AB136919

Anti-Prion protein PrP antibody [T16-R]

Name: Anti-Prion protein PrP antibody [T16-R] (ab136919) | Abcam
Brand: Abcam Limited
SKU: AB136919
Price: 535 USD
Availability: InStock

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(2 Publications)

Rabbit Monoclonal Prion protein PrP antibody. Suitable for IP, WB and reacts with Mouse, Rat, Human, Cow, Sheep samples. Cited in 2 publications.

View Alternative Names

CD230, ALTPRP, PRIP, PRP, PRNP, Major prion protein, PrP, ASCR, PrP27-30, PrP33-35C

2 Images

Supplier Data

Western blot - Anti-Prion protein PrP antibody [T16-R] (AB136919)

All lanes:

Western blot - Anti-Prion protein PrP antibody [T16-R] (ab136919) at 1/1000 dilution

Lane 1:

Prion protein PrP-KO CF10 cell lysate at 30 µg

Lane 2:

Mouse hippocampus tissue lysate at 30 µg

Lane 3:

HEK293 cells transfected with Mouse Prion protein PrP bearing 3F4 epitope at 30 µg

Lane 4:

Recombinant Mouse Prion protein PrP at 30 µg

Predicted band size: 28 kDa

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Supplier Data

Western blot - Anti-Prion protein PrP antibody [T16-R] (AB136919)

All lanes:

Western blot - Anti-Prion protein PrP antibody [T16-R] (ab136919) at 1/1000 dilution

Lane 1:

Mouse brain extract at 200 µg

Lane 2:

HEK293 cells transfected with Human Prion protein PrP gene at 200 µg

Lane 3:

HEK293 cells transfected with empty vector at 200 µg

Predicted band size: 28 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

T16-R

Isotype

IgG

Carrier free

Reacts with

Mouse, Human, Sheep, Cow, Rat

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Epitope

Antibody recognizes the epitope located between Thr33 - Gly46

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "Incubate the membrane with antibody diluted in blocking buffer 2 hours at room temperature." }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "Incubate the membrane with antibody diluted in blocking buffer 2 hours at room temperature." }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This immunoglobulin is the product of one single B-cell line from the crude anti-peptide polyclonal anti-serum. This antibody is purified using a proprietary technique and offers a completely post-translationally modified and properly glycosylated antibody. This offers increased stability.

Storage buffer

pH: 8 Preservative: 0.05% Sodium azide Constituents: 1% BSA, 0.32% Tris HCl

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prion protein also known as PrP or major prion protein plays a mechanical role in the normal functioning of brain cells. It is a glycoprotein with a flexible structure and has an approximate mass of 35-36 kDa. PrP expression is high in nervous tissue. It is present in neurons and glial cells but also surfaces in other tissues like heart and kidney. Alternate names like p-pr-p and f89 refer to specific conformations or studies related to its structure.

Biological function summary

Prion protein assists in maintaining normal cell activities. Researchers do not fully understand its exact biological role but it might be involved in copper ion uptake and protection against oxidative stress. PrP can form complexes with other cellular proteins some of which help in routing signals inside the cell. Additionally prion protein may have synaptic functions related to neurodevelopment and neuroprotection.

Pathways

Prion protein links to both neuroprotective and neurodegenerative pathways. It participates in signaling pathways that protect neurons from apoptosis. This protein associates closely with copper-dependent pathways possibly related to its capacity to bind copper ions which affects oxidative stress responses. Prion protein also interacts with proteins like synapsin to modulate synaptic transmission.

Prion protein is directly related to prion diseases such as Creutzfeldt-Jakob disease and kuru. These diseases arise from misfolded forms of PrP which aggregate and cause neurodegeneration. The misfolded form referred to as PrP^Sc can induce normal PrP to misfold propagating disease. Dopamine receptor proteins and synaptic proteins can indirectly interact or be affected in these disorders highlighting a complex network of affected neural functions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Its primary physiological function is unclear. May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May promote myelin homeostasis through acting as an agonist for ADGRG6 receptor. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro) (By similarity). Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts. Also provides Cu(2+) or Zn(2+) for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains (By similarity).

See full target information PRNP

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Regenerative biomaterials 12:rbaf089 PubMed41048976

2025

ROS scavenging MnO nanozyme regulated immune microenvironment and affects intercellular interaction to promote wound healing in diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuoyuan Li,Ao Zheng,Chen Liang,Zhiyuan Mao,Tanjun Deng,Lingyan Cao,Chen Wang

FASEB journal : official publication of the Federa 31:2785-2796 PubMed28314768

2017

infection liberates transmissible, cytotoxic prion amyloids.

Applications

Unspecified application

Species

Unspecified reactive species

Ron Balczon,K Adam Morrow,Chun Zhou,Bradley Edmonds,Mikhail Alexeyev,Jean-Francois Pittet,Brant M Wagener,Stephen A Moser,Silas Leavesley,Xiangming Zha,Dara W Frank,Troy Stevens

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Prion protein PrP antibody [T16-R]

Western blot - Anti-Prion protein PrP antibody [T16-R] (AB136919)

Western blot - Anti-Prion protein PrP antibody [T16-R] (AB136919)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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