Goat Polyclonal PRICKLE3 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human PRICKLE3 aa 500-550.
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Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human PRICKLE3 aa 500-550. The exact immunogen used to generate this antibody is proprietary information. Database link O43900
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.10000-0.30000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
Target data
Function
Involved in the planar cell polarity (PCP) pathway that is essential for the polarization of epithelial cells during morphogenetic processes, including gastrulation and neurulation (By similarity). PCP is maintained by two molecular modules, the global and the core modules, PRICKLE3 being part of the core module (By similarity). Distinct complexes of the core module segregate to opposite sides of the cell, where they interact with the opposite complex in the neighboring cell at or near the adherents junctions (By similarity). Involved in the organization of the basal body (By similarity). Involved in cilia growth and positioning (By similarity). Required for proper assembly, stability, and function of mitochondrial membrane ATP synthase (mitochondrial complex V) (PubMed:32516135).
Alternative names
LMO6, PRICKLE3, Prickle planar cell polarity protein 3, LIM domain only protein 6, Prickle-like protein 3, Triple LIM domain protein 6, LMO-6, Pk3
Recommended products
Goat Polyclonal PRICKLE3 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human PRICKLE3 aa 500-550.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human PRICKLE3 aa 500-550. The exact immunogen used to generate this antibody is proprietary information. Database link O43900
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab183522 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PRICKLE3 also known by other names such as Prickle-like protein 3 and Testis expressed protein 44 (TEX44) is a protein characterized by its mechanistic roles related to cellular signaling. It is a part of the Prickle family which is known for planar cell polarity functions. PRICKLE3 weighs approximately 60 kDa. Expression of PRICKLE3 is noted in various tissues with higher expression in brain and testis where it likely participates in critical cellular activities.
- Biological function summary
PRICKLE3 serves critical functions in cellular orientation and signaling pathways that are important for development and differentiation. It can form part of a protein complex implicated in intracellular signal transduction. This complex has been shown to interact with other proteins in the Prickle family to mediate planar cell polarity which is vital for various developmental processes both cellular and organismal.
- Pathways
PRICKLE3 engages in the Wnt signaling pathway and the planar cell polarity (PCP) signaling pathway. These pathways are important for controlling cell movement and positioning. In the PCP pathway PRICKLE3 works alongside other proteins such as Dishevelled Van Gogh and Flamingo to regulate cell alignment and directional growth. Disruptions in these pathways can have significant impacts on biological processes leading to developmental anomalies.
- Associated diseases and disorders
PRICKLE3 has been linked to conditions such as epilepsy and cognitive impairments. Alterations in its function or expression can influence synaptic activity and neurodevelopmental processes. In particular the protein's interaction with Dishevelled in the PCP pathway highlights its potential role in disorders where these pathways are dysfunctional. Understanding these connections can provide insight into new therapeutic avenues for these neurological conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-PRICKLE3 antibody (ab183522)
All lanes: Western blot - Anti-PRICKLE3 antibody (ab183522) at 0.1 µg/mL
All lanes: Daudi cell lysates in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 69 kDa
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