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AB76131

Anti-Presenilin 1/PS-1 (phospho S310) antibody [EP2001Y]

4

(1 Review)

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(1 Publication)

Rabbit Recombinant Monoclonal Presenilin 1/PS-1 phospho S310 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

AD3, PS1, PSNL1, PSEN1, Presenilin-1, PS-1, Protein S182

1 Images
Western blot - Anti-Presenilin 1/PS-1 (phospho S310) antibody [EP2001Y] (AB76131)
  • WB

Supplier Data

Western blot - Anti-Presenilin 1/PS-1 (phospho S310) antibody [EP2001Y] (AB76131)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Primary antibody : incubated for 1h at room temperature. Secondary antibody : incubated for 1h at room temperature. ab181602 was used as a loading control.

All lanes:

Western blot - Anti-Presenilin 1/PS-1 (phospho S310) antibody [EP2001Y] (ab76131) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell), starve overnight, whole cell lysate at 15 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell), starve overnight, treated with 200nM TPA for 4 hours whole cell lysate at 15 µg

Lane 3:

HeLa (Human cervix adenocarcinoma epithelial cell), starve overnight, treated with 200nM TPA for 4 hours. Then the membrane treated with Alkaline phosphatase at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 20 kDa

false

Exposure time: 40s

  • Carrier free

    Anti-Presenilin 1/PS-1 (phospho S310) antibody [EP2001Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2001Y

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Presenilin 1 often referred to as PS-1 is a protein with an important role in the function of gamma-secretase a multi-subunit protease complex. It weighs approximately 50 kDa and is widely expressed in different tissues especially in the brain. Presenilin 1 functions as part of the gamma-secretase complex which is responsible for processing several type I transmembrane proteins. This protein undergoes endoproteolytic processing to form an N-terminal and C-terminal fragment which are pivotal for its gamma-secretase activity.
Biological function summary

Presenilin 1 plays a fundamental role in the cleavage of amyloid precursor protein (APP) within the transmembrane domain. This cleavage results in the formation of amyloid-beta peptides which are of significant interest in neurobiology. Presenilin 1 is an integral component of the gamma-secretase complex that includes nicastrin APH-1 and PEN-2. This complex involvement underlines the enzyme's importance in cell signaling pathways and modulation of synaptic functions. The functionality at the synaptic level is particularly important given its expression pattern and involvement in neural processes.

Pathways

The action of presenilin 1 is closely linked to the Notch signaling pathway and the amyloidogenic pathway. Presenilin 1 facilitates the intramembrane cleavage of the Notch receptor releasing the Notch intracellular domain that translocates to the nucleus and affects gene transcription. The protein's role in processing APP into amyloid-beta peptides links it directly to Alzheimer's disease pathology. The protein Tax also a component of the gamma-secretase complex shows interdependencies in these pathways.

Presenilin 1 holds significant relevance in Alzheimer's disease where mutations in its gene are associated with early-onset familial Alzheimer's. The production of amyloid-beta peptides through presenilin 1's interaction with APP is central to the disease's pathogenesis. Another disorder linked to presenilin 1 is Dilated Cardiomyopathy where presenilin mutations may affect calcium signaling in cardiac cells. In Alzheimer's the interaction between presenilin 1 and the amyloid-beta peptide protein is critical while in cardiac issues it's more about protein misfolding and signaling mishaps.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein) (PubMed : 10206644, PubMed : 10545183, PubMed : 10593990, PubMed : 10811883, PubMed : 10899933, PubMed : 12679784, PubMed : 12740439, PubMed : 15274632, PubMed : 20460383, PubMed : 25043039, PubMed : 26280335, PubMed : 28269784, PubMed : 30598546, PubMed : 30630874). Requires the presence of the other members of the gamma-secretase complex for protease activity (PubMed : 15274632, PubMed : 25043039, PubMed : 26280335, PubMed : 30598546, PubMed : 30630874). Plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels (PubMed : 10593990, PubMed : 10811883, PubMed : 10899933, PubMed : 9738936). Stimulates cell-cell adhesion via its interaction with CDH1; this stabilizes the complexes between CDH1 (E-cadherin) and its interaction partners CTNNB1 (beta-catenin), CTNND1 and JUP (gamma-catenin) (PubMed : 11953314). Under conditions of apoptosis or calcium influx, cleaves CDH1 (PubMed : 11953314). This promotes the disassembly of the complexes between CDH1 and CTNND1, JUP and CTNNB1, increases the pool of cytoplasmic CTNNB1, and thereby negatively regulates Wnt signaling (PubMed : 11953314, PubMed : 9738936). Required for normal embryonic brain and skeleton development, and for normal angiogenesis (By similarity). Mediates the proteolytic cleavage of EphB2/CTF1 into EphB2/CTF2 (PubMed : 17428795, PubMed : 28269784). The holoprotein functions as a calcium-leak channel that allows the passive movement of calcium from endoplasmic reticulum to cytosol and is therefore involved in calcium homeostasis (PubMed : 16959576, PubMed : 25394380). Involved in the regulation of neurite outgrowth (PubMed : 15004326, PubMed : 20460383). Is a regulator of presynaptic facilitation, spike transmission and synaptic vesicles replenishment in a process that depends on gamma-secretase activity. It acts through the control of SYT7 presynaptic expression (By similarity).
See full target information PSEN1 phospho S310
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 12:3242-3248 PubMed27882144

2016

Effect of cyclosporin A intervention on the immunological mechanisms of coronary heart disease and restenosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Wang,Yue-Cheng Hu,Ru-Yan Zhang,Dong-Xia Jin,Yuan Jiang,He-Nan Zhang,Hong-Liang Cong
View all publications
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Product promise

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