JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB182142

Anti-PRG3 antibody [EPR13990]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal PRG3 antibody. Suitable for WB and reacts with Human samples.

View Alternative Names

MBPH, UNQ486/PRO1002, PRG3, Proteoglycan 3, Eosinophil major basic protein homolog, Prepro-major basic protein homolog, Prepro-MBPH

1 Images
Western blot - Anti-PRG3 antibody [EPR13990] (AB182142)
  • WB

Supplier Data

Western blot - Anti-PRG3 antibody [EPR13990] (AB182142)

All lanes:

Western blot - Anti-PRG3 antibody [EPR13990] (ab182142) at 1/2000 dilution

All lanes:

Human bone marrow lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 25 kDa

Observed band size: 25 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13990

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PRG3 also known as Proteoglycan 3 or pro-eosinophil major basic protein 2 (ProMBP2) is a protein involved in various mechanical functions in the body. It carries a mass of approximately 22 kDa. The protein shows expression primarily in eosinophils a type of white blood cell and also appears in tissues where eosinophils are present like the lungs and gastrointestinal tract. The PRG3 protein localizes to eosinophil granules which are important for storing and releasing the granule contents that contribute to inflammation and immune response.
Biological function summary

PRG3 plays a role in the human immune system as it is involved in the body's response to parasitic infections and allergic reactions. PRG3 releases cytotoxic proteins that damage the membranes of pathogens and contribute to inflammation. This activity implicates PRG3 in the regulation of immune responses. PRG3 functions in collaboration with other proteins within the eosinophil working together with proteins like eosinophil peroxidase to enhance its immune activity.

Pathways

PRG3's role is significant within immune-related pathways such as the immune response pathway and the allergic pathway. In the immune response pathway PRG3 interacts with other immune system proteins like IL-5 which regulates eosinophil production and activation. Furthermore PRG3 contributes to the allergic pathway where it acts in unison with proteins like histamine amplifying allergic inflammation.

PRG3 has links to asthma and eosinophilic esophagitis. In asthma PRG3 contributes to inflammation and airway hyperreactivity by interacting with cytokines such as IL-5 and IL-13 which exacerbate the condition. In eosinophilic esophagitis PRG3's presence in eosinophil-rich infiltrates aggravates inflammation causing symptoms related to this disorder. Understanding these links provides insights into potential therapeutic strategies targeting PRG3 in related conditions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Possesses similar cytotoxic and cytostimulatory activities to PRG2/MBP. In vitro, stimulates neutrophil superoxide production and IL8 release, and histamine and leukotriene C4 release from basophils.
See full target information PRG3
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB108323

Anti-Perilipin 2 antibody [EPR3713]

RabMAb|Recombinant

Western blot - Anti-Perilipin 2 antibody [EPR3713] (AB108323)

  • 4
  • 3
Primary Antibodies

AB288715

Anti-S100A8 + S100A9 antibody [RM1038]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-S100A8 + S100A9 antibody [RM1038] (AB288715)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB192890

Anti-LC3B antibody [EPR18709] - Autophagosome Marker

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Western blot - Anti-LC3B antibody [EPR18709] - Autophagosome Marker (AB192890)

  • 5
  • 19

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com