AB75993

Anti-PRKAR2B antibody [EP2649Y]

BOND RX™ Validated
RabMAb
Recombinant
20ul selling size
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(24 Publications)

Rabbit Recombinant Monoclonal PRKAR2B antibody. Suitable for IHC-P, ICC/IF, IP, WB and reacts with Mouse, Rat, Human samples. Cited in 24 publications.

View Alternative Names

cAMP-dependent protein kinase type II-beta regulatory subunit, PRKAR2B

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2B antibody [EP2649Y] (AB75993)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2B antibody [EP2649Y] (AB75993)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fat tissue sections labeling PRKAR2B with purified ab75993 at 1/1500 dilution (0.27 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

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Immunoprecipitation - Anti-PRKAR2B antibody [EP2649Y] (AB75993)

Purified ab75993 at 1/30 dilution (2μg) immunoprecipitating PRKAR2B in Human brain lysate.
Lane 1 (input) : Human brain lysate 10μg
Lane 2 (+) : ab75993 + Human brain lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab75993 in Human brain lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 52 kDa

All lanes:

Immunoprecipitation - Anti-PRKAR2B antibody [EP2649Y] (ab75993)

Predicted band size: 46 kDa

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IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2B antibody [EP2649Y] (AB75993)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse fat tissue sections labeling PRKAR2B with purified ab75993 at 1/1500 dilution (0.27 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2B antibody [EP2649Y] (AB75993)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat fat tissue sections labeling PRKAR2B with purified ab75993 at 1/1500 dilution (0.27 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Unknown

Western blot - Anti-PRKAR2B antibody [EP2649Y] (AB75993)

All lanes:

Western blot - Anti-PRKAR2B antibody [EP2649Y] (ab75993) at 1/1000 dilution

Lane 1:

Human brain lysate at 15 µg

Lane 2:

Mouse brain lysate at 20 µg

Lane 3:

Rat brain lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 46 kDa

Observed band size: 52 kDa

false

Carrier free

Anti-PRKAR2B antibody [EP2649Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2649Y

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1500", "IHCP-species-notes": "For unpurified use at 1/100 - 1/250.Use of HRP conjugated or polymerized HRP secondary antibodies recommended. Stronger signals have been found using the polymerized HRP secondary. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "For unpurified use at 1/25000 - 1/50000." }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1500", "IHCP-species-notes": "For unpurified use at 1/100 - 1/250.Use of HRP conjugated or polymerized HRP secondary antibodies recommended. Stronger signals have been found using the polymerized HRP secondary. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1500", "IHCP-species-notes": "For unpurified use at 1/100 - 1/250.Use of HRP conjugated or polymerized HRP secondary antibodies recommended. Stronger signals have been found using the polymerized HRP secondary. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PRKAR2B also known as cAMP-dependent protein kinase type II-beta regulatory subunit plays a mechanical role by regulating the activity of the protein kinase A (PKA) holoenzyme. With a molecular mass of approximately 46 kDa PRKAR2B serves in anchoring the catalytic subunits in an inactive state in the cytosol. This protein is expressed in various tissues with particularly high levels in the brain. Its presence helps in modulating cellular response to fluctuating levels of cyclic adenosine monophosphate (cAMP).

Biological function summary

PRKAR2B holds a significant role in modulating signal transduction pathways by forming a complex with the catalytic subunits of PKA. This complex becomes active when cAMP binds to the regulatory subunit leading to the release and activation of the catalytic subunits. PRKAR2B's ability to anchor the catalytic subunits places it in central roles in processes like synaptic plasticity and memory formation. It is essential in processes that require precise control of PKA activity allowing response to external stimuli.

Pathways

PRKAR2B integrates into the cAMP signaling pathway and is impactful in the mitogen-activated protein kinase (MAPK) pathway. In the cAMP signaling pathway PRKAR2B regulates the activity of PKA which can phosphorylate numerous target proteins that affect transcription and metabolic regulation. PRKAR2B also interacts with proteins such as AKAP79/150 which target PKA to specific subcellular locations aligning closely with its biological functions within critical pathways.

Alterations in PRKAR2B function or expression have implications in neurological disorders such as schizophrenia and mood disorders. An imbalance in its activity can lead to disrupted cAMP signaling which affects neuronal communication and function. It is related to disorders through its interaction with other proteins such as DARPP-32 which also link to dopaminergic signaling pathways often implicated in these conditions. Research continues to explore PRKAR2B's role in these diseases providing greater understanding of its pathophysiological contributions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.

See full target information cAMP-dependent protein kinase type II-beta regulatory subunit

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Publications (24)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:651 PubMed39809799

2025

Myristoylated Eepd1 Enhances Lipolysis and Thermogenesis through PKA Activation to Combat Obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Suzhen Chen,Yanping Wang,Qian Zhou,Qiqi Qian,Quanxin Jiang,Chuchu Liu,Yan Liu,Peihui Zhou,Jie Xiong,Yao Zhang,Ning Wang,Yang Emma Li,Limin Yin,Hongyuan Yang,Junli Liu

Neuroscience bulletin 39:194-212 PubMed35802246

2022

miR-34b-3p Inhibition of eIF4E Causes Post-stroke Depression in Adult Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Ke,Manfei Deng,Zhuoze Wu,Hongyan Yu,Dian Yu,Hao Li,Youming Lu,Kai Shu,Lei Pei

Environmental toxicology 37:2072-2083 PubMed35524977

2022

Long noncoding RNA TDRG1 aggravates doxorubicin-induced cardiomyopathy by binding with miR-873-5p to upregulate PRKAR2.

Applications

Unspecified application

Species

Unspecified reactive species

Yihang Liu,Linlin Tan,Ming Zhang,Chuang Yang

Frontiers in molecular neuroscience 14:782041 PubMed34867193

2021

Protein Kinase A in Human Retina: Differential Localization of Cβ, Cα, RIIα, and RIIβ in Photoreceptors Highlights Non-redundancy of Protein Kinase A Subunits.

Applications

Unspecified application

Species

Unspecified reactive species

Jinae N Roa,Yuliang Ma,Zbigniew Mikulski,Qianlan Xu,Ronit Ilouz,Susan S Taylor,Dorota Skowronska-Krawczyk

The Journal of biological chemistry : PubMed32855239

2020

Induction of UCP1 and thermogenesis by a small molecule via AKAP1/PKA modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Laurent Vergnes,Jason Y Lin,Graeme R Davies,Christopher D Church,Karen Reue

Food science & nutrition 8:3515-3524 PubMed32724614

2020

Nobiletin inhibits cell growth through restraining aerobic glycolysis via PKA-CREB pathway in oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Chong-Xiang Lin,Cheng-Wei Tu,Yi-Ke Ma,Peng-Cheng Ye,Xia Shao,Zhao-An Yang,Yi-Ming Fang

Journal of cellular and molecular medicine 24:7000-7014 PubMed32394486

2020

A R inhibition in alleviating spatial recognition memory impairment after TBI is associated with improvement in autophagic flux in RSC.

Applications

Unspecified application

Species

Unspecified reactive species

Xu-Jia Zeng,Ping Li,Ya-Lei Ning,Yan Zhao,Yan Peng,Nan Yang,Ya-Wei Xu,Jiang-Fan Chen,Yuan-Guo Zhou

Frontiers in neuroscience 13:1225 PubMed31798406

2019

High-Frequency Repetitive Transcranial Magnetic Stimulation Mediates Autophagy Flux in Human Bone Mesenchymal Stromal Cells via NMDA Receptor-Ca-Extracellular Signal-Regulated Kinase-Mammalian Target of Rapamycin Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xinlong Wang,Xing Zhou,Jie Bao,Zhiguo Chen,Jingzhao Tang,Xueyang Gong,Jing Ni,Qi Fang,Yaobo Liu,Min Su

Journal of molecular cell biology 11:781-790 PubMed31220300

2019

Rheb promotes brown fat thermogenesis by Notch-dependent activation of the PKA signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Meng,Xiuci Liang,Ting Xiao,Jing Wang,Jie Wen,Hairong Luo,Jianhui Teng,Yanquan Fei,Qinghai Zhang,Bilian Liu,Fang Hu,Juli Bai,Meilian Liu,Zhiguang Zhou,Feng Liu

Artificial cells, nanomedicine, and biotechnology 47:2343-2351 PubMed31184227

2019

Ras-ERK signalling represses H1.4 phosphorylation at serine 36 to promote non-small-cell lung carcinoma cells growth and migration.

Applications

Unspecified application

Species

Unspecified reactive species

Shaomin Shi,Jingzhe Zhang,Meihan Liu,Hang Dong,Ning Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PRKAR2B antibody [EP2649Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2B antibody [EP2649Y] (AB75993)

Immunoprecipitation - Anti-PRKAR2B antibody [EP2649Y] (AB75993)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2B antibody [EP2649Y] (AB75993)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2B antibody [EP2649Y] (AB75993)

Western blot - Anti-PRKAR2B antibody [EP2649Y] (AB75993)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (24)

Product promise