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AB60064

Anti-PRKAR1A antibody [3546C2a]

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(2 Publications)

Mouse Monoclonal PRKAR1A antibody. Suitable for WB and reacts with Recombinant full length protein - Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PRKAR1A.

View Alternative Names

PKR1, PRKAR1, TSE1, PRKAR1A, cAMP-dependent protein kinase type I-alpha regulatory subunit, Tissue-specific extinguisher 1

1 Images
Western blot - Anti-PRKAR1A antibody [3546C2a] (AB60064)
  • WB

Unknown

Western blot - Anti-PRKAR1A antibody [3546C2a] (AB60064)

Western Blot analysis of immunized recombinant protein, using ab60064

All lanes:

Western blot - Anti-PRKAR1A antibody [3546C2a] (ab60064)

Predicted band size: 43 kDa

Observed band size: 43 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3546C2a

Isotype

IgG1

Carrier free

No

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human PRKAR1A.

P10644

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified using protein G column chromatography, from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.03% Tripotassium orthophosphate, 0.0225% Potassium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein PRKAR1A also known as Protein Kinase A (PKA) type I-alpha regulatory subunit acts as a regulatory subunit of the cAMP-dependent protein kinase (PKA) enzyme. It exhibits an approximate mass of 42 kDa. PRKAR1A plays a role in modulating the enzyme's activity by binding to cAMP which causes the dissociation of the regulatory subunit and activation of the catalytic subunit. PRKAR1A is broadly expressed in many tissues including the thyroid heart and adrenal glands indicating its widespread physiological importance.
Biological function summary

The regulatory subunit type I-alpha influences processes by deactivating the catalytic subunit to maintain appropriate levels of enzyme activity. It engages in forming a functional holoenzyme complex with the catalytic subunits of PKA. This mechanistic interaction impacts various cellular functions such as cell growth metabolism and gene transcription highlighting the broad biological impact of PRKAR1A.

Pathways

The protein PRKAR1A plays a role in the cAMP signaling pathway and the MAPK/ERK pathway. In the cAMP pathway it partners with the catalytic subunits of PKA to regulate cellular responses to fluctuating levels of cAMP. The protein links with other PKA subunits and signaling molecules such as CREB to mediate physiological responses to extracellular stimuli therefore interfacing with multifaceted cellular pathways.

Alterations in PRKAR1A function are connected to Carney Complex a hereditary disease characterized by cardiac myxomas skin abnormalities and endocrine tumors. Mutations in PRKAR1A contribute to endocrine-related disorders like Cushing's syndrome. The protein relates to other proteins such as PKA catalytic subunits which may also undergo dysregulation highlighting PRKAR1A's influence in disease pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells.
See full target information PRKAR1A
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

eLife 6: PubMed28079521

2017

Isoform-specific subcellular localization and function of protein kinase A identified by mosaic imaging of mouse brain.

Applications

Unspecified application

Species

Unspecified reactive species

Ronit Ilouz,Varda Lev-Ram,Eric A Bushong,Travis L Stiles,Dinorah Friedmann-Morvinski,Christopher Douglas,Jeffrey L Goldberg,Mark H Ellisman,Susan S Taylor

Chembiochem : a European journal of chemical biology 11:963-71 PubMed20394097

2010

Sphingosine kinase interacting protein is an A-kinase anchoring protein specific for type I cAMP-dependent protein kinase.

Applications

Unspecified application

Species

Unspecified reactive species

Duangnapa Kovanich,Marcel A G van der Heyden,Thin Thin Aye,Toon A B van Veen,Albert J R Heck,Arjen Scholten
View all publications
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Product promise

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