Rabbit Monoclonal Presenilin 2/AD5 phospho S327 antibody. Carrier free. Suitable for Dot, WB and reacts with Human samples.
Images
![Western blot - Anti-Presenilin 2/AD5 (phospho S327) antibody [EP2613Y] - BSA and Azide free (AB284741), expandable thumbnail](https://content.abcam.com/products/images/presenilin-2-ad5-phospho-s327-antibody-ep2613y-bsa-and-azide-free-ab284741--western-blot-img422021.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Presenilin 2/AD5 (phospho S327) antibody [EP2613Y] - BSA and Azide free (AB284741)")
![Dot Blot - Anti-Presenilin 2/AD5 (phospho S327) antibody [EP2613Y] - BSA and Azide free (AB284741), expandable thumbnail](https://content.abcam.com/products/images/presenilin-2-ad5-phospho-s327-antibody-ep2613y-bsa-and-azide-free-ab284741--dot-blot-img422019.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-Presenilin 2/AD5 (phospho S327) antibody [EP2613Y] - BSA and Azide free (AB284741)")
![Western blot - Anti-Presenilin 2/AD5 (phospho S327) antibody [EP2613Y] - BSA and Azide free (AB284741), expandable thumbnail](https://content.abcam.com/products/images/presenilin-2-ad5-phospho-s327-antibody-ep2613y-bsa-and-azide-free-ab284741--western-blot-img422020.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Presenilin 2/AD5 (phospho S327) antibody [EP2613Y] - BSA and Azide free (AB284741)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Dot | WB | |
|---|---|---|
| Human | Tested | Tested |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein). Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. May function in the cytoplasmic partitioning of proteins. The holoprotein functions as a calcium-leak channel that allows the passive movement of calcium from endoplasmic reticulum to cytosol and is involved in calcium homeostasis (PubMed:16959576). Is a regulator of mitochondrion-endoplasmic reticulum membrane tethering and modulates calcium ions shuttling between ER and mitochondria (PubMed:21285369).
Alternative names
AD4, PS2, PSNL2, STM2, PSEN2, Presenilin-2, PS-2, AD3LP, AD5, E5-1, STM-2
Recommended products
Rabbit Monoclonal Presenilin 2/AD5 phospho S327 antibody. Carrier free. Suitable for Dot, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EP2613Y
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab284741 is the carrier-free version of Anti-Presenilin 2/AD5 (phospho S327) antibody [EP2613Y] ab76114
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The presenilin 2 (PSEN2) protein also known as AD5 plays a role as a catalytic subunit in the gamma-secretase complex. This complex is involved in intramembrane proteolysis of several type-I transmembrane proteins. PSEN2 has a mass of about 52 kDa and is expressed mainly in the brain and several other tissues throughout the body. Its gene is located on chromosome 1 and has the alternative name 'EP-2614'. Presenilin 2 is closely associated with presenilin 1 (PSEN1) a related protein within the same family.
- Biological function summary
Presenilin 2 functions in the regulation of proteolytic processing of the amyloid precursor protein (APP) within the gamma-secretase complex. This action produces amyloid beta peptides which are critical for normal brain function but may accumulate under pathological conditions. The protein is also involved in NOTCH receptor activation which plays a part in cell fate determination during development. The gamma-secretase complex includes other key proteins such as nicastrin anterior pharynx-defective 1 (APH-1) and presenilin enhancer 2 (PEN-2).
- Pathways
Presenilin 2 is central to the amyloidogenic pathway through its role in APP processing. This pathway links to Alzheimer's disease where dysregulation of amyloid beta peptide production leads to plaque formation. Additionally presenilin 2 takes part in the NOTCH signaling pathway which influences neurogenesis and cellular differentiation. Presenilin 1 is a partner protein of presenilin 2 within these pathways sharing overlapping functions and regulation.
- Associated diseases and disorders
Presenilin 2's connection to Alzheimer's disease arises from its role in amyloid beta production. Mutations in the PSEN2 gene can lead to familial Alzheimer's disease where altered processing of APP results in neurotoxic peptide accumulation. Additionally presenilin 2 influences cardiovascular disease development through its involvement in cell signaling pathways affecting vascular biology. Presenilin 1 mutations also associate with similar disorders due to its analogous role and participation in these pathological processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Presenilin 2/AD5 (phospho S327) antibody [EP2613Y] - BSA and Azide free (ab284741), expandable thumbnail](https://content.abcam.com/products/images/presenilin-2-ad5-phospho-s327-antibody-ep2613y-bsa-and-azide-free-ab284741--western-blot-img422021.jpg "Western blot - Anti-Presenilin 2/AD5 (phospho S327) antibody [EP2613Y] - BSA and Azide free (ab284741)")
Western blot - Anti-Presenilin 2/AD5 (phospho S327) antibody [EP2613Y] - BSA and Azide free (ab284741)
This data was developed using Anti-Presenilin 2/AD5 (phospho S327) antibody [EP2613Y] ab76114, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST,
Primary antibody: incubated for 1h at room temperature.
Secondary antibody: incubated for 1h at 1:20,000 at room temperature (Goat Anti-Rabbit IgG H&L (HRP) ab97051); incubated for 1h at 1:2000 at room temperature (Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG)All lanes: Western blot - Anti-Presenilin 2/AD5 (phospho S327) antibody [EP2613Y] (Anti-Presenilin 2/AD5 (phospho S327) antibody [EP2613Y] ab76114) at 1/500 dilution
Lane 1: RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 15 µg
Lane 2: RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate. Then the membrane was incubated with alkaline phosphatase at 15 µg
![Dot Blot - Anti-Presenilin 2/AD5 (phospho S327) antibody [EP2613Y] - BSA and Azide free (ab284741), expandable thumbnail](https://content.abcam.com/products/images/presenilin-2-ad5-phospho-s327-antibody-ep2613y-bsa-and-azide-free-ab284741--dot-blot-img422019.jpg "Dot Blot - Anti-Presenilin 2/AD5 (phospho S327) antibody [EP2613Y] - BSA and Azide free (ab284741)")
Dot Blot - Anti-Presenilin 2/AD5 (phospho S327) antibody [EP2613Y] - BSA and Azide free (ab284741)
This data was developed using Anti-Presenilin 2/AD5 (phospho S327) antibody [EP2613Y] ab76114, the same antibody clone in a different buffer formulation.
Dot blot analysis labelling Presenilin 2/AD5 (phospho S327) with Anti-Presenilin 2/AD5 (phospho S327) antibody [EP2613Y] ab76114 at 1/500 dilution.
Lane 1: Presenilin 2/AD5 (phospho S327) peptide
Lane 2: Presenilin 2/AD5 non-phospho peptide
A peroxidase-conjugated goat anti-rabbit IgG (H+L) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) was used as the secondary antibody at a dilution of 1/100,000.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 180s.![Western blot - Anti-Presenilin 2/AD5 (phospho S327) antibody [EP2613Y] - BSA and Azide free (ab284741), expandable thumbnail](https://content.abcam.com/products/images/presenilin-2-ad5-phospho-s327-antibody-ep2613y-bsa-and-azide-free-ab284741--western-blot-img422020.jpg "Western blot - Anti-Presenilin 2/AD5 (phospho S327) antibody [EP2613Y] - BSA and Azide free (ab284741)")
Western blot - Anti-Presenilin 2/AD5 (phospho S327) antibody [EP2613Y] - BSA and Azide free (ab284741)
This data was developed using Anti-Presenilin 2/AD5 (phospho S327) antibody [EP2613Y] ab76114, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST,
Primary antibody: incubated for 1h at room temperature.
Secondary antibody: incubated for 1h at 1:20,000 at room temperature (Goat Anti-Rabbit IgG H&L (HRP) ab97051); incubated for 1h at 1:2000 at room temperature (Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG)All lanes: Western blot - Anti-Presenilin 2/AD5 (phospho S327) antibody [EP2613Y] (Anti-Presenilin 2/AD5 (phospho S327) antibody [EP2613Y] ab76114) at 1/500 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate. Then the membrane was incubated with alkaline phosphatase at 15 µg
Exposure time: 180s
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