Rabbit Polyclonal PRDM9 antibody. Suitable for ICC, ELISA, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human PRDM9.
Constituents: Whole serum
| ICC | ELISA | WB | |
|---|---|---|---|
| Human | Expected | Expected | Expected |
| Mouse | Predicted | Predicted | Predicted |
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Histone methyltransferase that sequentially mono-, di-, and tri-methylates both 'Lys-4' (H3K4) and 'Lys-36' (H3K36) of histone H3 to produce respectively trimethylated 'Lys-4' (H3K4me3) and trimethylated 'Lys-36' (H3K36me3) histone H3 and plays a key role in meiotic prophase by determining hotspot localization thereby promoting meiotic recombination (PubMed:24095733, PubMed:24634223, PubMed:26833727, PubMed:27129774). Can also methylate all four core histones with H3 being the best substrate and the most highly modified (PubMed:24095733, PubMed:24634223, PubMed:26833727). Is also able, on one hand, to mono and di-methylate H4K20 and on other hand to trimethylate H3K9 with the di-methylated H3K9 as the best substrate (By similarity). During meiotic prophase, binds specific DNA sequences through its zinc finger domains thereby determining hotspot localization where it promotes local H3K4me3 and H3K36me3 enrichment on the same nucleosomes through its histone methyltransferase activity (PubMed:26833727). Thereby promotes double-stranded breaks (DSB) formation, at this subset of PRDM9-binding sites, that initiates meiotic recombination for the proper meiotic progression (By similarity). During meiotic progression hotspot-bound PRDM9 interacts with several complexes; in early leptonema binds CDYL and EHMT2 followed by EWSR1 and CXXC1 by the end of leptonema. EWSR1 joins PRDM9 with the chromosomal axis through REC8 (By similarity). In this way, controls the DSB repair pathway, pairing of homologous chromosomes and sex body formation (By similarity). Moreover plays a central role in the transcriptional activation of genes during early meiotic prophase thanks to H3K4me3 and H3K36me3 enrichment that represents a specific tag for epigenetic transcriptional activation (By similarity). In addition performs automethylation (By similarity). Acetylation and phosphorylation of histone H3 attenuate or prevent histone H3 methylation (By similarity).
PFM6, PRDM9, Histone-lysine N-methyltransferase PRDM9, PR domain zinc finger protein 9, PR domain-containing protein 9, Protein-lysine N-methyltransferase PRDM9, [histone H3]-lysine36 N-trimethyltransferase PRDM9, [histone H3]-lysine4 N-trimethyltransferase PRDM9, [histone H3]-lysine9 N-trimethyltransferase PRDM9, [histone H4]-N-methyl-L-lysine20 N-methyltransferase PRDM9, [histone H4]-lysine20 N-methyltransferase PRDM9
Rabbit Polyclonal PRDM9 antibody. Suitable for ICC, ELISA, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human PRDM9.
Constituents: Whole serum
ab101013 is predicted to crossreact with Human 56 kDa PRDM7.
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PRDM9 also known as Meisetz is a zinc finger protein that acts as a histone methyltransferase. This protein influences the organization and function of chromatin specifically by tri-methylating histone H3 at lysine 4 (H3K4me3). It weighs approximately 89 kDa. PRDM9 is mainly expressed in the reproductive organs where it plays a vital role in meiosis particularly during the early stages of gametogenesis. Its expression is important for initiating meiotic recombination hotspots.
PRDM9 plays a central role in germ cell development and meiosis. It initiates and regulates the recombination of genetic material during the production of gametes. PRDM9 operates as part of a complex with significant influence on the localization of DNA double-strand breaks thereby ensuring genetic diversity. It changes the chromatin structure around specific genomic regions to create an accessible environment for recombination machinery.
The protein plays an influential role in genetic recombination and chromatin modification pathways. In meiotic recombination PRDM9 determines the locations of recombination hotspots by binding to DNA and modifying adjacent histones. It functions alongside proteins such as SPO11 a topoisomerase-like protein which is essential for the initiation of homologous recombination through DNA double-strand breaks.
Mutations or aberrations in PRDM9 can lead to fertility issues and meiotic arrest. Variants of PRDM9 have been associated with azoospermia a condition characterized by a lack of sperm cells. Its interaction with other proteins like SPO11 links PRDM9 to developmental disorders related to faulty meiotic recombination processes highlighting its importance in reproductive health.
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