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AB154587

Anti-PREP1 antibody

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(2 Publications)

Rabbit Polyclonal PREP1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PKNOX1 aa 200 to C-terminus.

View Alternative Names

PREP1, PKNOX1, Homeobox protein PKNOX1, Homeobox protein PREP-1, PBX/knotted homeobox 1

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-PREP1 antibody (AB154587)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PREP1 antibody (AB154587)

HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. They were stained using ab154587 diluted at 1/1000 (green).
Nuclear staining Hoechst 33342 staining (blue).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human PKNOX1 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P55347

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Keep as concentrated solution.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PREP1 also called PBX/knotted 1 homeobox 1 (PKNOX1) is a transcription factor. It weighs approximately 45 kDa. Expression of PREP1 is mainly found in the nucleus of various cell types particularly in tissues with rapid cell proliferation like embryonic development regions spleen and thymus. PREP1 is part of the homeobox gene family and is involved in regulating genes necessary for cellular differentiation and growth.
Biological function summary

The function of PREP1 involves the regulation of numerous developmental processes. It interacts with proteins to form heterodimeric complexes like the PREP1-PBX complex which modulate the transcription of specific target genes. This regulation is important for proper development during embryogenesis and also contributes to hematopoietic cell differentiation. Through these mechanisms PREP1 influences processes underlying organogenesis and cellular identity.

Pathways

The function of PREP1 integrates into the broader context of key regulatory cascades including the Hox gene network and the insulin-signaling pathway. PREP1 interacts with PBX proteins within these pathways affecting the transcriptional programs they control. In particular by influencing the Hox pathway PREP1 plays a role in the spatial patterning of body structures and anterior-posterior axis formation in the embryo. This regulatory activity highlights its importance in the intricate orchestration of developmental cues.

Mutations or dysregulation of PREP1 impact conditions like cancer and diabetes. Abnormal PREP1 expression has been connected to tumorigenesis in organs where its developmental control is critical. For example studies show altered PREP1 levels in certain leukemias with implications for progression and outcome. In diabetes the interplay between PREP1 and insulin-signaling pathways can affect glucose metabolism and pancreatic function linking PREP1 to metabolic disorders where it may cooperate with proteins like FOXO1 in influencing glucose homeostasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Activates transcription in the presence of PBX1A and HOXA1.
See full target information PKNOX1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of immunopathology and pharmacology 37:3946320231208833 PubMed37864517

2023

PKNOX1 acts as a transcription factor of DHH and promotes the progression of stomach adenocarcinoma by regulating the Hedgehog signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Liu,Siwei Zhao,Liqing Liu

Stem cells international 2022:5609501 PubMed36277041

2022

MFG-E8 Exerts Neuroprotection in Neural Stem Cells Induced by Anesthetic Sevoflurane via Regulating the PI3K/AKT Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Minmin Cai,Liufang Sheng
View all publications
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