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AB187523

Anti-PRG2 antibody

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(5 Publications)

Rabbit Polyclonal PRG2 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PRG2.

View Alternative Names

MBP, PRG2, Bone marrow proteoglycan, BMPG, Proteoglycan 2

1 Images
Western blot - Anti-PRG2 antibody (AB187523)
  • WB

Supplier Data

Western blot - Anti-PRG2 antibody (AB187523)

Blocking/Diluting buffer : 3% NFDM/TBST

All lanes:

Western blot - Anti-PRG2 antibody (ab187523) at 1/1000 dilution

Lane 1:

L-02 (human adenocarcinoma cell line) cell lysate at 25 µg

Lane 2:

Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 25 µg

Lane 3:

K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate at 25 µg

Lane 4:

THP-1 (human monocytic leukemia cell line) cell lysate at 25 µg

Lane 5:

HL-60 (human promyelocytic leukemia cell line) cell lysate at 25 µg

Lane 6:

Mouse liver tissue lysate at 25 µg

Lane 7:

Mouse spleen tissue lysate at 25 µg

Secondary

All lanes:

HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 25 kDa

Observed band size: 23 kDa

true

Exposure time: 1s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human PRG2.

P13727

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PRG2 also known as major basic protein (MBP) is a potent protein that plays a significant role in the immune system. It weighs approximately 14 kDa and is found mainly in eosinophil granules. Eosinophils release PRG2 during the immune response which can affect various bodily systems. The expression of PRG2 is not limited to eosinophils; it is also found in other cell types that participate in immune responses.
Biological function summary

PRG2 interacts with other components of the immune system to help mediate inflammatory responses. It is a part of the innate immune response and acts by binding to and neutralizing negatively charged surfaces on cells pathogens and tissues. PRG2 can cause tissue damage by its cytotoxic action when released in large amounts impacting cells within its local environment. This protein does not belong to any known larger protein complex but its activity can influence many immune pathways independently.

Pathways

PRG2 contributes significantly to the immune system's function and the body's defense mechanism against parasites and allergens. The protein plays an important role in the allergic inflammatory pathway where it can interact with interleukins such as IL-5 which influences eosinophil activation and survival. Additionally PRG2 impacts the Th2 cytokine pathway coordinating with proteins like IL-4 to modulate immune responses.

High levels of PRG2 associate with conditions like asthma and allergic rhinitis. Its interaction with allergens and subsequent tissue damage leads to inflammation and disease exacerbation. Moreover elevated PRG2 levels are seen in Helminth infections where it coordinates with eosinophil cationic protein to combat parasites. These interactions make PRG2 a potential target for therapeutic intervention in related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytotoxin and helminthotoxin. Also induces non-cytolytic histamine release from human basophils. Involved in antiparasitic defense mechanisms and immune hypersensitivity reactions. The proform acts as a proteinase inhibitor, reducing the activity of PAPPA.
See full target information PRG2
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cell host & microbe 29:579-593.e5 PubMed33857419

2021

Il4ra-independent vaginal eosinophil accumulation following helminth infection exacerbates epithelial ulcerative pathology of HSV-2 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Alisha Chetty,Matthew G Darby,Pia M Vornewald,Mara Martín-Alonso,Anna Filz,Manuel Ritter,Henry J McSorley,Lindi Masson,Katherine Smith,Frank Brombacher,Matthew K O'Shea,Adam F Cunningham,Bernhard Ryffel,Menno J Oudhoff,Benjamin G Dewals,Laura E Layland,William G C Horsnell

Biochemical and biophysical research communication 513:635-641 PubMed30981508

2019

Construction of an irreversible allergic rhinitis-induced olfactory loss mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Caiquan Liang,Zixuan Yang,Qingyun Zou,Mengxia Zhou,Huanhai Liu,Jingping Fan

Medical science monitor : international medical jo 24:2377-2383 PubMed29672471

2018

Activated Eosinophils are Present in Esophageal Muscle in Patients with Achalasia of the Esophagus.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Jin,Bin Wang,Li-Li Zhang,Wei Zhao

Acta dermato-venereologica 97:464-471 PubMed27868136

2016

Increased Activity and Apoptosis of Eosinophils in Blister Fluids, Skin and Peripheral Blood of Patients with Bullous Pemphigoid.

Applications

Unspecified application

Species

Unspecified reactive species

Judith Engmann,Urda Rüdrich,Georg Behrens,Eleni Papakonstantinou,Manuela Gehring,Alexander Kapp,Ulrike Raap

Scientific reports 6:35559 PubMed27752109

2016

Wolbachia endosymbionts induce neutrophil extracellular trap formation in human onchocerciasis.

Applications

IHC-P

Species

Human

Francesca Tamarozzi,Joseph D Turner,Nicolas Pionnier,Angela Midgley,Ana F Guimaraes,Kelly L Johnston,Steven W Edwards,Mark J Taylor
View all publications
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