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AB169027

Anti-PREX2 antibody

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(6 Publications)

Mouse Polyclonal PREX2 antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PREX2.

View Alternative Names

DEPDC2, PREX2, P-Rex2, DEP domain-containing protein 2

1 Images
Western blot - Anti-PREX2 antibody (AB169027)
  • WB

Unknown

Western blot - Anti-PREX2 antibody (AB169027)

All lanes:

Western blot - Anti-PREX2 antibody (ab169027) at 1 µg/mL

Lane 1:

PREX2 transfected 293T cell line lysate at 15 µL

Lane 2:

Non-transfected 293T cell line lysate at 15 µL

Predicted band size: 183 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human PREX2.

Q70Z35

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PREX2 also known as PIP3-dependent Rac exchanger 2 acts as a guanine nucleotide exchange factor (GEF) specifically regulating the small GTPase Rac1. The PREX2 protein has a mass of approximately 212 kDa and is expressed abundantly in the brain but can also be found in other tissues. It facilitates the switch of Rac1 from its inactive GDP-bound state to the active GTP-bound state thereby modulating cell signaling processes related to actin cytoskeleton reorganization and cell migration.
Biological function summary

PREX2 significantly influences cell movement and growth. It operates as part of a multi-protein complex that coordinates various cellular signaling pathways. In cell migration and invasion PREX2 collaborates with other GEFs to promote Rac1 activation affecting processes like cell morphology and motility. Furthermore its role extends to insulin signaling where it interacts with components critical in glucose homeostasis.

Pathways

PREX2 engages in the phosphoinositide 3-kinase (PI3K) pathway a vital signaling cascade regulating growth metabolism and apoptosis. PI3K generates phosphatidylinositol (345)-trisphosphate (PIP3) which in turn facilitates PREX2 activation. Through the PI3K pathway PREX2 works alongside proteins like PTEN an important phosphatase that negatively regulates the PI3K pathway balancing cell survival and growth signals. PREX2 also interacts with the mTOR pathway impacting cell proliferation and energy balance.

PREX2 has been linked to cancer and autism spectrum disorders. Its involvement in cancer is noted through its regulation of Rac1 which affects tumor cell motility and invasiveness. Mutations and dysregulation in PREX2 may lead to enhanced tumor growth and metastasis. PREX2 also has potential interactions with proteins such as PTEN in oncogenic pathways. In autism spectrum disorders variation in PREX2 may contribute to altered neuronal signaling affecting brain development and function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions as a RAC1 guanine nucleotide exchange factor (GEF), activating Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol 3,4,5-trisphosphate and the beta gamma subunits of heterotrimeric G protein. Mediates the activation of RAC1 in a PI3K-dependent manner. May be an important mediator of Rac signaling, acting directly downstream of both G protein-coupled receptors and phosphoinositide 3-kinase.
See full target information PREX2
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Oncology reports 47: PubMed34958114

2021

Methylation and mutation of the inhibin‑α gene in human melanoma cells and regulation of PTEN expression and AKT/PI3K signaling by a demethylating agent.

Applications

Unspecified application

Species

Unspecified reactive species

Hyunmin Ko,Hyung Joon Ahn,Young Il Kim

Oncology letters 15:3185-3191 PubMed29435055

2018

Interference of P-REX2a may inhibit proliferation and reverse the resistance of SGC7901 cells to doxorubicin.

Applications

Unspecified application

Species

Unspecified reactive species

Yaowei Ai,Qiaohui Zhou,Ling Li,Zhihong Pan,Mingwen Guo,Jingbo Han

eLife 6: PubMed28100394

2017

Replication Study: Melanoma genome sequencing reveals frequent mutations.

Applications

Unspecified application

Species

Unspecified reactive species

Stephen K Horrigan,Pascal Courville,Darryl Sampey,Faren Zhou,Steve Cai

Oncology letters 12:1139-1143 PubMed27446408

2016

PREX2 promotes the proliferation, invasion and migration of pancreatic cancer cells by modulating the PI3K signaling pathway.

Applications

WB

Species

Human

Jianyi Yang,Xuejun Gong,Lu Ouyang,Wen He,Rou Xiao,Li Tan

Molecular medicine reports 13:1930-6 PubMed26718453

2016

Knockdown of PREX2a inhibits the malignant phenotype of osteosarcoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zixun Dai,Jie Xie,Pengfei Lei,Yihe Hu

eLife 3: PubMed25490935

2014

Registered report: Melanoma genome sequencing reveals frequent PREX2 mutations.

Applications

Unspecified application

Species

Unspecified reactive species

Denise Chroscinski,Darryl Sampey,Alex Hewitt
View all publications
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