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Mouse Monoclonal Prion protein PrP antibody. Suitable for ICC, IP, Flow Cyt, WB, IHC-P, I-ELISA and reacts with Mouse, Rat, Monkey, Human, Cow, Sheep samples. Cited in 13 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Prnp.


Images

Key facts

Isotype

IgG2b

Host species

Mouse

Storage buffer

pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Mouse Prnp. Database link P04925
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCIPFlow CytWBIHC-PI-ELISA
Human
Expected
Expected
Expected
Predicted
Expected
Expected
Mouse
Expected
Expected
Expected
Tested
Expected
Expected
Rat
Expected
Expected
Expected
Predicted
Expected
Expected
Cow
Expected
Expected
Expected
Predicted
Expected
Expected
Monkey
Expected
Expected
Expected
Predicted
Expected
Expected
Sheep
Expected
Expected
Expected
Predicted
Expected
Expected

Expected
Expected

Species

Mouse, Rat, Monkey, Human, Cow, Sheep

Dilution info

Use at an assay dependent concentration.

Notes

-

Expected
Expected

Species

Mouse, Rat, Monkey, Human, Cow, Sheep

Dilution info

Use at an assay dependent concentration.

Notes

-

Expected
Expected

Species

Mouse

Dilution info

-

Notes

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Species

Rat

Dilution info

-

Notes

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Species

Monkey

Dilution info

-

Notes

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Species

Human

Dilution info

-

Notes

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Species

Cow

Dilution info

-

Notes

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Species

Sheep

Dilution info

-

Notes

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Predicted
Predicted

Species

Sheep, Monkey, Human, Cow, Rat

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat, Monkey, Human, Cow, Sheep

Dilution info

Use at an assay dependent concentration.

Notes

-

Expected
Expected

Species

Mouse, Rat, Monkey, Human, Cow, Sheep

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Its primary physiological function is unclear. May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May promote myelin homeostasis through acting as an agonist for ADGRG6 receptor. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro) (By similarity). Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts. Also provides Cu(2+) or ZN(2+) for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains (By similarity).

Alternative names

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Mouse Monoclonal Prion protein PrP antibody. Suitable for ICC, IP, Flow Cyt, WB, IHC-P, I-ELISA and reacts with Mouse, Rat, Monkey, Human, Cow, Sheep samples. Cited in 13 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Prnp.

Alternative names

Key facts

Isotype

IgG2b

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Full Length Protein corresponding to Mouse Prnp. Database link P04925
Clone number

8H4

Purity

IgG fraction

Epitope

The antibody epitope resides within amino acids 145-180 of human Prion protein PrP.

Concentration
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Purification notes

Purified Immunoglobulin

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

Prion protein assists in maintaining normal cell activities. Researchers do not fully understand its exact biological role but it might be involved in copper ion uptake and protection against oxidative stress. PrP can form complexes with other cellular proteins some of which help in routing signals inside the cell. Additionally prion protein may have synaptic functions related to neurodevelopment and neuroprotection.

Activity summary

Prion protein also known as PrP or major prion protein plays a mechanical role in the normal functioning of brain cells. It is a glycoprotein with a flexible structure and has an approximate mass of 35-36 kDa. PrP expression is high in nervous tissue. It is present in neurons and glial cells but also surfaces in other tissues like heart and kidney. Alternate names like p-pr-p and f89 refer to specific conformations or studies related to its structure.

Pathways

Prion protein links to both neuroprotective and neurodegenerative pathways. It participates in signaling pathways that protect neurons from apoptosis. This protein associates closely with copper-dependent pathways possibly related to its capacity to bind copper ions which affects oxidative stress responses. Prion protein also interacts with proteins like synapsin to modulate synaptic transmission.

Associated diseases and disorders

Prion protein is directly related to prion diseases such as Creutzfeldt-Jakob disease and kuru. These diseases arise from misfolded forms of PrP which aggregate and cause neurodegeneration. The misfolded form referred to as PrP^Sc can induce normal PrP to misfold propagating disease. Dopamine receptor proteins and synaptic proteins can indirectly interact or be affected in these disorders highlighting a complex network of affected neural functions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-Prion protein PrP antibody [8H4] (ab61409), expandable thumbnail

    Western blot - Anti-Prion protein PrP antibody [8H4] (ab61409)

    All lanes: Western blot - Anti-Prion protein PrP antibody [8H4] (AB61409) at 4 µg/mL

    All lanes: Mouse brain extract

    Secondary

    All lanes: Goat Anti-Mouse IgG-Peroxidase

    Predicted band size: 28 kDa

  • Western blot - Anti-Prion protein PrP antibody [8H4] (ab61409), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Western blot - Anti-Prion protein PrP antibody [8H4] (ab61409)

    All lanes: Western blot - Anti-Prion protein PrP antibody [8H4] (AB61409) at 1/2500 dilution

    Lane 1: Wild type mouse hippocampus whole tissue lysate at 30 µg

    Lane 2: Prnp-/- mouse hippocampus whole tissue lysate at 30 µg

    Secondary

    All lanes: HRP-conjugated sheep anti-mouse IgG monoclonal at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 28 kDa

    Observed band size: 25-37 kDa

    Exposure time: 30s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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