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AB314003

Anti-PRKAR1A antibody [1C3]

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Rabbit Monoclonal PRKAR1A antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human PRKAR1A.

View Alternative Names

PKR1, PRKAR1, TSE1, PRKAR1A, cAMP-dependent protein kinase type I-alpha regulatory subunit, Tissue-specific extinguisher 1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-PRKAR1A antibody [1C3] (AB314003)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PRKAR1A antibody [1C3] (AB314003)

Immunofluorescence staining of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells with ab314003 at 1 : 36, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).

Western blot - Anti-PRKAR1A antibody [1C3] (AB314003)
  • WB

Supplier Data

Western blot - Anti-PRKAR1A antibody [1C3] (AB314003)

All lanes:

Western blot - Anti-PRKAR1A antibody [1C3] (ab314003) at 1.1 µg/mL

All lanes:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Observed band size: 43 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

1C3

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human PRKAR1A.

P10644

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/20 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein PRKAR1A also known as Protein Kinase A (PKA) type I-alpha regulatory subunit acts as a regulatory subunit of the cAMP-dependent protein kinase (PKA) enzyme. It exhibits an approximate mass of 42 kDa. PRKAR1A plays a role in modulating the enzyme's activity by binding to cAMP which causes the dissociation of the regulatory subunit and activation of the catalytic subunit. PRKAR1A is broadly expressed in many tissues including the thyroid heart and adrenal glands indicating its widespread physiological importance.
Biological function summary

The regulatory subunit type I-alpha influences processes by deactivating the catalytic subunit to maintain appropriate levels of enzyme activity. It engages in forming a functional holoenzyme complex with the catalytic subunits of PKA. This mechanistic interaction impacts various cellular functions such as cell growth metabolism and gene transcription highlighting the broad biological impact of PRKAR1A.

Pathways

The protein PRKAR1A plays a role in the cAMP signaling pathway and the MAPK/ERK pathway. In the cAMP pathway it partners with the catalytic subunits of PKA to regulate cellular responses to fluctuating levels of cAMP. The protein links with other PKA subunits and signaling molecules such as CREB to mediate physiological responses to extracellular stimuli therefore interfacing with multifaceted cellular pathways.

Alterations in PRKAR1A function are connected to Carney Complex a hereditary disease characterized by cardiac myxomas skin abnormalities and endocrine tumors. Mutations in PRKAR1A contribute to endocrine-related disorders like Cushing's syndrome. The protein relates to other proteins such as PKA catalytic subunits which may also undergo dysregulation highlighting PRKAR1A's influence in disease pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells.
See full target information PRKAR1A
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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