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AB264220

Anti-PRKAR2 antibody

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Rabbit Polyclonal PKA R2/PKR2 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human PRKAR2A aa 50-100.

View Alternative Names

PKR2, PRKAR2, PRKAR2A, cAMP-dependent protein kinase type II-alpha regulatory subunit

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2 antibody (AB264220)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2 antibody (AB264220)

Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for PRKAR2 using ab264220 at 1/200 dilution in immunohistochemical analysis. Detection : DAB.

Immunoprecipitation - Anti-PRKAR2 antibody (AB264220)
  • IP

Supplier Data

Immunoprecipitation - Anti-PRKAR2 antibody (AB264220)

PRKAR2 was immunoprecipitated from 1 mg HeLa whole cell lysate using ab264220 at 3 μg/mg lysate. Western blot was performed on the immunoprecipitate using ab264220 at 1 μg/ml.

Lane 1 : ab264220 IP in HeLa whole cell lysate.
Lane 2 : Control IgG.

All lanes:

Immunoprecipitation - Anti-PRKAR2 antibody (ab264220)

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Exposure time: 30s

Western blot - Anti-PRKAR2 antibody (AB264220)
  • WB

Supplier Data

Western blot - Anti-PRKAR2 antibody (AB264220)

All lanes:

Western blot - Anti-PRKAR2 antibody (ab264220) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

HEK-293T whole cell lysate at 50 µg

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Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, IP

applications

Immunogen

Synthetic Peptide within Human PRKAR2A aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

P13861

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mL", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "<p>Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PRKAR2 protein also known as the protein kinase cAMP-dependent type II regulatory subunit beta plays an important role in regulating protein kinase A (PKA) activity. This protein has a molecular mass of approximately 46 kDa and is found mainly in human tissues such as the brain liver and heart. PRKAR2 forms a complex with catalytic subunits to modulate PKA's activity when cyclic AMP (cAMP) levels fluctuate. This intricate mechanism allows it to effectively control cellular responses through phosphorylation.
Biological function summary

Protein kinase A activity depends largely on the proper function of PRKAR2. PRKAR2 is part of the larger PKA holoenzyme complex which includes regulatory and catalytic subunits. PRKAR2 when bound to cAMP changes its conformation causing the dissociation of the catalytic subunits leading to their activation. This activation is important for a multitude of biological processes including regulation of glycogen sugar and lipid metabolism as well as cell cycle progression.

Pathways

The signaling related to cAMP is a major pathway where PRKAR2 is active. This protein is related to both the cAMP signaling and Ras signaling pathways. Within these networks the cAMP-mediated activation of PKA impacts the function of proteins such as CREB and MAPK influencing gene transcription and cell survival. The interaction with these pathways highlights the significant influence PRKAR2 has on cellular function and regulation.

PRKAR2 has been linked to certain health conditions like Carney complex and some types of cancer. Alterations in PRKAR2 expression or mutations can lead to dysregulation of PKA activity affecting cell proliferation and survival. The protein's interaction with other complexes such as those involving the protein PRKACA contributes to understanding these conditions. These connections suggest that targeting PRKAR2 could potentially offer therapeutic strategies for related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
See full target information PRKAR2A
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