AB181212

Anti-PREB antibody [EPR13252]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal PREB antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

SEC12, PREB, Guanine nucleotide-exchange factor SEC12, Prolactin regulatory element-binding protein

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-PREB antibody [EPR13252] (AB181212)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PREB antibody [EPR13252] (AB181212)

Immunofluorescent analysis of HT-1376 cells (paraformaldehyde-fixed 4%) labeling PREB with ab181212 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PREB antibody [EPR13252] (AB181212)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PREB antibody [EPR13252] (AB181212)

Immunohistochemical analysis of paraffin-embedded Human endometrial adenocarcinoma tissue labeling PREB with ab181212 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PREB antibody [EPR13252] (AB181212)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PREB with ab181212 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-PREB antibody [EPR13252] (AB181212)

Western blot analysis of immunoprecipitation pellet from Raji cell lysate (lane 1) or a negative control (lane 2) immunoprecipitated using ab181212 at 1/50 dilution.
Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-PREB antibody [EPR13252] (ab181212)

Predicted band size: 45 kDa

false

Supplier Data

Western blot - Anti-PREB antibody [EPR13252] (AB181212)

All lanes:

Western blot - Anti-PREB antibody [EPR13252] (ab181212) at 1/5000 dilution

Lane 1:

HT-1376 cell lysate at 10 µg

Lane 2:

Raji cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 45 kDa

false

Carrier free

Anti-PREB antibody [EPR13252] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13252

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30 - 1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PREB also known as prolactin regulatory element-binding protein weighs approximately 41 kDa. It acts as a transcription factor that binds to specific DNA sequences to regulate gene expression. Researchers find it expressed in various tissues including the pituitary gland liver and pancreas. Its activity involves the control of prolactin transcription a hormone important for lactation and possibly other genes with related promoter elements.

Biological function summary

PREB regulates gene expression by interacting with mitogenic signaling pathways. As part of a protein complex it influences cellular proliferation and secretion processes. Its transcriptional regulation mechanism allows cells to respond to physiological changes both at developmental stages and in adult organisms. This regulation plays a role in maintaining normal cellular functions indicating its importance in various biological processes.

Pathways

PREB impacts the MAPK/ERK signaling pathway and the pancreatic secretion pathway. These pathways are significant for cellular growth differentiation and secretion. Furthermore PREB interacts with proteins like prolactin and ERK contributing to the regulation of transcriptional responses necessary for effective signal processing. These interactions highlight the involvement of PREB in essential physiological and cellular processes.

PREB relates to conditions such as breast cancer and type 2 diabetes. Its dysregulation has been associated with abnormal cell proliferation seen in breast cancer where it may interact with proteins like estrogen receptor. Furthermore PREB influences insulin regulation in the pancreas linking it to type 2 diabetes. By modulating gene expression it directly or indirectly contributes to the progression or prevention of these disorders.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Guanine nucleotide exchange factor (GEF) that regulates the assembly of the coat protein complex II/COPII in endoplasmic reticulum (ER) to Golgi vesicle-mediated transport. Selectively activates SAR1A and SAR1B by promoting the exchange of guanosine diphosphate (GDP) for guanosine triphosphate (GTP) in these small GTPases (PubMed : 32358066). In their activated GTP-bound state, SAR1A and SAR1B insert into the membrane of the endoplasmic reticulum where they recruit the remainder of the coat protein complex II/COPII which is responsible for both the sorting of proteins and the deformation and budding of membranes into vesicles destined to the Golgi (PubMed : 32358066).. Was first identified based on its probable role in the regulation of pituitary gene transcription. Binds to the prolactin gene (PRL) promoter and seems to activate transcription.

See full target information PREB

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:7421 PubMed39198433

2024

IGF2BP3 promotes mRNA degradation through internal mG modification.

Applications

Unspecified application

Species

Unspecified reactive species

Chang Liu,Xiaoyang Dou,Yutao Zhao,Linda Zhang,Lisheng Zhang,Qing Dai,Jun Liu,Tong Wu,Yu Xiao,Chuan He

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PREB antibody [EPR13252]

Immunocytochemistry/ Immunofluorescence - Anti-PREB antibody [EPR13252] (AB181212)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PREB antibody [EPR13252] (AB181212)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PREB antibody [EPR13252] (AB181212)

Immunoprecipitation - Anti-PREB antibody [EPR13252] (AB181212)

Western blot - Anti-PREB antibody [EPR13252] (AB181212)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise