AB75815

Anti-Prealbumin antibody [EP2929Y]

Name: Anti-Prealbumin antibody [EP2929Y] (ab75815) | Abcam
Brand: Abcam Limited
SKU: AB75815
Price: 225 USD
Availability: InStock
Rating: 5 (5 reviews)

20ul selling size
RabMAb
Recombinant
What is this?

(5 Reviews)

(4 Publications)

Anti-Prealbumin antibody [EP2929Y] (ab75815) is a rabbit monoclonal antibody detecting Prealbumin in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency
- Trusted since 2009

View Alternative Names

PALB, TTR, Transthyretin, ATTR, Prealbumin, TBPA

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prealbumin antibody [EP2929Y] (AB75815)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prealbumin antibody [EP2929Y] (AB75815)

ab75815 at 1/250 dilution staining Prealbumin in human liver by Immunohistochemistry, Paraffin-embedded tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-Prealbumin antibody [EP2929Y] (AB75815)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Prealbumin antibody [EP2929Y] (AB75815)

Intracellular Flow Cytometry analysis of HepG2 cells labelling Prealbumin with ab75815 at a dilution of 1/50 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Green - Isotype control, rabbit monoclonal IgG.

Unknown

Western blot - Anti-Prealbumin antibody [EP2929Y] (AB75815)

All lanes:

Western blot - Anti-Prealbumin antibody [EP2929Y] (ab75815) at 1/5000 dilution

Lane 1:

human heart lysate at 10 µg

Lane 2:

human placenta lysate at 10 µg

Lane 3:

human brain lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 15 kDa

Observed band size: 16 kDa

false

Lab

Immunoprecipitation - Anti-Prealbumin antibody [EP2929Y] (AB75815)

Purified ab75815 at 1/50 dilution (2μg) immunoprecipitating Prealbumin in Human heart lysate.
Lane 1 (input) : Human heart lysate 10μg
Lane 2 (+) : ab75815 + Human heart lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab75815 in Human heart lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 16 kDa

All lanes:

Immunoprecipitation - Anti-Prealbumin antibody [EP2929Y] (ab75815)

Predicted band size: 15 kDa

false

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Prealbumin antibody [EP2929Y]
Carrier free

Anti-Prealbumin antibody [EP2929Y] - BSA and Azide free
578 PE

PE Anti-Prealbumin antibody [EP2929Y]
660 APC

APC Anti-Prealbumin antibody [EP2929Y]
HRP

HRP Anti-Prealbumin antibody [EP2929Y]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Prealbumin antibody [EP2929Y]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Prealbumin antibody [EP2929Y]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-Prealbumin antibody [EP2929Y]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Prealbumin antibody [EP2929Y]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Prealbumin antibody [EP2929Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2929Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), IP, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2500 - 1/5000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

Product details

What is this antibody validated in?
Anti-Prealbumin antibody [EP2929Y] (ab75815) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of Prealbumin?
Anti-Prealbumin [EP2929Y] (ab75815) specifically detects a band for Prealbumin (UniProt: P02766) at a molecular weight of 16kDa.

Reviewed by scientists
Anti-Prealbumin [EP2929Y] (ab75815) has over 5 independent reviews from customers.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EP2929Y] also available for your convenience: ab75815, Carrier free - ab185127, Alexa Fluor® 488 - ab199074, PE - ab305865, APC - ab305866, HRP - ab305867, Alkaline Phosphatase - ab308868, Alexa Fluor® 647 - ab310162, Alexa Fluor® 594 - ab310595, Alexa Fluor® 555 - ab312125, Alexa Fluor® 568 - ab312609, Alexa Fluor® 750 - ab321698

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prealbumin also known as transthyretin is a protein with a molecular mass of approximately 55 kDa. The liver primarily synthesizes prealbumin and it is present in the bloodstream. Functionally prealbumin transports thyroxine and retinol-binding protein-retinol complex facilitating the distribution of these molecules to target tissues. As such it plays an integral role in maintaining retinol and thyroid hormone homeostasis.

Biological function summary

Prealbumin interacts with retinol-binding protein to form a complex essential for vitamin A transportation. This complex prevents renal filtration of retinol-binding protein thereby conserving vitamin A in circulation. The protein's role extends beyond transport as it influences neurophysiological processes due to its ability to bind thyroid hormones and affect gene expression related to metabolism. Prealbumin is primarily a part of the thyroxine-transferring complex reinforcing its critical position in hormonal and vitamin dynamics.

Pathways

Prealbumin's role in the thyroxine-binding globulin pathway highlights its importance in the endocrine system. Another pathway where prealbumin contributes significantly is the vitamin A metabolic pathway. In these pathways prealbumin maintains functional relationships with proteins like retinol-binding protein and thyroxine-binding globulin which jointly modulate thyroid hormone transport and vitamin A dynamics across cellular membranes.

Prealbumin directly associates with familial amyloid polyneuropathy and malnutrition. Familial amyloid polyneuropathy involves the deposition of prealbumin-derived amyloid fibrils in peripheral nerves impacting neuron function. In malnutrition decreased prealbumin levels serve as a biomarker reflecting poor nutritional status. Clinically prealbumin's relation to retinol-binding protein and thyroid hormone levels facilitates its use in diagnosing and monitoring these conditions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.

See full target information TTR

style

left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Reproductive biology and endocrinology : RB&E 21:108 PubMed37968664

2023

Thyroid hormone transport and metabolism are disturbed in the placental villi of miscarriage.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Yu,Xinghao Feng,Zhongshan Lin,Xuan Li,Shiyue Su,Huiru Cheng,Yuanyuan Yang,Zhaolian Wei

Nature communications 12:7142 PubMed34880251

2021

Growth differentiation factor 1-induced tumour plasticity provides a therapeutic window for immunotherapy in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Cheng,Hao-Long Li,Shao-Yan Xi,Xiao-Feng Zhang,Yun Zhu, Le Xing,Yan-Xuan Mo,Mei-Mei Li,Fan-En Kong,Wen-Jie Zhu,Xiao-Gang Chen,Hui-Qing Cui,Zhi-Ming Cao,Yuan-Feng Gong,Yun-Qiang Tang,Yan Zhang,Xin-Yuan Guan,Ning-Fang Ma,Ming Liu

Oncology letters 19:3815-3826 PubMed32391095

2020

Identification of potential cervical cancer serum biomarkers in Thai patients.

Applications

Unspecified application

Species

Unspecified reactive species

Siriporn Keeratichamroen,Pantipa Subhasitanont,Daranee Chokchaichamnankit,Churat Weeraphan,Kittirat Saharat,Narongrit Sritana,Nuttavut Kantathavorn,Kriangpol Wiriyaukaradecha,Thaniya Sricharunrat,N Monique Paricharttanakul,Chirayu Auewarakul,Jisnuson Svasti,Chantragan Srisomsap

eLife 8: PubMed31393263

2019

Generation of human hepatic progenitor cells with regenerative and metabolic capacities from primary hepatocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Takeshi Katsuda,Juntaro Matsuzaki,Tomoko Yamaguchi,Yasuhiro Yamada,Marta Prieto-Vila,Kazunori Hosaka,Atsuko Takeuchi,Yoshimasa Saito,Takahiro Ochiya

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Prealbumin antibody [EP2929Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prealbumin antibody [EP2929Y] (AB75815)

Flow Cytometry (Intracellular) - Anti-Prealbumin antibody [EP2929Y] (AB75815)

Western blot - Anti-Prealbumin antibody [EP2929Y] (AB75815)

Immunoprecipitation - Anti-Prealbumin antibody [EP2929Y] (AB75815)

Related conjugates and formulations (10)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise