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AB133284

Anti-Prealbumin antibody [EPR2928(2)]

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(1 Publication)

Rabbit Recombinant Monoclonal Prealbumin antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 1 publication.
3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prealbumin antibody [EPR2928(2)] (AB133284)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prealbumin antibody [EPR2928(2)] (AB133284)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling Prealbumin with ab133284 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-Prealbumin antibody [EPR2928(2)] (AB133284)
  • IP

Lab

Immunoprecipitation - Anti-Prealbumin antibody [EPR2928(2)] (AB133284)

Prealbumin was immunoprecipitated from 0.35 mg HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg with 133284 at 1/50 dilution (2μg). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg

Lane 2 : ab133284 IP in HeLa whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab133284 in HeLa whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Prealbumin antibody [EPR2928(2)] (ab133284)

Predicted band size: 15 kDa

Observed band size: 15 kDa

false

Western blot - Anti-Prealbumin antibody [EPR2928(2)] (AB133284)
  • WB

Unknown

Western blot - Anti-Prealbumin antibody [EPR2928(2)] (AB133284)

All lanes:

Western blot - Anti-Prealbumin antibody [EPR2928(2)] (ab133284) at 1/1000 dilution

Lane 1:

Human placenta lysate

Lane 2:

Human heart lysate

Lane 3:

Fetal kidney lysate

Lane 4:

Fetal liver lysate

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 15 kDa

false

  • Carrier free

    Anti-Prealbumin antibody [EPR2928(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2928(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prealbumin also known as transthyretin is a protein with a molecular mass of approximately 55 kDa. The liver primarily synthesizes prealbumin and it is present in the bloodstream. Functionally prealbumin transports thyroxine and retinol-binding protein-retinol complex facilitating the distribution of these molecules to target tissues. As such it plays an integral role in maintaining retinol and thyroid hormone homeostasis.
Biological function summary

Prealbumin interacts with retinol-binding protein to form a complex essential for vitamin A transportation. This complex prevents renal filtration of retinol-binding protein thereby conserving vitamin A in circulation. The protein's role extends beyond transport as it influences neurophysiological processes due to its ability to bind thyroid hormones and affect gene expression related to metabolism. Prealbumin is primarily a part of the thyroxine-transferring complex reinforcing its critical position in hormonal and vitamin dynamics.

Pathways

Prealbumin's role in the thyroxine-binding globulin pathway highlights its importance in the endocrine system. Another pathway where prealbumin contributes significantly is the vitamin A metabolic pathway. In these pathways prealbumin maintains functional relationships with proteins like retinol-binding protein and thyroxine-binding globulin which jointly modulate thyroid hormone transport and vitamin A dynamics across cellular membranes.

Prealbumin directly associates with familial amyloid polyneuropathy and malnutrition. Familial amyloid polyneuropathy involves the deposition of prealbumin-derived amyloid fibrils in peripheral nerves impacting neuron function. In malnutrition decreased prealbumin levels serve as a biomarker reflecting poor nutritional status. Clinically prealbumin's relation to retinol-binding protein and thyroid hormone levels facilitates its use in diagnosing and monitoring these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.
See full target information TTR

Alternative Names

PALB, TTR, Transthyretin, ATTR, Prealbumin, TBPA
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Publications (1)

Recent publications for all applications. Explore the full listand refine your search

The Journal of biological chemistry 301:110242 PubMed40381695

2025

Machine learning-based multimodal radiomics and transcriptomics models for predicting radiotherapy sensitivity and prognosis in esophageal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chengyu Ye,Hao Zhang,Zhou Chi,Zhina Xu,Yujie Cai,Yajing Xu,Xiangmin Tong
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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