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AB211419

Anti-Prickle 2 antibody

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(2 Publications)

Rabbit Polyclonal Prickle 2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human Prickle-like protein 2.

View Alternative Names

Prickle-like protein 2, PRICKLE2

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prickle 2 antibody (AB211419)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prickle 2 antibody (AB211419)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testis tissue, labeling Prickle 2 using ab211419 at 5 μg/ml.

Immunocytochemistry/ Immunofluorescence - Anti-Prickle 2 antibody (AB211419)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Prickle 2 antibody (AB211419)

Immunofluorescence analysis of rat brain cells, labeling Prickle 2 using ab211419 at a 20 μg/mL.

Western blot - Anti-Prickle 2 antibody (AB211419)
  • WB

Supplier Data

Western blot - Anti-Prickle 2 antibody (AB211419)

All lanes:

Western blot - Anti-Prickle 2 antibody (ab211419) at 1 µg/mL

All lanes:

mouse A20 cell lysate

Predicted band size: 96 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human Prickle-like protein 2. The exact immunogen used to generate this antibody is proprietary information.

Q7Z3G6

Specificity

ab211419 is predicted not to cross-react with other Prickle protein family memebers.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p>Perform heat-mediated antigen retrieval.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "20 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prickle 2 also known as PRICKLE2 or prickle-like protein 2 is a member of the prickle family of proteins. This protein weighs around 92 kDa and plays an important role in various cellular processes. It is involved in the planar cell polarity (PCP) signaling pathway and regulates directional signaling in tissues. Prickle 2 shows expression in the central nervous system and other tissues demonstrating a significant role in neural development and maintenance.
Biological function summary

Prickle 2 engages in the coordination of cell orientation and motility. It is part of the PCP complex which controls asymmetrical cell division and positioning. By participating in this complex Prickle 2 ensures cells align properly within the tissue matrix. It contributes to synaptic formation and function highlighting its involvement in neural connectivity and signaling within the brain.

Pathways

Prickle 2 takes part in the Wnt/PCP signaling pathway and interacts with key proteins such as Dishevelled and Frizzled. This pathway is essential for proper cell orientation and cytoskeletal rearrangement. Prickle 2 and its associated proteins coordinate to influence neural development strategies and maintain synaptic stability important for neural pathfinding and network establishment.

Prickle 2's dysfunction links to neural pathologies like epilepsy and autism spectrum disorders. Alterations in Prickle 2 expression can disrupt the Wnt/PCP pathway causing improper neuron positioning. Prickle 2 connects with proteins like Dishevelled in these contexts affecting the signaling cascades necessary for normal cognitive and developmental processes. Understanding these connections helps elucidate its role in maintaining neurological health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Prickle-like protein 2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Science advances 9:eadh2023 PubMed37672588

2023

Loss of attachment promotes proline accumulation and excretion in cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Steven E Pilley,Marc Hennequart,Anke Vandekeere,Julianna Blagih,Nathalie M Legrave,Sarah-Maria Fendt,Karen H Vousden,Christiaan F Labuschagne

Annals of translational medicine 9:498 PubMed33850895

2021

Circular RNA hsa-circ-000881 suppresses the progression of lung adenocarcinoma via a miR-665/PRICKLE2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Chu Huang,Weiming Yue,Lin Li,Shuhai Li,Cun Gao,Libo Si,Lei Qi,Chuanle Cheng,Ming Lu,Guanqing Chen,Jingjing Cui,Renchang Zhao,Yongmeng Li,Hui Tian
View all publications
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