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AB284731

Anti-PRKAR2B antibody [EP2648] - BSA and Azide free

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Rabbit Monoclonal PRKAR2B antibody. Carrier free. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Mouse, Human samples.

View Alternative Names

cAMP-dependent protein kinase type II-beta regulatory subunit, PRKAR2B

3 Images
Flow Cytometry (Intracellular) - Anti-PRKAR2B antibody [EP2648] - BSA and Azide free (AB284731)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-PRKAR2B antibody [EP2648] - BSA and Azide free (AB284731)

This data was developed using ab75996, the same antibody clone in a different buffer formulation.Overlay histogram showing SHSY-5Y cells stained with ab75996 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75996, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2B antibody [EP2648] - BSA and Azide free (AB284731)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2B antibody [EP2648] - BSA and Azide free (AB284731)

This data was developed using ab75996, the same antibody clone in a different buffer formulation.ab75996, at 1/100 dilution, staining PRKAR2B in paraffin-embedded human brain tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-PRKAR2B antibody [EP2648] - BSA and Azide free (AB284731)
  • WB

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Western blot - Anti-PRKAR2B antibody [EP2648] - BSA and Azide free (AB284731)

This data was developed using ab75996, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PRKAR2B antibody [EP2648] (<a href='/en-us/products/primary-antibodies/prkar2b-antibody-ep2648-ab75996'>ab75996</a>) at 1/20000 dilution

Lane 1:

Fetal brain lysate at 10 µg

Lane 2:

Human testis lysate at 10 µg

Lane 3:

NIH/3T3 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 46 kDa

Observed band size: 46 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2648

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

IHC-P, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab284731 is the carrier-free version of ab75996

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PRKAR2B also known as cAMP-dependent protein kinase type II-beta regulatory subunit plays a mechanical role by regulating the activity of the protein kinase A (PKA) holoenzyme. With a molecular mass of approximately 46 kDa PRKAR2B serves in anchoring the catalytic subunits in an inactive state in the cytosol. This protein is expressed in various tissues with particularly high levels in the brain. Its presence helps in modulating cellular response to fluctuating levels of cyclic adenosine monophosphate (cAMP).
Biological function summary

PRKAR2B holds a significant role in modulating signal transduction pathways by forming a complex with the catalytic subunits of PKA. This complex becomes active when cAMP binds to the regulatory subunit leading to the release and activation of the catalytic subunits. PRKAR2B's ability to anchor the catalytic subunits places it in central roles in processes like synaptic plasticity and memory formation. It is essential in processes that require precise control of PKA activity allowing response to external stimuli.

Pathways

PRKAR2B integrates into the cAMP signaling pathway and is impactful in the mitogen-activated protein kinase (MAPK) pathway. In the cAMP signaling pathway PRKAR2B regulates the activity of PKA which can phosphorylate numerous target proteins that affect transcription and metabolic regulation. PRKAR2B also interacts with proteins such as AKAP79/150 which target PKA to specific subcellular locations aligning closely with its biological functions within critical pathways.

Alterations in PRKAR2B function or expression have implications in neurological disorders such as schizophrenia and mood disorders. An imbalance in its activity can lead to disrupted cAMP signaling which affects neuronal communication and function. It is related to disorders through its interaction with other proteins such as DARPP-32 which also link to dopaminergic signaling pathways often implicated in these conditions. Research continues to explore PRKAR2B's role in these diseases providing greater understanding of its pathophysiological contributions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
See full target information cAMP-dependent protein kinase type II-beta regulatory subunit
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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