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AB233561

Anti-PRKAG3 antibody [1C5D10]

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Mouse Monoclonal PRKAG3 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PRKAG3 aa 1-200.

View Alternative Names

AMPKG3, PRKAG3, 5'-AMP-activated protein kinase subunit gamma-3, AMPK gamma3, AMPK subunit gamma-3

2 Images
Western blot - Anti-PRKAG3 antibody [1C5D10] (AB233561)
  • WB

Supplier Data

Western blot - Anti-PRKAG3 antibody [1C5D10] (AB233561)

All lanes:

Western blot - Anti-PRKAG3 antibody [1C5D10] (ab233561) at 1/500 dilution

Lane 1:

HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate

Lane 2:

PRKAG3 (amino acids 9-151) - hIgGFc transfected HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate

Predicted band size: 54 kDa

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Western blot - Anti-PRKAG3 antibody [1C5D10] (AB233561)
  • WB

Supplier Data

Western blot - Anti-PRKAG3 antibody [1C5D10] (AB233561)

(Expected MW is 41.1 kDa)

All lanes:

Western blot - Anti-PRKAG3 antibody [1C5D10] (ab233561) at 1/500 dilution

All lanes:

Recombinant human PRKAG3 protein (amino acids 9-151)

Predicted band size: 54 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1C5D10

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human PRKAG3 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q9UGI9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from TCS.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PRKAG3 also known as AMP-activated protein kinase subunit gamma-3 is a regulatory subunit of the AMP-activated protein kinase (AMPK) complex. The gamma-3 subunit contributes to the full AMPK function particularly its sensitivity to energy status changes within cells. This subunit weighs approximately 38.1 kDa and is mainly expressed in skeletal muscle tissue. It plays an important role in cellular energy homeostasis by responding to depletion in intracellular ATP levels.
Biological function summary

PRKAG3 interacts with AMP and ADP which activates AMPK when energy levels are low. It forms a complex with two other subunits the alpha (catalytic) and beta (regulatory) subunits to become fully active. Once activated AMPK executes various cellular functions including the regulation of glucose uptake and fatty acid oxidation primarily within muscle tissues which is essential for maintaining energy balance.

Pathways

PRKAG3 takes part in the AMPK signaling pathway a central regulator of metabolic pathways that maintain cellular energy balance. It connects to glucose and lipid metabolic processes interacting with proteins like ACC (acetyl-CoA carboxylase) for fatty acid synthesis inhibition and GLUT4 (glucose transporter type 4) for glucose uptake enhancement. Through these interactions PRKAG3 influences critical metabolic adaptations to energy stress.

Mutations or dysregulation in PRKAG3 have links with glycogen storage disease type V (McArdle disease) and insulin resistance. In particular disorders in glycemic control connect this protein to the regulation of glucose metabolism. PRKAG3 mutations can affect the typical glycogen synthesis and breakdown in skeletal muscle impacting energy availability during exercise. Additionally its activity has associations with insulin-signaling networks in conjunction with proteins like insulin receptor substrate-1 (IRS-1) impacting glucose homeostasis and insulin sensitivity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism (PubMed : 14722619, PubMed : 17878938, PubMed : 24563466). In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes : inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. AMPK also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. The AMPK gamma3 subunit is a non-catalytic subunit with a regulatory role in muscle energy metabolism (PubMed : 17878938). It mediates binding to AMP, ADP and ATP, leading to AMPK activation or inhibition : AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.
See full target information PRKAG3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB203214

Anti-PRKAG3 antibody

primary-antibodies

prkag3-antibody-ab203214

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