AB248879

Anti-PRKAR1A (phospho S77) antibody [EPMAYR1-127] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal PRKAR1A phospho S77 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

PKR1, PRKAR1, TSE1, PRKAR1A, cAMP-dependent protein kinase type I-alpha regulatory subunit, Tissue-specific extinguisher 1

1 Images

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Western blot - Anti-PRKAR1A (phospho S77) antibody [EPMAYR1-127] - BSA and Azide free (AB248879)

This data was developed using ab139682, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PRKAR1A (phospho S77) antibody [EPMAYR1-127] (<a href='/en-us/products/primary-antibodies/prkar1a-phospho-s77-antibody-epmayr1-127-ab139682'>ab139682</a>) at 1/1000 dilution

Lane 1:

Fetal heart lysate at 10 µg

Lane 2:

Fetal heart lysate treated with Alkaline Phosphatase at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 43 kDa

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Unconjugated

Anti-PRKAR1A (phospho S77) antibody [EPMAYR1-127]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPMAYR1-127

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody only detects Protein Kinase A regulatory subunit I alpha/PRKAR1A phosphorylated at serine 77.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab248879 is the carrier-free version of ab139682.

This antibody was developed as part of a collaboration between Abcam and the lab of Dr. Ozgur Ogut at the Mayo Clinic.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein PRKAR1A also known as Protein Kinase A (PKA) type I-alpha regulatory subunit acts as a regulatory subunit of the cAMP-dependent protein kinase (PKA) enzyme. It exhibits an approximate mass of 42 kDa. PRKAR1A plays a role in modulating the enzyme's activity by binding to cAMP which causes the dissociation of the regulatory subunit and activation of the catalytic subunit. PRKAR1A is broadly expressed in many tissues including the thyroid heart and adrenal glands indicating its widespread physiological importance.

Biological function summary

The regulatory subunit type I-alpha influences processes by deactivating the catalytic subunit to maintain appropriate levels of enzyme activity. It engages in forming a functional holoenzyme complex with the catalytic subunits of PKA. This mechanistic interaction impacts various cellular functions such as cell growth metabolism and gene transcription highlighting the broad biological impact of PRKAR1A.

Pathways

The protein PRKAR1A plays a role in the cAMP signaling pathway and the MAPK/ERK pathway. In the cAMP pathway it partners with the catalytic subunits of PKA to regulate cellular responses to fluctuating levels of cAMP. The protein links with other PKA subunits and signaling molecules such as CREB to mediate physiological responses to extracellular stimuli therefore interfacing with multifaceted cellular pathways.

Alterations in PRKAR1A function are connected to Carney Complex a hereditary disease characterized by cardiac myxomas skin abnormalities and endocrine tumors. Mutations in PRKAR1A contribute to endocrine-related disorders like Cushing's syndrome. The protein relates to other proteins such as PKA catalytic subunits which may also undergo dysregulation highlighting PRKAR1A's influence in disease pathogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells.

See full target information PRKAR1A phospho S77

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of personalized medicine 12: PubMed35207643

2022

Detyrosinated α-Tubulin, Vimentin and PD-L1 in Circulating Tumor Cells (CTCs) Isolated from Non-Small Cell Lung Cancer (NSCLC) Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Spyridoula D Katsarou,Ippokratis Messaritakis,Anastasia Voumvouraki,Stavros Kakavogiannis,Athanasios Κotsakis,Saad Alkahtani,Christos Stournaras,Stuart S Martin,Vassilis Georgoulias,Galatea Kallergi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PRKAR1A (phospho S77) antibody [EPMAYR1-127] - BSA and Azide free

Western blot - Anti-PRKAR1A (phospho S77) antibody [EPMAYR1-127] - BSA and Azide free (AB248879)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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