Rabbit Recombinant Monoclonal Prion protein PrP antibody. Carrier free. Suitable for IHC-P, WB and reacts with Rat, Mouse, Human, Recombinant full length protein - Mouse samples.
Images
![Western blot - Anti-Prion protein PrP antibody [EP1802Y] - BSA and Azide free (AB238428), expandable thumbnail](https://content.abcam.com/products/images/prion-protein-prp-antibody-ep1802y-bsa-and-azide-free-ab238428--western-blot-img7817.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Prion protein PrP antibody [EP1802Y] - BSA and Azide free (AB238428)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prion protein PrP antibody [EP1802Y] - BSA and Azide free (AB238428), expandable thumbnail](https://content.abcam.com/products/images/prion-protein-prp-antibody-ep1802y-bsa-and-azide-free-ab238428--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img19345.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prion protein PrP antibody [EP1802Y] - BSA and Azide free (AB238428)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prion protein PrP antibody [EP1802Y] - BSA and Azide free (AB238428), expandable thumbnail](https://content.abcam.com/products/images/prion-protein-prp-antibody-ep1802y-bsa-and-azide-free-ab238428--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135915.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prion protein PrP antibody [EP1802Y] - BSA and Azide free (AB238428)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prion protein PrP antibody [EP1802Y] - BSA and Azide free (AB238428), expandable thumbnail](https://content.abcam.com/products/images/prion-protein-prp-antibody-ep1802y-bsa-and-azide-free-ab238428--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img56629.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prion protein PrP antibody [EP1802Y] - BSA and Azide free (AB238428)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prion protein PrP antibody [EP1802Y] - BSA and Azide free (AB238428), expandable thumbnail](https://content.abcam.com/products/images/prion-protein-prp-antibody-ep1802y-bsa-and-azide-free-ab238428--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134852.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prion protein PrP antibody [EP1802Y] - BSA and Azide free (AB238428)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | Flow Cyt (Intra) | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Not recommended |
| Mouse | Tested | Tested | Not recommended | Not recommended |
| Rat | Tested | Tested | Not recommended | Not recommended |
| Recombinant full length protein - Mouse | Not recommended | Tested | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant full length protein - Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant full length protein - Mouse | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant full length protein - Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
5 products for Alternative Version
Target data
Function
Its primary physiological function is unclear. May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May promote myelin homeostasis through acting as an agonist for ADGRG6 receptor. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro) (By similarity). Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts. Also provides Cu(2+) or Zn(2+) for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains (By similarity).
Alternative names
CD230, ALTPRP, PRIP, PRP, PRNP, Major prion protein, PrP, ASCR, PrP27-30, PrP33-35C
Recommended products
Rabbit Recombinant Monoclonal Prion protein PrP antibody. Carrier free. Suitable for IHC-P, WB and reacts with Rat, Mouse, Human, Recombinant full length protein - Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EP1802Y
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab238428 is the carrier-free version of Anti-Prion protein PrP antibody [EP1802Y] ab52604.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Prion protein also known as PrP or major prion protein plays a mechanical role in the normal functioning of brain cells. It is a glycoprotein with a flexible structure and has an approximate mass of 35-36 kDa. PrP expression is high in nervous tissue. It is present in neurons and glial cells but also surfaces in other tissues like heart and kidney. Alternate names like p-pr-p and f89 refer to specific conformations or studies related to its structure.
- Biological function summary
Prion protein assists in maintaining normal cell activities. Researchers do not fully understand its exact biological role but it might be involved in copper ion uptake and protection against oxidative stress. PrP can form complexes with other cellular proteins some of which help in routing signals inside the cell. Additionally prion protein may have synaptic functions related to neurodevelopment and neuroprotection.
- Pathways
Prion protein links to both neuroprotective and neurodegenerative pathways. It participates in signaling pathways that protect neurons from apoptosis. This protein associates closely with copper-dependent pathways possibly related to its capacity to bind copper ions which affects oxidative stress responses. Prion protein also interacts with proteins like synapsin to modulate synaptic transmission.
- Associated diseases and disorders
Prion protein is directly related to prion diseases such as Creutzfeldt-Jakob disease and kuru. These diseases arise from misfolded forms of PrP which aggregate and cause neurodegeneration. The misfolded form referred to as PrP^Sc can induce normal PrP to misfold propagating disease. Dopamine receptor proteins and synaptic proteins can indirectly interact or be affected in these disorders highlighting a complex network of affected neural functions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-Prion protein PrP antibody [EP1802Y] - BSA and Azide free (ab238428), expandable thumbnail](https://content.abcam.com/products/images/prion-protein-prp-antibody-ep1802y-bsa-and-azide-free-ab238428--western-blot-img7817.jpg "Western blot - Anti-Prion protein PrP antibody [EP1802Y] - BSA and Azide free (ab238428)")
Western blot - Anti-Prion protein PrP antibody [EP1802Y] - BSA and Azide free (ab238428)
The molecular weights observed represent different glycosation states and are consistent with what has been described in the literature (PMID: 20670940, PMID: 19568430, PMID: 15240877 and PMID: 22558368).
Blocking/Diluting buffer: 5% NFDM/TBST
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Prion protein PrP antibody [EP1802Y] ab52604).
All lanes: Western blot - Anti-Prion protein PrP antibody [EP1802Y] (Anti-Prion protein PrP antibody [EP1802Y] ab52604) at 1/5000 dilution
Lane 1: Human brain lysate at 20 µg
Lane 2: Mouse brain lysate at 20 µg
Lane 3: Rat brain lysate at 20 µg
Lane 4: SK-MEL-28 (Human malignant melanoma ) whole cell lysate at 20 µg
Lane 5: Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 28 kDa
Observed band size: 20-37 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prion protein PrP antibody [EP1802Y] - BSA and Azide free (ab238428), expandable thumbnail](https://content.abcam.com/products/images/prion-protein-prp-antibody-ep1802y-bsa-and-azide-free-ab238428--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img19345.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prion protein PrP antibody [EP1802Y] - BSA and Azide free (ab238428)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prion protein PrP antibody [EP1802Y] - BSA and Azide free (ab238428)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling Prion protein PrP with purified Anti-Prion protein PrP antibody [EP1802Y] ab52604 at 1/500 dilution (1.32 μg/mL). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Prion protein PrP antibody [EP1802Y] ab52604).![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prion protein PrP antibody [EP1802Y] - BSA and Azide free (ab238428), expandable thumbnail](https://content.abcam.com/products/images/prion-protein-prp-antibody-ep1802y-bsa-and-azide-free-ab238428--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135915.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prion protein PrP antibody [EP1802Y] - BSA and Azide free (ab238428)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prion protein PrP antibody [EP1802Y] - BSA and Azide free (ab238428)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling Prion protein PrP with purified Anti-Prion protein PrP antibody [EP1802Y] ab52604 at 1/500 dilution (1.32 μg/mL). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Prion protein PrP antibody [EP1802Y] ab52604).![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prion protein PrP antibody [EP1802Y] - BSA and Azide free (ab238428), expandable thumbnail](https://content.abcam.com/products/images/prion-protein-prp-antibody-ep1802y-bsa-and-azide-free-ab238428--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img56629.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prion protein PrP antibody [EP1802Y] - BSA and Azide free (ab238428)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prion protein PrP antibody [EP1802Y] - BSA and Azide free (ab238428)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling Prion protein PrP with purified Anti-Prion protein PrP antibody [EP1802Y] ab52604 at 1/500 dilution (1.32 μg/mL). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Prion protein PrP antibody [EP1802Y] ab52604).![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prion protein PrP antibody [EP1802Y] - BSA and Azide free (ab238428), expandable thumbnail](https://content.abcam.com/products/images/prion-protein-prp-antibody-ep1802y-bsa-and-azide-free-ab238428--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134852.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prion protein PrP antibody [EP1802Y] - BSA and Azide free (ab238428)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prion protein PrP antibody [EP1802Y] - BSA and Azide free (ab238428)
Immunohistochemical analysis of brain glioma using Anti-Prion protein PrP antibody [EP1802Y] ab52604 (unpurified) at a dilution of 1/100. Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Prion protein PrP antibody [EP1802Y] ab52604).
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