AB75996

Anti-PRKAR2B antibody [EP2648]

RabMAb
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(5 Publications)

Rabbit Monoclonal PRKAR2B antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 5 publications.

View Alternative Names

cAMP-dependent protein kinase type II-beta regulatory subunit, PRKAR2B

3 Images

Flow Cytometry (Intracellular) - Anti-PRKAR2B antibody [EP2648] (AB75996)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-PRKAR2B antibody [EP2648] (AB75996)

Overlay histogram showing SHSY-5Y cells stained with ab75996 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75996 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor^® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2B antibody [EP2648] (AB75996)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2B antibody [EP2648] (AB75996)

ab75996, at 1/100 dilution, staining PRKAR2B in paraffin-embedded human brain tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-PRKAR2B antibody [EP2648] (AB75996)

All lanes:

Western blot - Anti-PRKAR2B antibody [EP2648] (ab75996) at 1/20000 dilution

Lane 1:

Fetal brain lysate at 10 µg

Lane 2:

Human testis lysate at 10 µg

Lane 3:

NIH/3T3 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 46 kDa

Observed band size: 46 kDa

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Carrier free

Anti-PRKAR2B antibody [EP2648] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2648

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

Flow Cyt (Intra), WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PRKAR2B also known as cAMP-dependent protein kinase type II-beta regulatory subunit plays a mechanical role by regulating the activity of the protein kinase A (PKA) holoenzyme. With a molecular mass of approximately 46 kDa PRKAR2B serves in anchoring the catalytic subunits in an inactive state in the cytosol. This protein is expressed in various tissues with particularly high levels in the brain. Its presence helps in modulating cellular response to fluctuating levels of cyclic adenosine monophosphate (cAMP).

Biological function summary

PRKAR2B holds a significant role in modulating signal transduction pathways by forming a complex with the catalytic subunits of PKA. This complex becomes active when cAMP binds to the regulatory subunit leading to the release and activation of the catalytic subunits. PRKAR2B's ability to anchor the catalytic subunits places it in central roles in processes like synaptic plasticity and memory formation. It is essential in processes that require precise control of PKA activity allowing response to external stimuli.

Pathways

PRKAR2B integrates into the cAMP signaling pathway and is impactful in the mitogen-activated protein kinase (MAPK) pathway. In the cAMP signaling pathway PRKAR2B regulates the activity of PKA which can phosphorylate numerous target proteins that affect transcription and metabolic regulation. PRKAR2B also interacts with proteins such as AKAP79/150 which target PKA to specific subcellular locations aligning closely with its biological functions within critical pathways.

Alterations in PRKAR2B function or expression have implications in neurological disorders such as schizophrenia and mood disorders. An imbalance in its activity can lead to disrupted cAMP signaling which affects neuronal communication and function. It is related to disorders through its interaction with other proteins such as DARPP-32 which also link to dopaminergic signaling pathways often implicated in these conditions. Research continues to explore PRKAR2B's role in these diseases providing greater understanding of its pathophysiological contributions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.

See full target information cAMP-dependent protein kinase type II-beta regulatory subunit

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of pain research 12:1287-1296 PubMed31114308

2019

Intrathecal TRPM8 blocking attenuates cold hyperalgesia via PKC and NF-κB signaling in the dorsal root ganglion of rats with neuropathic pain.

Applications

Unspecified application

Species

Unspecified reactive species

Song Cao,Qingmei Li,Jingfeng Hou,Zhourui Li,Xinya Cao,Xiaohong Liu,Bangyong Qin

PloS one 14:e0214606 PubMed30990811

2019

PID1 alters the antilipolytic action of insulin and increases lipolysis via inhibition of AKT/PKA pathway activation.

Applications

Unspecified application

Species

Unspecified reactive species

Chunyan Yin,Wei Hua Liu,Yuesheng Liu,Li Wang,Yanfeng Xiao

Journal of cellular physiology 234:13169-13181 PubMed30666648

2019

microRNA-665 silencing improves cardiac function in rats with heart failure through activation of the cAMP signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Lin,De-Guang Feng,Jing Xu

Molecular human reproduction 23:827-841 PubMed29069483

2017

Protein profile of mouse ovarian follicles grown in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Amandine Anastácio,Kenny A Rodriguez-Wallberg,Solenne Chardonnet,Cédric Pionneau,Christian Fédérici,Teresa Almeida Santos,Catherine Poirot

The Journal of neuroscience : the official journal of the Society for Neuroscience 32:7734-44 PubMed22649251

2012

Soluble adenylyl cyclase activity is necessary for retinal ganglion cell survival and axon growth.

Applications

IHC-Fr

Species

Rat

Raul G Corredor,Ephraim F Trakhtenberg,Wolfgang Pita-Thomas,Xiaolu Jin,Ying Hu,Jeffrey L Goldberg

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PRKAR2B antibody [EP2648]

Flow Cytometry (Intracellular) - Anti-PRKAR2B antibody [EP2648] (AB75996)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2B antibody [EP2648] (AB75996)

Western blot - Anti-PRKAR2B antibody [EP2648] (AB75996)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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