AB185127

Anti-Prealbumin antibody [EP2929Y] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal Prealbumin antibody. Carrier free. Suitable for IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

PALB, TTR, Transthyretin, ATTR, Prealbumin, TBPA

4 Images

Flow Cytometry (Intracellular) - Anti-Prealbumin antibody [EP2929Y] - BSA and Azide free (AB185127)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Prealbumin antibody [EP2929Y] - BSA and Azide free (AB185127)

Intracellular Flow Cytometry analysis of HepG2 cells labelling Prealbumin with ab75815 at a dilution of 1/50 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Green - Isotype control, rabbit monoclonal IgG.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75815).

Immunocytochemistry/ Immunofluorescence - Anti-Prealbumin antibody [EP2929Y] - BSA and Azide free (AB185127)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Prealbumin antibody [EP2929Y] - BSA and Azide free (AB185127)

Clone EP2929Y (ab185127) has been successfully conjugated by Abcam. This image was generated using Anti-Prealbumin antibody [EP2929Y] (Alexa Fluor® 488). Please refer to ab199074 for protocol details.

ab199074 staining Prealbumin in HepG2 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199074 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prealbumin antibody [EP2929Y] - BSA and Azide free (AB185127)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prealbumin antibody [EP2929Y] - BSA and Azide free (AB185127)

ab75815 at 1/250 dilution staining Prealbumin in human liver by Immunohistochemistry, Paraffin-embedded tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75815).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Lab

Immunoprecipitation - Anti-Prealbumin antibody [EP2929Y] - BSA and Azide free (AB185127)

This data was developed using ab75815, the same antibody clone in a different buffer formulation.
Purified ab75815 at 1/50 dilution (2μg) immunoprecipitating Prealbumin in Human heart lysate.
Lane 1 (input) : Human heart lysate 10μg
Lane 2 (+) : ab75815 + Human heart lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab75815 in Human heart lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 16 kDa

All lanes:

Immunoprecipitation - Anti-Prealbumin antibody [EP2929Y] (<a href='/en-us/products/primary-antibodies/prealbumin-antibody-ep2929y-ab75815'>ab75815</a>)

Predicted band size: 15 kDa

false

Unconjugated

Anti-Prealbumin antibody [EP2929Y]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Prealbumin antibody [EP2929Y]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Prealbumin antibody [EP2929Y]
578 PE

PE Anti-Prealbumin antibody [EP2929Y]
660 APC

APC Anti-Prealbumin antibody [EP2929Y]
HRP

HRP Anti-Prealbumin antibody [EP2929Y]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Prealbumin antibody [EP2929Y]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Prealbumin antibody [EP2929Y]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-Prealbumin antibody [EP2929Y]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Prealbumin antibody [EP2929Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2929Y

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, IP, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-low-endotoxin-azide-free-ab199376'>ab199376</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab185127 is the carrier-free version of ab75815.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prealbumin also known as transthyretin is a protein with a molecular mass of approximately 55 kDa. The liver primarily synthesizes prealbumin and it is present in the bloodstream. Functionally prealbumin transports thyroxine and retinol-binding protein-retinol complex facilitating the distribution of these molecules to target tissues. As such it plays an integral role in maintaining retinol and thyroid hormone homeostasis.

Biological function summary

Prealbumin interacts with retinol-binding protein to form a complex essential for vitamin A transportation. This complex prevents renal filtration of retinol-binding protein thereby conserving vitamin A in circulation. The protein's role extends beyond transport as it influences neurophysiological processes due to its ability to bind thyroid hormones and affect gene expression related to metabolism. Prealbumin is primarily a part of the thyroxine-transferring complex reinforcing its critical position in hormonal and vitamin dynamics.

Pathways

Prealbumin's role in the thyroxine-binding globulin pathway highlights its importance in the endocrine system. Another pathway where prealbumin contributes significantly is the vitamin A metabolic pathway. In these pathways prealbumin maintains functional relationships with proteins like retinol-binding protein and thyroxine-binding globulin which jointly modulate thyroid hormone transport and vitamin A dynamics across cellular membranes.

Prealbumin directly associates with familial amyloid polyneuropathy and malnutrition. Familial amyloid polyneuropathy involves the deposition of prealbumin-derived amyloid fibrils in peripheral nerves impacting neuron function. In malnutrition decreased prealbumin levels serve as a biomarker reflecting poor nutritional status. Clinically prealbumin's relation to retinol-binding protein and thyroid hormone levels facilitates its use in diagnosing and monitoring these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.

See full target information TTR

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 13:17292 PubMed37828232

2023

METTL3/IGF2BP2 axis affects the progression of colorectal cancer by regulating m6A modification of STAG3.

Applications

Unspecified application

Species

Unspecified reactive species

Jianmei Yi,Feng Peng,Jingli Zhao,Xiaosong Gong

Drug testing and analysis 14:80-91 PubMed34397154

2021

Transthyretin as a target of alkylation and a potential biomarker for sulfur mustard poisoning: Electrophoretic and mass spectrometric identification and characterization.

Applications

Unspecified application

Species

Unspecified reactive species

Wolfgang Schmeißer,Robin Lüling,Dirk Steinritz,Horst Thiermann,Theo Rein,Harald John

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Prealbumin antibody [EP2929Y] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-Prealbumin antibody [EP2929Y] - BSA and Azide free (AB185127)

Immunocytochemistry/ Immunofluorescence - Anti-Prealbumin antibody [EP2929Y] - BSA and Azide free (AB185127)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prealbumin antibody [EP2929Y] - BSA and Azide free (AB185127)

Immunoprecipitation - Anti-Prealbumin antibody [EP2929Y] - BSA and Azide free (AB185127)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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