AB178545

Anti-Prion protein PrP antibody

Name: Anti-Prion protein PrP antibody (ab178545) | Abcam
Brand: Abcam Limited
SKU: AB178545
Price: 525 USD
Availability: InStock

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(2 Publications)

Chicken Polyclonal Prion protein PrP antibody. Suitable for ICC/IF and reacts with Recombinant fragment - Mouse samples. Cited in 2 publications.

View Alternative Names

CD230, ALTPRP, PRIP, PRP, PRNP, Major prion protein, PrP, ASCR, PrP27-30, PrP33-35C

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-Prion protein PrP antibody (AB178545)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Prion protein PrP antibody (AB178545)

Immunofluorescent analysis of mouse fibroblast cultures transfected with a recombinant fragment of the mouse prion protein (green staining). Non-infected cells show labeled nuclei (blue staining) due to treatment with Hoechst stain.

Key facts

Host species

Chicken

Clonality

Polyclonal

Isotype

IgY

Carrier free

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant fragment - Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein L

Storage buffer

pH: 7 Preservative: 0.02% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 0.16% Sodium phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prion protein also known as PrP or major prion protein plays a mechanical role in the normal functioning of brain cells. It is a glycoprotein with a flexible structure and has an approximate mass of 35-36 kDa. PrP expression is high in nervous tissue. It is present in neurons and glial cells but also surfaces in other tissues like heart and kidney. Alternate names like p-pr-p and f89 refer to specific conformations or studies related to its structure.

Biological function summary

Prion protein assists in maintaining normal cell activities. Researchers do not fully understand its exact biological role but it might be involved in copper ion uptake and protection against oxidative stress. PrP can form complexes with other cellular proteins some of which help in routing signals inside the cell. Additionally prion protein may have synaptic functions related to neurodevelopment and neuroprotection.

Pathways

Prion protein links to both neuroprotective and neurodegenerative pathways. It participates in signaling pathways that protect neurons from apoptosis. This protein associates closely with copper-dependent pathways possibly related to its capacity to bind copper ions which affects oxidative stress responses. Prion protein also interacts with proteins like synapsin to modulate synaptic transmission.

Prion protein is directly related to prion diseases such as Creutzfeldt-Jakob disease and kuru. These diseases arise from misfolded forms of PrP which aggregate and cause neurodegeneration. The misfolded form referred to as PrP^Sc can induce normal PrP to misfold propagating disease. Dopamine receptor proteins and synaptic proteins can indirectly interact or be affected in these disorders highlighting a complex network of affected neural functions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Its primary physiological function is unclear. May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May promote myelin homeostasis through acting as an agonist for ADGRG6 receptor. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro) (By similarity). Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts. Also provides Cu(2+) or Zn(2+) for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains (By similarity).

See full target information PRNP

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Prion 17:116-132 PubMed37131335

2023

Differentiated cultures of an immortalized human neural progenitor cell line do not replicate prions despite PrP overexpression.

Applications

Unspecified application

Species

Unspecified reactive species

Jessy A Slota,Xinzhu Wang,Diana Lusansky,Sarah J Medina,Stephanie A Booth

Journal of biological engineering 13:79 PubMed31673286

2019

A novel egg-shell membrane based hybrid nanofibrous scaffold for cutaneous tissue engineering.

Applications

Unspecified application

Species

Unspecified reactive species

Leila Mohammadzadeh,Reza Rahbarghazi,Roya Salehi,Mehrdad Mahkam

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Prion protein PrP antibody

Immunocytochemistry/ Immunofluorescence - Anti-Prion protein PrP antibody (AB178545)

Key facts

Host species

Clonality

Isotype

Carrier free

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise