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AB167325

Anti-PRKAR1B antibody

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(1 Publication)

Mouse Polyclonal PRKAR1B antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human cAMP-dependent protein kinase type I-beta regulatory subunit.

View Alternative Names

cAMP-dependent protein kinase type I-beta regulatory subunit, PRKAR1B

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-PRKAR1B antibody (AB167325)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PRKAR1B antibody (AB167325)

Immunofluorescent analysis of PRKAR1B expression in HeLa cells using ab167325 at 10 μg/ml dilution.

Western blot - Anti-PRKAR1B antibody (AB167325)
  • WB

Unknown

Western blot - Anti-PRKAR1B antibody (AB167325)

All lanes:

Western blot - Anti-PRKAR1B antibody (ab167325) at 1 µg/mL

Lane 1:

PRKAR1B transfected 293T cell lysate at 50 µg

Lane 2:

Non transfected 293T cell lysate at 50 µg

Secondary

All lanes:

Goat Anti-Mouse IgG HRP at 1/2500 dilution

Predicted band size: 43 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human cAMP-dependent protein kinase type I-beta regulatory subunit.

P31321

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Primary Antibodies

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  • 0
  • 0
Primary Antibodies

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PRKAR1B also known as the regulatory subunit type I beta of protein kinase A (PKA) plays an important role in the regulation of cAMP-dependent PKA activity. This protein has a molecular mass of approximately 44 kDa. It binds to the catalytic subunit of PKA inhibiting its activity until cAMP binds which then triggers a conformational change and release of the active catalytic subunit. PRKAR1B is expressed in various tissues with notable abundance in the brain highlighting its significance in neuronal signaling.
Biological function summary

PRKAR1B functions as part of the PKA holoenzyme complex which includes both regulatory and catalytic subunits. The regulatory subunit PRKAR1B directly controls when and where PKA catalytic activity occurs influencing multiple cellular responses. This protein modulates processes like glycogen lipid and protein metabolism by contributing to the precise regulation of cAMP signaling pathways. Its activity affects a range of fundamental cellular functions indicative of its presence in diverse tissues.

Pathways

PRKAR1B is intricately involved in the cAMP signaling pathway and the MAPK/ERK pathway. The cAMP pathway is important for transferring the signals of many hormones and neurotransmitters where PRKAR1B regulates transient PKA activation. In the MAPK/ERK pathway PRKAR1B indirectly influences cellular growth and differentiation by modulating secondary messengers. Proteins such as the catalytic subunits of PKA and CREB a transcription factor interact with PRKAR1B illustrating its central role in cellular responses to external signals.

PRKAR1B has been associated with neurodegenerative diseases like Alzheimer’s and mood disorders such as major depressive disorder. Altered regulation of the cAMP signaling pathway influenced by PRKAR1B affects neuronal survival and function potentially leading to neurodegeneration. Additionally imbalances in PRKAR1B-mediated pathways can contribute to mood disorders. Proteins such as the catalytic subunits of PKA and BDNF (brain-derived neurotrophic factor) interact with PRKAR1B in these contexts indicating its potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells.
See full target information cAMP-dependent protein kinase type I-beta regulatory subunit
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Anti-cancer drugs 27:756-65 PubMed27276402

2016

Antimyeloma activity of bromodomain inhibitors on the human myeloma cell line U266 by downregulation of MYCL.

Applications

Unspecified application

Species

Unspecified reactive species

Kazuhito Suzuki,Kouhei Yamamoto,Yasuhiro Arakawa,Hisashi Yamada,Keisuke Aiba,Masanobu Kitagawa
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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