AB108385

Anti-PRKACG antibody [EP2647Y]

Name: Anti-PRKACG antibody [EP2647Y] (ab108385) | Abcam
Brand: Abcam Limited
SKU: AB108385
Price: 340 USD
Availability: InStock
Rating: 3 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(9 Publications)

Rabbit Recombinant Monoclonal KAPCG antibody. Suitable for WB and reacts with Human samples. Cited in 9 publications.

View Alternative Names

cAMP-dependent protein kinase catalytic subunit gamma, PKA C-gamma, PRKACG

1 Images

Supplier Data

Western blot - Anti-PRKACG antibody [EP2647Y] (AB108385)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PRKACG antibody [EP2647Y] (ab108385) at 1/10000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Lane 2:

PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 40 kDa

false

Exposure time: 40s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2647Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is likely to react with the alpha subunit, as it possesses 85.7% similar homology with the immunogen. Additionally, it may react with the beta subunit, possessing 78.6% homology.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

The production method for this product has been changed from hybridoma to recombinant on 27th September 2024.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PRKACG also known as Protein Kinase Catalytic Subunit Gamma or cAMP-dependent protein kinase catalytic subunit gamma is a protein involved in kinase activity. This protein has a molecular mass of approximately 40 kDa. PRKACG is expressed in several tissues with notably high expression levels in the brain and testis. It belongs to the protein kinase family and serves a role in transferring phosphate groups from ATP to specific serine or threonine residues on substrate proteins.

Biological function summary

This kinase plays a part in various cellular signaling processes particularly in the regulation of metabolism gene expression and cell differentiation. PRKACG forms part of the larger protein kinase A (PKA) complex which includes regulatory and additional catalytic subunits. This enables precise modulation of cellular responses to different signals important for maintaining homeostasis and responding to environmental changes.

Pathways

PRKACG participates in the cAMP signaling pathway and the long-term depression pathway. In the cAMP signaling pathway it acts with proteins like PRKAR1A and PRKAR2A which serve as regulatory subunits to transmit signals that affect cellular functions. The activation of PRKACG by cAMP leads to phosphorylation events that can change the activity of enzymes involved in energy metabolism and other cellular processes.

PRKACG has connections to psychiatric disorders such as schizophrenia and neurological conditions like Huntington's disease. Alterations in cAMP signaling where PRKACG plays a role can link to these diseases. The interplay between PRKACG and proteins like PRKAR1A may disrupt cellular signaling contributing to the pathophysiology of these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Phosphorylates a large number of substrates in the cytoplasm and the nucleus.

See full target information cAMP-dependent protein kinase catalytic subunit gamma

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Translational neurodegeneration 14:43 PubMed40847374

2025

Cyclooxygenase-1 deletion in 5 × FAD mice protects against microglia-induced neuroinflammation and mitigates cognitive impairment.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Wang,Hong Ni,Yu Wang,Luyao Wei,Hanqing Ding,Zhongzhao Guo,Hao Pan,Ying Yu,Jia Luo,Weidong Pan,Deheng Wang,Zun-Ji Ke

Clinical science (London, England : 1979) 138:1481-1502 PubMed39432712

2024

Placental small extracellular vesicles from normal pregnancy and gestational diabetes increase insulin gene transcription and content in β cells.

Applications

Unspecified application

Species

Unspecified reactive species

Faheem Seedat,Neva Kandzija,Michael J Ellis,Shuhan Jiang,Asselzhan Sarbalina,James Bancroft,Edward Drydale,Svenja S Hester,Roman Fischer,Alisha N Wade,M Irina Stefana,John A Todd,Manu Vatish

Communications biology 5:680 PubMed35804021

2022

Eleutheroside E supplementation prevents radiation-induced cognitive impairment and activates PKA signaling via gut microbiota.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Song,Fangyuan Duan,Ting Ju,Yue Qin,Deyong Zeng,Shan Shan,Yudong Shi,Yingchun Zhang,Weihong Lu

Nature communications 11:5433 PubMed33116136

2020

Interleukin-1 receptor-induced PGE production controls acetylcholine-mediated cardiac dysfunction and mortality during scorpion envenomation.

Applications

Unspecified application

Species

Unspecified reactive species

Mouzarllem B Reis,Fernanda L Rodrigues,Natalia Lautherbach,Alexandre Kanashiro,Carlos A Sorgi,Alyne F G Meirelles,Carlos A A Silva,Karina F Zoccal,Camila O S Souza,Simone G Ramos,Alessandra K Matsuno,Lenaldo B Rocha,Helio C Salgado,Luiz C C Navegantes,Ísis C Kettelhut,Palmira Cupo,Luiz G Gardinassi,Lúcia H Faccioli

Aging cell 19:e13055 PubMed31668016

2019

Tau inhibits PKA by nuclear proteasome-dependent PKAR2α elevation with suppressed CREB/GluA1 phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Jinwang Ye,Yaling Yin,Huanhuan Liu,Lin Fang,Xiaoqing Tao,Linyu Wei,Yue Zuo,Ying Yin,Dan Ke,Jian-Zhi Wang

PloS one 14:e0214606 PubMed30990811

2019

PID1 alters the antilipolytic action of insulin and increases lipolysis via inhibition of AKT/PKA pathway activation.

Applications

Unspecified application

Species

Unspecified reactive species

Chunyan Yin,Wei Hua Liu,Yuesheng Liu,Li Wang,Yanfeng Xiao

Cellular physiology and biochemistry : internation 45:1023-1033 PubMed29439250

2018

Hoxa5 Promotes Adipose Differentiation via Increasing DNA Methylation Level and Inhibiting PKA/HSL Signal Pathway in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Weina Cao,Yatao Xu,Dan Luo,Muhammad Saeed,Chao Sun

Cell death & disease 8:e2996 PubMed28796260

2017

Clinical-grade human umbilical cord-derived mesenchymal stem cells reverse cognitive aging via improving synaptic plasticity and endogenous neurogenesis.

Applications

Species

Unspecified reactive species

Ning Cao,Tuling Liao,Jiajing Liu,Zeng Fan,Quan Zeng,Junnian Zhou,Haiyun Pei,Jiafei Xi,Lijuan He,Lin Chen,Xue Nan,Yali Jia,Wen Yue,Xuetao Pei

Oncotarget 8:60135-60148 PubMed28947959

2017

ColXV promotes adipocyte differentiation via inhibiting DNA methylation and cAMP/PKA pathway in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Guannv Liu,Meihang Li,Yatao Xu,Song Wu,Muhammad Saeed,Chao Sun

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PRKACG antibody [EP2647Y]

Western blot - Anti-PRKACG antibody [EP2647Y] (AB108385)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise