Anti-PRKAR2B antibody [EP2649Y] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2B antibody [EP2649Y] - BSA and Azide free (AB239870)

This data was developed using ab75993, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fat tissue sections labeling PRKAR2B with purified ab75993 at 1/1500 dilution (0.27 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• IP

Unknown

Immunoprecipitation - Anti-PRKAR2B antibody [EP2649Y] - BSA and Azide free (AB239870)

This data was developed using ab75993, the same antibody clone in a different buffer formulation.
Purified ab75993 at 1/30 dilution (2μg) immunoprecipitating PRKAR2B in Human brain lysate.
Lane 1 (input) : Human brain lysate 10μg
Lane 2 (+) : ab75993 + Human brain lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab75993 in Human brain lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 52 kDa

All lanes:

Immunoprecipitation - Anti-PRKAR2B antibody [EP2649Y] (<a href='/en-us/products/primary-antibodies/prkar2b-antibody-ep2649y-ab75993'>ab75993</a>)

Predicted band size: 46 kDa

false

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2B antibody [EP2649Y] - BSA and Azide free (AB239870)

This data was developed using ab75993, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse fat tissue sections labeling PRKAR2B with purified ab75993 at 1/1500 dilution (0.27 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2B antibody [EP2649Y] - BSA and Azide free (AB239870)

This data was developed using ab75993, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat fat tissue sections labeling PRKAR2B with purified ab75993 at 1/1500 dilution (0.27 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• WB

Unknown

Western blot - Anti-PRKAR2B antibody [EP2649Y] - BSA and Azide free (AB239870)

This data was developed using ab75993, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PRKAR2B antibody [EP2649Y] (<a href='/en-us/products/primary-antibodies/prkar2b-antibody-ep2649y-ab75993'>ab75993</a>) at 1/1000 dilution

Lane 1:

Human brain lysate at 15 µg

Lane 2:

Mouse brain lysate at 20 µg

Lane 3:

Rat brain lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 46 kDa

Observed band size: 52 kDa

false