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AB303503

Anti-Prealbumin (mutated V30M) antibody [EPR24341-111] - BSA and Azide free

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Rabbit Recombinant Monoclonal Prealbumin mutated V30M antibody. Carrier free. Suitable for Flow Cyt (Intra), WB and reacts with Transfected cell line - Human samples.

View Alternative Names

PALB, TTR, Transthyretin, ATTR, Prealbumin, TBPA

2 Images
Flow Cytometry (Intracellular) - Anti-Prealbumin (mutated V30M) antibody [EPR24341-111] - BSA and Azide free (AB303503)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Prealbumin (mutated V30M) antibody [EPR24341-111] - BSA and Azide free (AB303503)

This data was developed using ab303502, the same antibody clone in a different buffer formulation. Flow cytometric (Intracellular) analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293T (human embryonic kidney) cells transfected with a human Prealbumin expression vector containing a myc-his tag (Left panel) compared to HEK-293T transfected with a human Prealbumin (mutated V30M) expression vector containing a myc-his tag (Right panel) cells labeling Prealbumin (mutated V30M) with ab303502 at 1/500 dilution (0.1ug). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

Western blot - Anti-Prealbumin (mutated V30M) antibody [EPR24341-111] - BSA and Azide free (AB303503)
  • WB

Supplier Data

Western blot - Anti-Prealbumin (mutated V30M) antibody [EPR24341-111] - BSA and Azide free (AB303503)

This data was developed using ab303502, the same antibody clone in a different buffer formulation. Buffer/dilution concentration : 5% NFDM/TBST Exposure time : 3 minutes This blot was developed using ECL substrate

All lanes:

Western blot - Anti-Prealbumin (mutated V30M) antibody [EPR24341-111] (<a href='/en-us/products/primary-antibodies/prealbumin-mutated-v30m-antibody-epr24341-111-ab303502'>ab303502</a>) at 1/1000 dilution

Lane 1:

HEK-293T transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

HEK-293T transfected with human Prealbumin, containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 3:

HEK-293T transfected with human Prealbumin (mutated V30M), containing a myc-His-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 15 kDa

true

Exposure time: 3min

  • Unconjugated

    Anti-Prealbumin (mutated V30M) antibody [EPR24341-111]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24341-111

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Assay Kits

AB231920

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

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Primary Antibodies

AB32136

Anti-Amyloid Precursor Protein antibody [Y188]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amyloid Precursor Protein antibody [Y188] (AB32136)

  • 4
  • 9
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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prealbumin also known as transthyretin is a protein with a molecular mass of approximately 55 kDa. The liver primarily synthesizes prealbumin and it is present in the bloodstream. Functionally prealbumin transports thyroxine and retinol-binding protein-retinol complex facilitating the distribution of these molecules to target tissues. As such it plays an integral role in maintaining retinol and thyroid hormone homeostasis.
Biological function summary

Prealbumin interacts with retinol-binding protein to form a complex essential for vitamin A transportation. This complex prevents renal filtration of retinol-binding protein thereby conserving vitamin A in circulation. The protein's role extends beyond transport as it influences neurophysiological processes due to its ability to bind thyroid hormones and affect gene expression related to metabolism. Prealbumin is primarily a part of the thyroxine-transferring complex reinforcing its critical position in hormonal and vitamin dynamics.

Pathways

Prealbumin's role in the thyroxine-binding globulin pathway highlights its importance in the endocrine system. Another pathway where prealbumin contributes significantly is the vitamin A metabolic pathway. In these pathways prealbumin maintains functional relationships with proteins like retinol-binding protein and thyroxine-binding globulin which jointly modulate thyroid hormone transport and vitamin A dynamics across cellular membranes.

Prealbumin directly associates with familial amyloid polyneuropathy and malnutrition. Familial amyloid polyneuropathy involves the deposition of prealbumin-derived amyloid fibrils in peripheral nerves impacting neuron function. In malnutrition decreased prealbumin levels serve as a biomarker reflecting poor nutritional status. Clinically prealbumin's relation to retinol-binding protein and thyroid hormone levels facilitates its use in diagnosing and monitoring these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.
See full target information TTR mutated V30M
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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