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AB223698

Anti-PREX1 antibody

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(1 Publication)

Rabbit Polyclonal PREX1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PREX1 aa 700-900.

View Alternative Names

KIAA1415, PREX1, P-Rex1

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PREX1 antibody (AB223698)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PREX1 antibody (AB223698)

Paraffin-embedded human lymph node tissue stained for PREX1 using ab223698 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-PREX1 antibody (AB223698)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PREX1 antibody (AB223698)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for PREX1 (green) using ab223698 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PREX1 antibody (AB223698)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PREX1 antibody (AB223698)

Paraffin-embedded human small intestine tissue stained for PREX1 using ab223698 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PREX1 antibody (AB223698)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PREX1 antibody (AB223698)

Paraffin-embedded human hippocampus tissue stained for PREX1 using ab223698 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PREX1 antibody (AB223698)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PREX1 antibody (AB223698)

Paraffin-embedded human kidney tissue stained for PREX1 using ab223698 at 1/200 dilution in immunohistochemical analysis. Negative control - no staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human PREX1 aa 700-900. The exact immunogen used to generate this antibody is proprietary information.

Q8TCU6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PREX1 protein also known as phosphatidylinositol 345-trisphosphate-dependent Rac exchange factor 1 operates as a guanine nucleotide exchange factor (GEF). It facilitates the activation of the small GTPase RAC1 by exchanging GDP for GTP. The protein has a molecular mass of approximately 185 kDa. PREX1 is widely expressed in various tissues with higher levels observed in brain heart and leukocytes. Additionally it contains several distinct domains including the DH domain responsible for its GEF activity PH domain for lipid binding and DEP domain aiding in signaling pathway integration.
Biological function summary

PREX1 plays a fundamental role in modulating cellular processes such as cell migration growth and survival. It interacts with RAC1 enabling the restructuring of the actin cytoskeleton and promoting cell motility. PREX1 does not only function in isolation but is often found within complexes that influence RAC1 activity. It cooperates with proteins like PI3K (phosphoinositide 3-kinase) enhancing downstream signaling events integral to cellular function.

Pathways

PREX1 participates in pathways like PI3K/Akt and RAC1 signaling. These pathways are pivotal in regulating cellular responses to external stimuli. Interaction with PI3K leads to PREX1 activation by PIP3 which in turn activates RAC1. This association is important for signal transduction involved in cellular growth and survival. Furthermore PREX1's interaction with proteins like AKT and mTOR highlights its involvement in metabolic regulation and cellular homeostasis.

PREX1 exhibits significant correlation with cancer and cardiovascular diseases. Aberrant PREX1 expression or function often leads to altered RAC1 signaling contributing to tumorigenesis and metastasis in cancers such as breast cancer. The dysregulation of PREX1 also associates with cardiovascular conditions by influencing vascular tone and heart muscle contractility. These disease associations emphasize interactions with proteins like PI3K and RAC1 highlighting their roles in disease pathophysiology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions as a RAC guanine nucleotide exchange factor (GEF), which activates the Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol 3,4,5-trisphosphate and the beta gamma subunits of heterotrimeric G protein. May function downstream of heterotrimeric G proteins in neutrophils.
See full target information PREX1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Reproduction (Cambridge, England) 167: PubMed38236723

2024

Decreased NSD2 impairs stromal cell proliferation in human endometrium via reprogramming H3K36me2.

Applications

Unspecified application

Species

Unspecified reactive species

Chuan-Mei Qin,Xiao-Wei Wei,Jia-Yi Wu,Xue-Qing Liu,Yi Lin
View all publications
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Product promise

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