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AB284776

Anti-PRKACG antibody [EP2647Y] - BSA and Azide free

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Rabbit Recombinant Monoclonal KAPCG antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

cAMP-dependent protein kinase catalytic subunit gamma, PKA C-gamma, PRKACG

1 Images
Western blot - Anti-PRKACG antibody [EP2647Y] - BSA and Azide free (AB284776)
  • WB

Supplier Data

Western blot - Anti-PRKACG antibody [EP2647Y] - BSA and Azide free (AB284776)

This data was developed using ab108385, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PRKACG antibody [EP2647Y] (<a href='/en-us/products/primary-antibodies/prkacg-antibody-ep2647y-ab108385'>ab108385</a>) at 1/10000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Lane 2:

PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 40 kDa

false

Exposure time: 40s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2647Y

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is likely to react with the alpha subunit, as it possesses 85.7% similar homology with the immunogen. Additionally, it may react with the beta subunit, possessing 78.6% homology.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

ab284776 is the carrier-free version of ab108385.

<p>The production method for this product has been changed from hybridoma to recombinant on 27th September 2024.</p>

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PRKACG also known as Protein Kinase Catalytic Subunit Gamma or cAMP-dependent protein kinase catalytic subunit gamma is a protein involved in kinase activity. This protein has a molecular mass of approximately 40 kDa. PRKACG is expressed in several tissues with notably high expression levels in the brain and testis. It belongs to the protein kinase family and serves a role in transferring phosphate groups from ATP to specific serine or threonine residues on substrate proteins.
Biological function summary

This kinase plays a part in various cellular signaling processes particularly in the regulation of metabolism gene expression and cell differentiation. PRKACG forms part of the larger protein kinase A (PKA) complex which includes regulatory and additional catalytic subunits. This enables precise modulation of cellular responses to different signals important for maintaining homeostasis and responding to environmental changes.

Pathways

PRKACG participates in the cAMP signaling pathway and the long-term depression pathway. In the cAMP signaling pathway it acts with proteins like PRKAR1A and PRKAR2A which serve as regulatory subunits to transmit signals that affect cellular functions. The activation of PRKACG by cAMP leads to phosphorylation events that can change the activity of enzymes involved in energy metabolism and other cellular processes.

PRKACG has connections to psychiatric disorders such as schizophrenia and neurological conditions like Huntington's disease. Alterations in cAMP signaling where PRKACG plays a role can link to these diseases. The interplay between PRKACG and proteins like PRKAR1A may disrupt cellular signaling contributing to the pathophysiology of these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Phosphorylates a large number of substrates in the cytoplasm and the nucleus.
See full target information cAMP-dependent protein kinase catalytic subunit gamma
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com