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AB183522

Anti-PRICKLE3 antibody

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(1 Publication)

Goat Polyclonal PRICKLE3 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PRICKLE3 aa 500-550.

View Alternative Names

LMO6, PRICKLE3, Prickle planar cell polarity protein 3, LIM domain only protein 6, Prickle-like protein 3, Triple LIM domain protein 6, LMO-6, Pk3

1 Images
Western blot - Anti-PRICKLE3 antibody (AB183522)
  • WB

Supplier Data

Western blot - Anti-PRICKLE3 antibody (AB183522)

All lanes:

Western blot - Anti-PRICKLE3 antibody (ab183522) at 0.1 µg/mL

All lanes:

Daudi cell lysates in RIPA buffer at 35 µg

Predicted band size: 69 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human PRICKLE3 aa 500-550. The exact immunogen used to generate this antibody is proprietary information.

O43900

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab183522 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PRICKLE3 also known by other names such as Prickle-like protein 3 and Testis expressed protein 44 (TEX44) is a protein characterized by its mechanistic roles related to cellular signaling. It is a part of the Prickle family which is known for planar cell polarity functions. PRICKLE3 weighs approximately 60 kDa. Expression of PRICKLE3 is noted in various tissues with higher expression in brain and testis where it likely participates in critical cellular activities.
Biological function summary

PRICKLE3 serves critical functions in cellular orientation and signaling pathways that are important for development and differentiation. It can form part of a protein complex implicated in intracellular signal transduction. This complex has been shown to interact with other proteins in the Prickle family to mediate planar cell polarity which is vital for various developmental processes both cellular and organismal.

Pathways

PRICKLE3 engages in the Wnt signaling pathway and the planar cell polarity (PCP) signaling pathway. These pathways are important for controlling cell movement and positioning. In the PCP pathway PRICKLE3 works alongside other proteins such as Dishevelled Van Gogh and Flamingo to regulate cell alignment and directional growth. Disruptions in these pathways can have significant impacts on biological processes leading to developmental anomalies.

PRICKLE3 has been linked to conditions such as epilepsy and cognitive impairments. Alterations in its function or expression can influence synaptic activity and neurodevelopmental processes. In particular the protein's interaction with Dishevelled in the PCP pathway highlights its potential role in disorders where these pathways are dysfunctional. Understanding these connections can provide insight into new therapeutic avenues for these neurological conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the planar cell polarity (PCP) pathway that is essential for the polarization of epithelial cells during morphogenetic processes, including gastrulation and neurulation (By similarity). PCP is maintained by two molecular modules, the global and the core modules, PRICKLE3 being part of the core module (By similarity). Distinct complexes of the core module segregate to opposite sides of the cell, where they interact with the opposite complex in the neighboring cell at or near the adherents junctions (By similarity). Involved in the organization of the basal body (By similarity). Involved in cilia growth and positioning (By similarity). Required for proper assembly, stability, and function of mitochondrial membrane ATP synthase (mitochondrial complex V) (PubMed : 32516135).
See full target information PRICKLE3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:7524 PubMed37980342

2023

The TDRD3-USP9X complex and MIB1 regulate TOP3B homeostasis and prevent deleterious TOP3B cleavage complexes.

Applications

Unspecified application

Species

Unspecified reactive species

Sourav Saha,Shar-Yin Naomi Huang,Xi Yang,Liton Kumar Saha,Yilun Sun,Prashant Khandagale,Lisa M Jenkins,Yves Pommier
View all publications
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