Anti-Presenilin 1/PS-1 antibody [APS 18]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Presenilin 1/PS-1 antibody [APS 18] (AB15458)

Immunohistochemistry was performed on normal biopsies of deparaffinized Human tonsil tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1 : 20 with a mouse monoclonal antibody recognizing Presenilin 1 / PS-1 ab15458 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Presenilin 1/PS-1 antibody [APS 18] (AB15458)

Immunofluorescent analysis of Presenilin 1 / PS-1 using Presenilin 1 / PS-1 Monoclonal antibody (APS 18) ab115458 shows staining in A2058 melanoma cells. Presenilin 1 / PS-1 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Presenilin 1 / PS-1 ab115458 at a dilution of 1 : 20-1 : 100 over night at 4 °C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Presenilin 1/PS-1 antibody [APS 18] (AB15458)

Immunofluorescent analysis of Presenilin 1 / PS-1 using Presenilin 1 / PS-1 Monoclonal antibody (APS 18) ab115458 shows staining in HeLa cells. Presenilin 1 / PS-1 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Presenilin 1 / PS-1 ab115458 at a dilution of 1 : 20-1 : 100 over night at 4 °C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Presenilin 1/PS-1 antibody [APS 18] (AB15458)

Immunofluorescent analysis of Presenilin 1 / PS-1 using Presenilin 1 / PS-1 Monoclonal antibody (APS 18) ab115458 shows staining in MCF-7 cells. Presenilin 1 / PS-1 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Presenilin 1 / PS-1 ab115458 at a dilution of 1 : 20-1 : 100 over night at 4 °C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Presenilin 1/PS-1 antibody [APS 18] (AB15458)

Immunohistochemistry was performed on normal biopsies of deparaffinized Human liver tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1 : 200 with a mouse monoclonal antibody recognizing Presenilin 1 / PS-1 ab15458 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Presenilin 1/PS-1 antibody [APS 18] (AB15458)

IF showing PS1 in mouse fibroblasts using ab15458.

• WB

Supplier Data

Western blot - Anti-Presenilin 1/PS-1 antibody [APS 18] (AB15458)

All lanes:

Western blot - Anti-Presenilin 1/PS-1 antibody [APS 18] (ab15458) at 1/500 dilution

Lane 1:

T-47D (human ductal breast epithelial tumor cell line) whole cell lysate at 30 µg

Lane 2:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 30 µg

Lane 3:

Daudi (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg

Lane 4:

SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate at 30 µg

Lane 5:

Caco-2 (human colorectal adenocarcinoma cell line) whole cell lysate at 30 µg

Secondary

All lanes:

Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution

Predicted band size: 53 kDa

false