Mouse Polyclonal PREX2 antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PREX2.
Images
Publications
Filter by
- Oncology reports 47:2021Methylation and mutation of the inhibin‑α gene in human melanoma cells and regulation of PTEN expression and AKT/PI3K signaling by a demethylating agent.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyunmin Ko,Hyung Joon Ahn,Young Il KimPubMed 34958114
- Oncology letters 15:3185-31912018Interference of P-REX2a may inhibit proliferation and reverse the resistance of SGC7901 cells to doxorubicin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYaowei Ai,Qiaohui Zhou,Ling Li,Zhihong Pan,Mingwen Guo,Jingbo HanPubMed 29435055
- eLife 6:2017Replication Study: Melanoma genome sequencing reveals frequent mutations.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStephen K Horrigan et. al.PubMed 28100394
- Oncology letters 12:1139-11432016PREX2 promotes the proliferation, invasion and migration of pancreatic cancer cells by modulating the PI3K signaling pathway.
- Molecular medicine reports 13:1930-62016Knockdown of PREX2a inhibits the malignant phenotype of osteosarcoma cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZixun Dai,Jie Xie,Pengfei Lei,Yihe HuPubMed 26718453
- eLife 3:2014Registered report: Melanoma genome sequencing reveals frequent PREX2 mutations.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDenise Chroscinski et. al.PubMed 25490935
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human PREX2. Database link Q70Z35
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Functions as a RAC1 guanine nucleotide exchange factor (GEF), activating Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol 3,4,5-trisphosphate and the beta gamma subunits of heterotrimeric G protein. Mediates the activation of RAC1 in a PI3K-dependent manner. May be an important mediator of Rac signaling, acting directly downstream of both G protein-coupled receptors and phosphoinositide 3-kinase.
Alternative names
DEPDC2, PREX2, P-Rex2, DEP domain-containing protein 2
Recommended products
Mouse Polyclonal PREX2 antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PREX2.
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human PREX2. Database link Q70Z35
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PREX2 also known as PIP3-dependent Rac exchanger 2 acts as a guanine nucleotide exchange factor (GEF) specifically regulating the small GTPase Rac1. The PREX2 protein has a mass of approximately 212 kDa and is expressed abundantly in the brain but can also be found in other tissues. It facilitates the switch of Rac1 from its inactive GDP-bound state to the active GTP-bound state thereby modulating cell signaling processes related to actin cytoskeleton reorganization and cell migration.
- Biological function summary
PREX2 significantly influences cell movement and growth. It operates as part of a multi-protein complex that coordinates various cellular signaling pathways. In cell migration and invasion PREX2 collaborates with other GEFs to promote Rac1 activation affecting processes like cell morphology and motility. Furthermore its role extends to insulin signaling where it interacts with components critical in glucose homeostasis.
- Pathways
PREX2 engages in the phosphoinositide 3-kinase (PI3K) pathway a vital signaling cascade regulating growth metabolism and apoptosis. PI3K generates phosphatidylinositol (345)-trisphosphate (PIP3) which in turn facilitates PREX2 activation. Through the PI3K pathway PREX2 works alongside proteins like PTEN an important phosphatase that negatively regulates the PI3K pathway balancing cell survival and growth signals. PREX2 also interacts with the mTOR pathway impacting cell proliferation and energy balance.
- Associated diseases and disorders
PREX2 has been linked to cancer and autism spectrum disorders. Its involvement in cancer is noted through its regulation of Rac1 which affects tumor cell motility and invasiveness. Mutations and dysregulation in PREX2 may lead to enhanced tumor growth and metastasis. PREX2 also has potential interactions with proteins such as PTEN in oncogenic pathways. In autism spectrum disorders variation in PREX2 may contribute to altered neuronal signaling affecting brain development and function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Western blot - Anti-PREX2 antibody (ab169027)
All lanes: Western blot - Anti-PREX2 antibody (ab169027) at 1 µg/mL
Lane 1: PREX2 transfected 293T cell line lysate at 15 µL
Lane 2: Non-transfected 293T cell line lysate at 15 µL
Predicted band size: 183 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com