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AB121462

Anti-PREX2 antibody

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(4 Publications)

Rabbit Polyclonal PREX2 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PREX2 aa 650-850.

View Alternative Names

DEPDC2, PREX2, P-Rex2, DEP domain-containing protein 2

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PREX2 antibody (AB121462)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PREX2 antibody (AB121462)

ab121462 at a 1/725 dilution staining PREX2 in Human stomach tissue (paraffin embedded) by immunohistochemistry.

Immunocytochemistry/ Immunofluorescence - Anti-PREX2 antibody (AB121462)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PREX2 antibody (AB121462)

Immunofluorescent staining of Human cell line U-2 OS shows positivity in nuclei but not nucleoli. Recommended concentration of ab121462 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human PREX2 aa 650-850. The exact immunogen used to generate this antibody is proprietary information.

Q70Z35

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-4 µg/mL", "ICCIF-species-notes": "<p>Recommend PFA Fixation and Triton X-100 treatment</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PREX2 also known as PIP3-dependent Rac exchanger 2 acts as a guanine nucleotide exchange factor (GEF) specifically regulating the small GTPase Rac1. The PREX2 protein has a mass of approximately 212 kDa and is expressed abundantly in the brain but can also be found in other tissues. It facilitates the switch of Rac1 from its inactive GDP-bound state to the active GTP-bound state thereby modulating cell signaling processes related to actin cytoskeleton reorganization and cell migration.
Biological function summary

PREX2 significantly influences cell movement and growth. It operates as part of a multi-protein complex that coordinates various cellular signaling pathways. In cell migration and invasion PREX2 collaborates with other GEFs to promote Rac1 activation affecting processes like cell morphology and motility. Furthermore its role extends to insulin signaling where it interacts with components critical in glucose homeostasis.

Pathways

PREX2 engages in the phosphoinositide 3-kinase (PI3K) pathway a vital signaling cascade regulating growth metabolism and apoptosis. PI3K generates phosphatidylinositol (345)-trisphosphate (PIP3) which in turn facilitates PREX2 activation. Through the PI3K pathway PREX2 works alongside proteins like PTEN an important phosphatase that negatively regulates the PI3K pathway balancing cell survival and growth signals. PREX2 also interacts with the mTOR pathway impacting cell proliferation and energy balance.

PREX2 has been linked to cancer and autism spectrum disorders. Its involvement in cancer is noted through its regulation of Rac1 which affects tumor cell motility and invasiveness. Mutations and dysregulation in PREX2 may lead to enhanced tumor growth and metastasis. PREX2 also has potential interactions with proteins such as PTEN in oncogenic pathways. In autism spectrum disorders variation in PREX2 may contribute to altered neuronal signaling affecting brain development and function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions as a RAC1 guanine nucleotide exchange factor (GEF), activating Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol 3,4,5-trisphosphate and the beta gamma subunits of heterotrimeric G protein. Mediates the activation of RAC1 in a PI3K-dependent manner. May be an important mediator of Rac signaling, acting directly downstream of both G protein-coupled receptors and phosphoinositide 3-kinase.
See full target information PREX2
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Carcinogenesis 41:377-389 PubMed31241130

2019

CELF2 suppresses non-small cell lung carcinoma growth by inhibiting the PREX2-PTEN interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Yiu To Yeung,Suyu Fan,Bingbing Lu,Shuying Yin,Sen Yang,Wenna Nie,Meixian Wang,Liting Zhou,Tiepeng Li,Xiang Li,Ann M Bode,Zigang Dong

Oncotarget 9:24778-24786 PubMed29872505

2018

Expression of P-REX2a is associated with poor prognosis in endometrial malignancies.

Applications

Unspecified application

Species

Unspecified reactive species

Sho Takeshita,Yoriko Yamashita,Kosuke Shiomi,Nako Suzuki,Jun Yoshida,Aya Naiki-Ito,Shugo Suzuki,Shinya Akatsuka,Shinya Toyokuni,Takashi Takahashi,Shoko Mase,Atsushi Arakawa,Mayumi Sugiura-Ogasawara,Satoru Takahashi

Oncology letters 11:2223-2228 PubMed26998152

2016

Upregulation of PREX2 promotes the proliferation and migration of hepatocellular carcinoma cells via PTEN-AKT signaling.

Applications

Unspecified application

Species

Human

Shujie He,Juan Lin,Shaoping Yu,Shijie Sun

Molecular medicine reports 13:2301-7 PubMed26795161

2016

Knockdown of PREX2a inhibits the malignant phenotype of glioma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Liu,Zhixiong Liu,Hao Wang,Long Chen,Fuqiang Ruan,Jihui Zhang,Yi Hu,Hengshan Luo,Shuai Wen
View all publications
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Product promise

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