Anti-Prion protein PrP antibody [EP1802Y]

6 product images

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  Western blot - Anti-Prion protein PrP antibody [EP1802Y] (ab52604)

  The molecular weights observed represent different glycosation states and are consistent with what has been described in the literature (PMID: 20670940, PMID: 19568430, PMID: 15240877 and PMID: 22558368).
  

  Blocking/Diluting buffer: 5% NFDM/TBST

  All lanes: Western blot - Anti-Prion protein PrP antibody [EP1802Y] (ab52604) at 1/5000 dilution

  Lane 1: Human brain lysate at 20 µg

  Lane 2: Mouse brain lysate at 20 µg

  Lane 3: Rat brain lysate at 20 µg

  Lane 4: SK-MEL-28 (Human malignant melanoma ) whole cell lysate at 20 µg

  Lane 5: Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

  Secondary

  All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

  Predicted band size: 28 kDa

  Observed band size: 20-37 kDa

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prion protein PrP antibody [EP1802Y] (ab52604)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling Prion protein PrP with purified ab52604 at 1/500 dilution (1.32 µg/mL). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

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  Western blot - Anti-Prion protein PrP antibody [EP1802Y] (ab52604)

  This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab52604 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

  All lanes: Western blot - Anti-Prion protein PrP antibody [EP1802Y] (ab52604) at 1/5000 dilution

  All lanes: Recombinant Mouse Prion protein PrP (ab74056) at 0.01 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 28 kDa

  Exposure time: 10s

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prion protein PrP antibody [EP1802Y] (ab52604)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling Prion protein PrP with purified ab52604 at 1/500 dilution (1.32 µg/mL). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prion protein PrP antibody [EP1802Y] (ab52604)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling Prion protein PrP with purified ab52604 at 1/500 dilution (1.32 µg/mL). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prion protein PrP antibody [EP1802Y] (ab52604)

  Immunohistochemical analysis of brain glioma using ab52604 (unpurified) at a dilution of 1/100. Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.