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AB250431

Anti-PREB antibody [EPR13252] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal PREB antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

SEC12, PREB, Guanine nucleotide-exchange factor SEC12, Prolactin regulatory element-binding protein

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-PREB antibody [EPR13252] - BSA and Azide free (AB250431)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PREB antibody [EPR13252] - BSA and Azide free (AB250431)

This data was developed using ab181212, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of HT-1376 cells (paraformaldehyde-fixed, 4%) labeling PREB with ab181212 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PREB antibody [EPR13252] - BSA and Azide free (AB250431)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PREB antibody [EPR13252] - BSA and Azide free (AB250431)

This data was developed using ab181212, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human endometrial adenocarcinoma tissue labeling PREB with ab181212 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PREB antibody [EPR13252] - BSA and Azide free (AB250431)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PREB antibody [EPR13252] - BSA and Azide free (AB250431)

This data was developed using ab181212, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PREB with ab181212 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-PREB antibody [EPR13252] - BSA and Azide free (AB250431)
  • IP

Supplier Data

Immunoprecipitation - Anti-PREB antibody [EPR13252] - BSA and Azide free (AB250431)

This data was developed using ab181212, the same antibody clone in a different buffer formulation.

Western blot analysis of immunoprecipitation pellet from Raji cell lysate (lane 1) or a negative control (lane 2) immunoprecipitated using ab181212 at 1/50 dilution.
Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-PREB antibody [EPR13252] (<a href='/en-us/products/primary-antibodies/preb-antibody-epr13252-ab181212'>ab181212</a>)

Predicted band size: 45 kDa

false

Western blot - Anti-PREB antibody [EPR13252] - BSA and Azide free (AB250431)
  • WB

Supplier Data

Western blot - Anti-PREB antibody [EPR13252] - BSA and Azide free (AB250431)

This data was developed using ab181212, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PREB antibody [EPR13252] (<a href='/en-us/products/primary-antibodies/preb-antibody-epr13252-ab181212'>ab181212</a>) at 1/5000 dilution

Lane 1:

HT-1376 cell lysate at 10 µg

Lane 2:

Raji cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 45 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13252

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, IHC-P, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab250431 is the carrier-free version of ab181212.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PREB also known as prolactin regulatory element-binding protein weighs approximately 41 kDa. It acts as a transcription factor that binds to specific DNA sequences to regulate gene expression. Researchers find it expressed in various tissues including the pituitary gland liver and pancreas. Its activity involves the control of prolactin transcription a hormone important for lactation and possibly other genes with related promoter elements.
Biological function summary

PREB regulates gene expression by interacting with mitogenic signaling pathways. As part of a protein complex it influences cellular proliferation and secretion processes. Its transcriptional regulation mechanism allows cells to respond to physiological changes both at developmental stages and in adult organisms. This regulation plays a role in maintaining normal cellular functions indicating its importance in various biological processes.

Pathways

PREB impacts the MAPK/ERK signaling pathway and the pancreatic secretion pathway. These pathways are significant for cellular growth differentiation and secretion. Furthermore PREB interacts with proteins like prolactin and ERK contributing to the regulation of transcriptional responses necessary for effective signal processing. These interactions highlight the involvement of PREB in essential physiological and cellular processes.

PREB relates to conditions such as breast cancer and type 2 diabetes. Its dysregulation has been associated with abnormal cell proliferation seen in breast cancer where it may interact with proteins like estrogen receptor. Furthermore PREB influences insulin regulation in the pancreas linking it to type 2 diabetes. By modulating gene expression it directly or indirectly contributes to the progression or prevention of these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Guanine nucleotide exchange factor (GEF) that regulates the assembly of the coat protein complex II/COPII in endoplasmic reticulum (ER) to Golgi vesicle-mediated transport. Selectively activates SAR1A and SAR1B by promoting the exchange of guanosine diphosphate (GDP) for guanosine triphosphate (GTP) in these small GTPases (PubMed : 32358066). In their activated GTP-bound state, SAR1A and SAR1B insert into the membrane of the endoplasmic reticulum where they recruit the remainder of the coat protein complex II/COPII which is responsible for both the sorting of proteins and the deformation and budding of membranes into vesicles destined to the Golgi (PubMed : 32358066).. Was first identified based on its probable role in the regulation of pituitary gene transcription. Binds to the prolactin gene (PRL) promoter and seems to activate transcription.
See full target information PREB
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medicine : eCAM 2022:7757833 PubMed36091585

2022

Esculentoside A Alleviates Intestinal Dysmotility in Ulcerative Colitis by Regulating HS/CSE and NO/nNOS Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Wenhua Wei,Shiwei Liang,Haicheng Fang,Jie Cao
View all publications
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Product promise

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