Rabbit Polyclonal PKA R2/PKR2 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human PRKAR2A aa 50-100.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human PRKAR2A aa 50-100. The exact immunogen used to generate this antibody is proprietary information. Database link P13861
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mL | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/1000.00000 | Notes Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
Target data
Function
Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
Alternative names
PKR2, PRKAR2, PRKAR2A, cAMP-dependent protein kinase type II-alpha regulatory subunit
Recommended products
Rabbit Polyclonal PKA R2/PKR2 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human PRKAR2A aa 50-100.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human PRKAR2A aa 50-100. The exact immunogen used to generate this antibody is proprietary information. Database link P13861
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The PRKAR2 protein also known as the protein kinase cAMP-dependent type II regulatory subunit beta plays an important role in regulating protein kinase A (PKA) activity. This protein has a molecular mass of approximately 46 kDa and is found mainly in human tissues such as the brain liver and heart. PRKAR2 forms a complex with catalytic subunits to modulate PKA's activity when cyclic AMP (cAMP) levels fluctuate. This intricate mechanism allows it to effectively control cellular responses through phosphorylation.
- Biological function summary
Protein kinase A activity depends largely on the proper function of PRKAR2. PRKAR2 is part of the larger PKA holoenzyme complex which includes regulatory and catalytic subunits. PRKAR2 when bound to cAMP changes its conformation causing the dissociation of the catalytic subunits leading to their activation. This activation is important for a multitude of biological processes including regulation of glycogen sugar and lipid metabolism as well as cell cycle progression.
- Pathways
The signaling related to cAMP is a major pathway where PRKAR2 is active. This protein is related to both the cAMP signaling and Ras signaling pathways. Within these networks the cAMP-mediated activation of PKA impacts the function of proteins such as CREB and MAPK influencing gene transcription and cell survival. The interaction with these pathways highlights the significant influence PRKAR2 has on cellular function and regulation.
- Associated diseases and disorders
PRKAR2 has been linked to certain health conditions like Carney complex and some types of cancer. Alterations in PRKAR2 expression or mutations can lead to dysregulation of PKA activity affecting cell proliferation and survival. The protein's interaction with other complexes such as those involving the protein PRKACA contributes to understanding these conditions. These connections suggest that targeting PRKAR2 could potentially offer therapeutic strategies for related diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Immunoprecipitation - Anti-PRKAR2 antibody (ab264220)
PRKAR2 was immunoprecipitated from 1 mg HeLa whole cell lysate using ab264220 at 3 μg/mg lysate. Western blot was performed on the immunoprecipitate using ab264220 at 1 μg/ml.
Lane 1: ab264220 IP in HeLa whole cell lysate.
Lane 2: Control IgG.All lanes: Immunoprecipitation - Anti-PRKAR2 antibody (ab264220)
Developed using the ECL technique.
Exposure time: 30s
Western blot - Anti-PRKAR2 antibody (ab264220)
All lanes: Western blot - Anti-PRKAR2 antibody (ab264220) at 0.04 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: HeLa whole cell lysate at 5 µg
Lane 4: HEK-293T whole cell lysate at 50 µg
Developed using the ECL technique.
Exposure time: 30s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2 antibody (ab264220)
Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for PRKAR2 using ab264220 at 1/200 dilution in immunohistochemical analysis. Detection: DAB.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com