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AB284784

Anti-Presenilin 2/AD5 (phospho S330) antibody [EP2614] - BSA and Azide free

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Rabbit Monoclonal Presenilin 2/AD5 phospho S330 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

AD4, PS2, PSNL2, STM2, PSEN2, Presenilin-2, PS-2, AD3LP, AD5, E5-1, STM-2

1 Images
Western blot - Anti-Presenilin 2/AD5 (phospho S330) antibody [EP2614] - BSA and Azide free (AB284784)
  • WB

Unknown

Western blot - Anti-Presenilin 2/AD5 (phospho S330) antibody [EP2614] - BSA and Azide free (AB284784)

This data was developed using ab109456, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Presenilin 2/AD5 (phospho S330) antibody [EP2614] (<a href='/en-us/products/primary-antibodies/presenilin-2-ad5-phospho-s330-antibody-ep2614-ab109456'>ab109456</a>) at 1/5000 dilution

Lane 1:

HeLa cell lysate untreated with with Alkaline Phosphatase at 10 µg

Lane 2:

HeLa cell lysate treated with Alkaline Phosphatase at 10 µg

Secondary

All lanes:

HRP-labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 50 kDa

false

  • Unconjugated

    Anti-Presenilin 2/AD5 (phospho S330) antibody [EP2614]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2614

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab284784 is the carrier-free version of ab109456

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The presenilin 2 (PSEN2) protein also known as AD5 plays a role as a catalytic subunit in the gamma-secretase complex. This complex is involved in intramembrane proteolysis of several type-I transmembrane proteins. PSEN2 has a mass of about 52 kDa and is expressed mainly in the brain and several other tissues throughout the body. Its gene is located on chromosome 1 and has the alternative name 'EP-2614'. Presenilin 2 is closely associated with presenilin 1 (PSEN1) a related protein within the same family.
Biological function summary

Presenilin 2 functions in the regulation of proteolytic processing of the amyloid precursor protein (APP) within the gamma-secretase complex. This action produces amyloid beta peptides which are critical for normal brain function but may accumulate under pathological conditions. The protein is also involved in NOTCH receptor activation which plays a part in cell fate determination during development. The gamma-secretase complex includes other key proteins such as nicastrin anterior pharynx-defective 1 (APH-1) and presenilin enhancer 2 (PEN-2).

Pathways

Presenilin 2 is central to the amyloidogenic pathway through its role in APP processing. This pathway links to Alzheimer's disease where dysregulation of amyloid beta peptide production leads to plaque formation. Additionally presenilin 2 takes part in the NOTCH signaling pathway which influences neurogenesis and cellular differentiation. Presenilin 1 is a partner protein of presenilin 2 within these pathways sharing overlapping functions and regulation.

Presenilin 2's connection to Alzheimer's disease arises from its role in amyloid beta production. Mutations in the PSEN2 gene can lead to familial Alzheimer's disease where altered processing of APP results in neurotoxic peptide accumulation. Additionally presenilin 2 influences cardiovascular disease development through its involvement in cell signaling pathways affecting vascular biology. Presenilin 1 mutations also associate with similar disorders due to its analogous role and participation in these pathological processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein) (PubMed : 10497236, PubMed : 10652302, PubMed : 16752394, PubMed : 27293189, PubMed : 36272978). Selectively cleaves late endosomal/lysosomal localized substrates and generates the prominent pool of intracellular amyloid beta that contains longer amyloid beta (PubMed : 27293189). The holoprotein functions as a calcium-leak channel that allows the passive movement of calcium from endoplasmic reticulum to cytosol and is involved in calcium homeostasis (PubMed : 16959576). Is a regulator of mitochondrion-endoplasmic reticulum membrane tethering and modulates calcium ions shuttling between ER and mitochondria (PubMed : 21285369).
See full target information PSEN2 pS330
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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