hedgehog signaling pathways. These pathways are major determinants of cellular growth differentiation and apoptosis. Within these pathways PRDM4/PFM1 can associate with other proteins like SMAD family members and GLI transcription factors which are important in mediating cellular responses to external signals.

PRDM4/PFM1 links to neurodegenerative conditions and certain cancers. Its dysregulation has been noted in glioblastoma where an altered expression pattern suggests its involvement in tumor progression. PRDM4/PFM1 interacts with proteins involved in these disorders such as PTEN—a known tumor suppressor gene—highlighting its potential role in the development and progression of these diseases. Understanding these connections is key for developing therapeutic strategies targeting PRDM4/PFM1-related pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May function as a transcription factor involved in cell differentiation.
See full target information PRDM4
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 11:22764 PubMed34815513

2021

Nanoplasmonic immunosensor for the detection of SCG2, a candidate serum biomarker for the early diagnosis of neurodevelopmental disorder.

Applications

Unspecified application

Species

Unspecified reactive species

So-Hee Lim,Yun-Ju Sung,Narae Jo,Na-Yoon Lee,Kyoung-Shim Kim,Da Yong Lee,Nam-Soon Kim,Jeehun Lee,Ju-Young Byun,Yong-Beom Shin,Jae-Ran Lee

Oncogene 40:3318-3330 PubMed33846573

2021

PRDM4 inhibits cell proliferation and tumorigenesis by inactivating the PI3K/AKT signaling pathway through targeting of PTEN in cervical carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Ting Yang,Mei Chen,Rui Xu,Peng-Sheng Zheng
View all publications
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Product promise

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