AB703

Anti-Prion protein PrP antibody

Name: Anti-Prion protein PrP antibody (ab703) | Abcam
Brand: Abcam Limited
SKU: AB703
Price: 525 USD
Availability: InStock

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(5 Publications )

Rabbit Polyclonal Prion protein PrP antibody. Suitable for WB and reacts with Mouse samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Cow PRNP.

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Western blot - Anti-Prion protein PrP antibody (AB703)

Lane 1 : MW standard
Lane 2 : wild-type mice (PrPc)
Lane 3 : CDI/PrPsc (PK-)
Lane 4 : CDI/PrPsc (PK+),
Lane 5 : PrP 0/0 mice
CDI = clinically ill CD-1 mice, PK = digested with Proteinase K.

All lanes:

Western blot - Anti-Prion protein PrP antibody (ab703)

Predicted band size: 28 kDa

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This image is courtesy of Dr. M. Glatzel, Institut of Neuropathology, University Hospital of Zürich, Switzerland

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Cow PRNP.

P10279

Specificity

Recognises natural PrPc and conformationally changed PrPc (PrPsc). Recognises recombinant protein ,ab753, in western blot and ELISA.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Preservative: 0.02% Sodium azide Constituents: Whole serum

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prion protein also known as PrP or major prion protein plays a mechanical role in the normal functioning of brain cells. It is a glycoprotein with a flexible structure and has an approximate mass of 35-36 kDa. PrP expression is high in nervous tissue. It is present in neurons and glial cells but also surfaces in other tissues like heart and kidney. Alternate names like p-pr-p and f89 refer to specific conformations or studies related to its structure.

Biological function summary

Prion protein assists in maintaining normal cell activities. Researchers do not fully understand its exact biological role but it might be involved in copper ion uptake and protection against oxidative stress. PrP can form complexes with other cellular proteins some of which help in routing signals inside the cell. Additionally prion protein may have synaptic functions related to neurodevelopment and neuroprotection.

Pathways

Prion protein links to both neuroprotective and neurodegenerative pathways. It participates in signaling pathways that protect neurons from apoptosis. This protein associates closely with copper-dependent pathways possibly related to its capacity to bind copper ions which affects oxidative stress responses. Prion protein also interacts with proteins like synapsin to modulate synaptic transmission.

Prion protein is directly related to prion diseases such as Creutzfeldt-Jakob disease and kuru. These diseases arise from misfolded forms of PrP which aggregate and cause neurodegeneration. The misfolded form referred to as PrP^Sc can induce normal PrP to misfold propagating disease. Dopamine receptor proteins and synaptic proteins can indirectly interact or be affected in these disorders highlighting a complex network of affected neural functions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Its primary physiological function is unclear. May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May promote myelin homeostasis through acting as an agonist for ADGRG6 receptor. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro) (By similarity). Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts. Also provides Cu(2+) or Zn(2+) for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains (PubMed : 12732622, PubMed : 16492732, PubMed : 19242475, PubMed : 19568430).

See full target information Prnp

Alternative Names

CD230, Prn-p, Prp, Prnp, Major prion protein, PrP, PrP27-30, PrP33-35C

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Publications (5)

Recent publications for all applications. Explore the full listand refine your search

BMC cancer 24:1539 PubMed39695426

2024

Prion protein regulates invasiveness in glioblastoma stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mariana B Prado,Bárbara P Coelho,Rebeca P Iglesia,Rodrigo N Alves,Jacqueline M Boccacino,Camila F L Fernandes,Maria Isabel Melo-Escobar,Shamini Ayyadhury,Mario C Cruz,Tiago G Santos,Flávio H Beraldo,Jue Fan,Frederico M Ferreira,Helder I Nakaya,Marco A M Prado,Vania F Prado,Martin L Duennwald,Marilene H Lopes

Cell stress & chaperones 26:443-452 PubMed33547632

2021

Functional genomics screen identifies proteostasis targets that modulate prion protein (PrP) stability.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer Abrams,Taylor Arhar,Sue Ann Mok,Isabelle R Taylor,Martin Kampmann,Jason E Gestwicki

Molecular biology of the cell 26:3313-28 PubMed26224313

2015

The cellular prion protein PrPc is a partner of the Wnt pathway in intestinal epithelial cells.

Applications

Species

Unspecified reactive species

Laura S Besnier,Philippe Cardot,Barbara Da Rocha,Anthony Simon,Damarys Loew,Christophe Klein,Béatrice Riveau,Michel Lacasa,Caroline Clair,Monique Rousset,Sophie Thenet

Age (Dordrecht, Netherlands) 36:151-65 PubMed23867972

2013

Presence of a neo-epitope and absence of amyloid beta and tau protein in degenerative hippocampal granules of aged mice.

Applications

Unspecified application

Species

Unspecified reactive species

Gemma Manich,Jaume del Valle,Itsaso Cabezón,Antoni Camins,Mercè Pallàs,Carme Pelegrí,Jordi Vilaplana

PloS one 3:e3000 PubMed18714380

2008

The cellular prion protein PrP(c) is involved in the proliferation of epithelial cells and in the distribution of junction-associated proteins.

Applications

IP, IP

Species

Mouse, Human

Etienne Morel,Stéphane Fouquet,Carine Strup-Perrot,Cathy Pichol Thievend,Cathy Pichol Thievend,Constance Petit,Damarys Loew,Anne-Marie Faussat,Lucile Yvernault,Martine Pinçon-Raymond,Jean Chambaz,Monique Rousset,Sophie Thenet,Caroline Clair

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Prion protein PrP antibody

Western blot - Anti-Prion protein PrP antibody (AB703)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (5)

Product promise