Rabbit Recombinant Monoclonal PREB antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Human samples.
Images
![Immunoprecipitation - Anti-PREB antibody [EPR13252] - BSA and Azide free (AB250431), expandable thumbnail](https://content.abcam.com/products/images/preb-antibody-epr13252-bsa-and-azide-free-ab250431--immunoprecipitation-img46497.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-PREB antibody [EPR13252] - BSA and Azide free (AB250431)")
![Western blot - Anti-PREB antibody [EPR13252] - BSA and Azide free (AB250431), expandable thumbnail](https://content.abcam.com/products/images/preb-antibody-epr13252-bsa-and-azide-free-ab250431--western-blot-img145809.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PREB antibody [EPR13252] - BSA and Azide free (AB250431)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Function
Guanine nucleotide exchange factor (GEF) that regulates the assembly of the coat protein complex II/COPII in endoplasmic reticulum (ER) to Golgi vesicle-mediated transport. Selectively activates SAR1A and SAR1B by promoting the exchange of guanosine diphosphate (GDP) for guanosine triphosphate (GTP) in these small GTPases (PubMed:32358066). In their activated GTP-bound state, SAR1A and SAR1B insert into the membrane of the endoplasmic reticulum where they recruit the remainder of the coat protein complex II/COPII which is responsible for both the sorting of proteins and the deformation and budding of membranes into vesicles destined to the Golgi (PubMed:32358066). Was first identified based on its probable role in the regulation of pituitary gene transcription. Binds to the prolactin gene (PRL) promoter and seems to activate transcription.
Alternative names
SEC12, PREB, Guanine nucleotide-exchange factor SEC12, Prolactin regulatory element-binding protein
Recommended products
Rabbit Recombinant Monoclonal PREB antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR13252
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab250431 is the carrier-free version of Anti-PREB antibody [EPR13252] ab181212.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PREB also known as prolactin regulatory element-binding protein weighs approximately 41 kDa. It acts as a transcription factor that binds to specific DNA sequences to regulate gene expression. Researchers find it expressed in various tissues including the pituitary gland liver and pancreas. Its activity involves the control of prolactin transcription a hormone important for lactation and possibly other genes with related promoter elements.
- Biological function summary
PREB regulates gene expression by interacting with mitogenic signaling pathways. As part of a protein complex it influences cellular proliferation and secretion processes. Its transcriptional regulation mechanism allows cells to respond to physiological changes both at developmental stages and in adult organisms. This regulation plays a role in maintaining normal cellular functions indicating its importance in various biological processes.
- Pathways
PREB impacts the MAPK/ERK signaling pathway and the pancreatic secretion pathway. These pathways are significant for cellular growth differentiation and secretion. Furthermore PREB interacts with proteins like prolactin and ERK contributing to the regulation of transcriptional responses necessary for effective signal processing. These interactions highlight the involvement of PREB in essential physiological and cellular processes.
- Associated diseases and disorders
PREB relates to conditions such as breast cancer and type 2 diabetes. Its dysregulation has been associated with abnormal cell proliferation seen in breast cancer where it may interact with proteins like estrogen receptor. Furthermore PREB influences insulin regulation in the pancreas linking it to type 2 diabetes. By modulating gene expression it directly or indirectly contributes to the progression or prevention of these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunoprecipitation - Anti-PREB antibody [EPR13252] - BSA and Azide free (ab250431), expandable thumbnail](https://content.abcam.com/products/images/preb-antibody-epr13252-bsa-and-azide-free-ab250431--immunoprecipitation-img46497.jpg "Immunoprecipitation - Anti-PREB antibody [EPR13252] - BSA and Azide free (ab250431)")
Immunoprecipitation - Anti-PREB antibody [EPR13252] - BSA and Azide free (ab250431)
This data was developed using Anti-PREB antibody [EPR13252] ab181212, the same antibody clone in a different buffer formulation.Western blot analysis of immunoprecipitation pellet from Raji cell lysate (lane 1) or a negative control (lane 2) immunoprecipitated using Anti-PREB antibody [EPR13252] ab181212 at 1/50 dilution.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.All lanes: Immunoprecipitation - Anti-PREB antibody [EPR13252] (Anti-PREB antibody [EPR13252] ab181212)
Predicted band size: 45 kDa
![Western blot - Anti-PREB antibody [EPR13252] - BSA and Azide free (ab250431), expandable thumbnail](https://content.abcam.com/products/images/preb-antibody-epr13252-bsa-and-azide-free-ab250431--western-blot-img145809.jpg "Western blot - Anti-PREB antibody [EPR13252] - BSA and Azide free (ab250431)")
Western blot - Anti-PREB antibody [EPR13252] - BSA and Azide free (ab250431)
This data was developed using Anti-PREB antibody [EPR13252] ab181212, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-PREB antibody [EPR13252] (Anti-PREB antibody [EPR13252] ab181212) at 1/5000 dilution
Lane 1: HT-1376 cell lysate at 10 µg
Lane 2: Raji cell lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 45 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-PREB antibody [EPR13252] - BSA and Azide free (ab250431), expandable thumbnail](https://content.abcam.com/products/images/preb-antibody-epr13252-bsa-and-azide-free-ab250431--immunocytochemistry-immunofluorescence-img70599.gif "Immunocytochemistry/ Immunofluorescence - Anti-PREB antibody [EPR13252] - BSA and Azide free (ab250431)")
Immunocytochemistry/ Immunofluorescence - Anti-PREB antibody [EPR13252] - BSA and Azide free (ab250431)
This data was developed using Anti-PREB antibody [EPR13252] ab181212, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HT-1376 cells (paraformaldehyde-fixed, 4%) labeling PREB with Anti-PREB antibody [EPR13252] ab181212 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PREB antibody [EPR13252] - BSA and Azide free (ab250431), expandable thumbnail](https://content.abcam.com/products/images/preb-antibody-epr13252-bsa-and-azide-free-ab250431--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img138440.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PREB antibody [EPR13252] - BSA and Azide free (ab250431)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PREB antibody [EPR13252] - BSA and Azide free (ab250431)
This data was developed using Anti-PREB antibody [EPR13252] ab181212, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human endometrial adenocarcinoma tissue labeling PREB with Anti-PREB antibody [EPR13252] ab181212 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PREB antibody [EPR13252] - BSA and Azide free (ab250431), expandable thumbnail](https://content.abcam.com/products/images/preb-antibody-epr13252-bsa-and-azide-free-ab250431--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136965.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PREB antibody [EPR13252] - BSA and Azide free (ab250431)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PREB antibody [EPR13252] - BSA and Azide free (ab250431)
This data was developed using Anti-PREB antibody [EPR13252] ab181212, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PREB with Anti-PREB antibody [EPR13252] ab181212 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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