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AB322142

Anti-PRG2 antibody [EPR29096-502]

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Rabbit Recombinant Monoclonal PRG2 antibody. Suitable for IHC-P, I-ELISA and reacts with Human, Recombinant fragment - Human samples.

View Alternative Names

MBP, PRG2, Bone marrow proteoglycan, BMPG, Proteoglycan 2

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRG2 antibody [EPR29096-502] (AB322142)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRG2 antibody [EPR29096-502] (AB322142)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling PRG2 with ab322142 at 1/5000 (0.095 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining in human cerebrum. The section was incubated with ab322142 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRG2 antibody [EPR29096-502] (AB322142)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRG2 antibody [EPR29096-502] (AB322142)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PRG2 with ab322142 at 1/5000 (0.095 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining in human colon (PMID : 10491647). The section was incubated with ab322142 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRG2 antibody [EPR29096-502] (AB322142)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRG2 antibody [EPR29096-502] (AB322142)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling PRG2 with ab322142 at 1/5000 (0.095 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining in human placenta. The section was incubated with ab322142 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRG2 antibody [EPR29096-502] (AB322142)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRG2 antibody [EPR29096-502] (AB322142)

Immunohistochemical analysis of paraffin-embedded Human bone marrow tissue labeling PRG2 with ab322142 at 1/5000 (0.095 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining in human bone marrow. The section was incubated with ab322142 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Multiplex immunohistochemistry - Anti-PRG2 antibody [EPR29096-502] (AB322142)
  • mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-PRG2 antibody [EPR29096-502] (AB322142)

Immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded sections human bone marrow tissue labelling PRG2 with ab322142 at 5000 dilution (B), CD1777 with ab255296 at 2000 dilution (C) and CD42d/GP5 with ab322703 at 500 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with TrisEDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : anti-PRG2 (green; Opal™520), anti-CD177 (magenta; Opal™690) and anti-CD42d/GP5 (gray; Opal™570) on human bond marrow.

Panel B : anti-PRG2 staining basophils in human bone marrow.

Panel C : anti-CD177 staining neutrophils in human bone marrow.

Panel D : anti-CD42d/PGP5 staining megakaryocytes in human bone marrow.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab322142, ab255296 and ab322703 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Indirect ELISA - Anti-PRG2 antibody [EPR29096-502] (AB322142)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-PRG2 antibody [EPR29096-502] (AB322142)

Indirect ELISA analysis of ab322142 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution.

Antigen : Human PRG2,Human PRG3.

Antigen concentration : 1000 ng/ml

This antibody does not cross-react with human PRG3.

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-PRG2 antibody [EPR29096-502]

  • HRP

    HRP Anti-PRG2 antibody [EPR29096-502]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-PRG2 antibody [EPR29096-502]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29096-502

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

I-ELISA, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "62.5 ng/mL", "IELISA-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PRG2 also known as major basic protein (MBP) is a potent protein that plays a significant role in the immune system. It weighs approximately 14 kDa and is found mainly in eosinophil granules. Eosinophils release PRG2 during the immune response which can affect various bodily systems. The expression of PRG2 is not limited to eosinophils; it is also found in other cell types that participate in immune responses.
Biological function summary

PRG2 interacts with other components of the immune system to help mediate inflammatory responses. It is a part of the innate immune response and acts by binding to and neutralizing negatively charged surfaces on cells pathogens and tissues. PRG2 can cause tissue damage by its cytotoxic action when released in large amounts impacting cells within its local environment. This protein does not belong to any known larger protein complex but its activity can influence many immune pathways independently.

Pathways

PRG2 contributes significantly to the immune system's function and the body's defense mechanism against parasites and allergens. The protein plays an important role in the allergic inflammatory pathway where it can interact with interleukins such as IL-5 which influences eosinophil activation and survival. Additionally PRG2 impacts the Th2 cytokine pathway coordinating with proteins like IL-4 to modulate immune responses.

High levels of PRG2 associate with conditions like asthma and allergic rhinitis. Its interaction with allergens and subsequent tissue damage leads to inflammation and disease exacerbation. Moreover elevated PRG2 levels are seen in Helminth infections where it coordinates with eosinophil cationic protein to combat parasites. These interactions make PRG2 a potential target for therapeutic intervention in related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytotoxin and helminthotoxin. Also induces non-cytolytic histamine release from human basophils. Involved in antiparasitic defense mechanisms and immune hypersensitivity reactions. The proform acts as a proteinase inhibitor, reducing the activity of PAPPA.
See full target information PRG2
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